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Am J Physiol Heart Circ Physiol 291: H193-H201, 2006. First published February 10, 2006; doi:10.1152/ajpheart.00802.2005
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Alterations in structure and mechanics of resistance arteries from ouabain-induced hypertensive rats

Ana M. Briones,1 Fabiano E. Xavier,1 Silvia M. Arribas,2 M. Carmen González,2 Luciana V. Rossoni,3 María J. Alonso,1,4 and Mercedes Salaices1

1Departamento de Farmacología y Terapéutica and 2Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain; 3Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, SP, Brazil; and 4Departamento de Ciencias de la Salud, Facultad de Ciencias de la Salud, Universidad Rey Juan Carlos, Alcorcón, Spain

Submitted 28 July 2005 ; accepted in final form 6 February 2006

We have previously described that chronic administration of ouabain induces hypertension and functional alterations in mesenteric resistance arteries. The aim of this study was to analyze whether ouabain treatment also alters the structural and mechanical properties of mesenteric resistance arteries. Wistar rats were treated for 5 wk with ouabain (8.0 µg/day sc). The vascular structure and mechanics of the third-order branches of the mesenteric artery were assessed with pressure myography and confocal microscopy. Total collagen content was determined by picrosirius red staining, collagen I/III was analyzed by Western blot, and elastin was studied by confocal microscopy. Vascular reactivity was analyzed by wire myography. Internal and external diameters and cross-sectional area were diminished, whereas the wall-to-lumen ratio was increased in arteries from ouabain-treated rats compared with controls. In addition, arteries from ouabain-treated rats were stiffer. Ouabain treatment decreased smooth muscle cell number and increased total and I/III collagens in the vascular wall. However, this treatment did not modify adventitia and media thickness, nuclei morphology, elastin structure, and vascular reactivity to norepinephrine and acetylcholine. The present work shows hypotrophic inward remodeling of mesenteric resistance arteries from ouabain-treated rats that seems to be the consequence of a combination of decreased cell number and impaired distension of the artery, possibly due to a higher stiffness associated with collagen deposition. The narrowing of resistance arteries could play a role in the pathogenesis of hypertension in this model.

extracellular matrix; hypertension; arterial remodeling


Address for reprint requests and other correspondence: M. Salaices, Dpto. Farmacología y Terapéutica, Facultad de Medicina, Univ. Autónoma de Madrid, C/Arzobispo Morcillo, E-28029 Madrid, Spain (e-mail: mercedes.salaices{at}uam.es)




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