HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 291/3/H1236    most recent 00838.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Sorop, O. Articles by VanBavel, E. Search for Related Content PubMed PubMed Citation Articles by Sorop, O. Articles by VanBavel, E.

Calcium channel blockade prevents pressure-dependent inward remodeling in isolated subendocardial resistance vessels

Department of Medical Physics, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

Submitted 5 August 2005 ; accepted in final form 3 March 2006

The capacity for myocardial perfusion depends on the structure of the coronary microvascular bed. Coronary microvessels may adapt their structure to various stimuli. We tested whether the local pressure profile affects tone and remodeling of porcine coronary microvessels. Subendocardial vessels (160 µm, n = 53) were cannulated and kept in organoid culture for 3 days under different transvascular pressure profiles: Osc 80: mean 80 mmHg, 60 mmHg peak-peak sine wave pulsation amplitude at 1.5 Hz; St 80: steady 80 mmHg; Osc 40: mean 40 mmHg, 30 mmHg amplitude; St 40: steady 40 mmHg. Under the Osc 80 profile, modest tone developed, reducing the diameter to 81 ± 14% (mean ± SE, n = 6) of the maximal, passive diameter. No inward remodeling was found here, as determined from the passive pressure-diameter relation after 3 days of culture. Under all other profiles, much more tone developed (e.g., Osc 40: to 26 ± 3%, n = 7). In addition, these vessels showed eutrophic (i.e., without a change in wall cross-sectional area) inward remodeling (e.g., Osc 40: passive diameter reduction by 24 ± 3%). The calcium blocker amlodipine induced maintained dilation in St 40 vessels and reversed the 22 ± 3% (n = 6) inward remodeling to 15 ± 3% (n = 8) outward remodeling toward day 3. Vessels required a functional endothelium to maintain structural integrity in culture. Our data indicate that reduction of either mean pressure or pulse pressure leads to microvascular constriction followed by inward remodeling. These effects could be reversed by amlodipine. Although microvascular pressure profiles distal to stenoses are poorly defined, these data suggest that vasodilator therapy could improve subendocardial microvascular function and structure in coronary artery disease.

coronary circulation; microcirculation; vascular remodeling; amlodipine

This article has been cited by other articles:

				O. Sorop, D. Merkus, V. J. de Beer, B. Houweling, A. Pistea, E. O. McFalls, F. Boomsma, H. M. van Beusekom, W. J. van der Giessen, E. VanBavel, et al. Functional and Structural Adaptations of Coronary Microvessels Distal to a Chronic Coronary Artery Stenosis Circ. Res., April 11, 2008; 102(7): 795 - 803. [Abstract] [Full Text] [PDF]

				J. C. B. Jacobsen, M. J. Mulvany, and N.-H. Holstein-Rathlou A mechanism for arteriolar remodeling based on maintenance of smooth muscle cell activation Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2008; 294(4): R1379 - R1389. [Abstract] [Full Text] [PDF]

				C. A. A. Hulsman, J. R. Vingerling, A. Hofman, J. C. M. Witteman, and P. T. V.M. de Jong Blood Pressure, Arterial Stiffness, and Open-angle Glaucoma: The Rotterdam Study Arch Ophthalmol, June 1, 2007; 125(6): 805 - 812. [Abstract] [Full Text] [PDF]