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This Article Full Text Full Text (PDF) All Versions of this Article: 290/6/H2267    most recent 01093.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Huang, J. Articles by Tibbits, G. F. Search for Related Content PubMed PubMed Citation Articles by Huang, J. Articles by Tibbits, G. F.

L-type Ca²⁺ channel function and expression in neonatal rabbit ventricular myocytes

¹Cardiac Membrane Research Laboratory, Simon Fraser University, Burnaby, British Columbia; ²Cardiovascular Sciences, Child and Family Research Institute, Vancouver, British Columbia, Canada; and ³Cell Physiology Laboratory, Cardiology, Hospital de Santa Creus y Sant Pau, Barcelona, Spain

Submitted 17 October 2005 ; accepted in final form 30 November 2005

L-type Ca²⁺ channel-mediated, Ca²⁺-induced Ca²⁺ release (CICR) is the dominant mode of excitation-contraction (E-C) coupling in the mature mammalian myocardium but is thought to be absent in the fetal and newborn mammalian myocardium. Furthermore, the characteristics and contributors of E-C coupling at the earliest developmental stages are poorly understood. In this study, we measured [³H](+)PN200-110 dihydropyridine binding capacity, functionality and expression of the L-type Ca²⁺ channel, and cytosolic [Ca²⁺] ([Ca²⁺]_i) at various developmental stages (3, 6, 10, 20, and 56 days old) to characterize ontogenetic changes in E-C coupling. We found that 1) the whole cell L-type Ca²⁺ channel peak current (I_Ca) density increased slightly in parallel with cell growth, but the current-voltage relationship, the steady-state activation, and the maximum DHP binding and binding affinity did not exhibit significant developmental changes; 2) sarcoplasmic reticulum Ca²⁺ dependence of inactivation rates of L-type Ca²⁺ channel and peak of I_Ca density were only observed after 10 days of age, which temporally coincides with transverse (T)-tubule formation; 3) the relationship between [Ca²⁺]_i and voltage changed from a linear relationship at the earliest developmental stages to a "bell-shaped" relationship at the later developmental stages, presumably corresponding to a switch from reverse-mode Na/Ca exchange-dependent to I_Ca-dependent E-C coupling; and 4) the expression of two different splice variants of Ca_V1.2, IVS3A and IVS3B, switched from predominantly IVS3A at the earliest stages to IVS3B at the later developmental stages. Our data suggest that whereas the density of functional dihydropyridine receptors (DHPRs) increases only slightly during ontogeny, the enhancement of functional coupling between DHPR and ryanodine receptor is dramatic between the second and third weeks after birth. Furthermore, we found that the differential expression of splice variants during development temporally correlated with the appearance of I_Ca-dependent E-C coupling and T-tubule formation.

neonate myocardium; contractility; excitation-contraction coupling; dihydropyridine receptor

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