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This Article Full Text Full Text (PDF) All Versions of this Article: 291/3/H1255    most recent 01303.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Gao, Z. Articles by Li, J. Search for Related Content PubMed PubMed Citation Articles by Gao, Z. Articles by Li, J.

Temperature modulates P2X receptor-mediated cardiovascular responses to muscle afferent activation

Heart and Vascular Institute and Division of Cardiology, Department of Medicine, Penn State College of Medicine, Milton S. Hershey Medical Center, Hershey, Pennsylvania

Submitted 12 December 2005 ; accepted in final form 24 February 2006

Static muscle contraction increases ATP release into the muscle interstitial space. Elevated ATP in muscle stimulates thin fiber muscle afferents and increases blood pressure via engagement of purinergic P2X receptors. In addition, ATP activates P2X receptors and enhances cardiovascular responses induced by stimulation of muscle mechanoreceptors. In this study, we examined whether elevated muscle temperature would attenuate and whether reduced temperature would potentiate P2X effects on reflex muscle responses. ,-Methylene ATP (,-MeATP) was injected into the arterial blood supply of hindlimb muscle to stimulate P2X receptors, and muscle stretch was induced to activate mechanically sensitive muscle afferents as ,-MeATP was injected in 10 anesthetized cats. Femoral arterial injection of ,-MeATP (1.0 mM) increased mean arterial pressure (MAP) by 35 ± 5 (35°C), 26 ± 3 (37°C), and 19 ± 3 mmHg (39°C; P < 0.05 vs. 35°C), respectively. Muscle stretch (2 kg) elevated MAP. The MAP response was significantly enhanced 34% and 36% when ,-MeATP (0.2 mM) was arterially infused 5 min before muscle stretch at 35° and 37°C, respectively. However, as muscle temperature reached 39°C, the stretch-evoked response was augmented only 6% by ,-MeATP injection, and the response was significantly attenuated compared with the response with muscle temperature of 35° and 37°C. In addition, we also examined effects of muscle temperature on ,-MeATP enhancement of the cardiovascular responses to static muscle contraction while the muscles were freely perfused and the circulation to the muscles was occluded. Because muscle temperature was 37°C, arterial injections of ,-MeATP significantly augmented contraction-evoked MAP response by 49% (freely perfused) and 53% (ischemic condition), respectively. It is noted that this effect was significantly attenuated at a muscle temperature of 39°C. These data indicate that the effect of P2X receptor on reflex muscle response is sensitive to alternations of muscle temperature and that elevated temperature attenuates the response.

adenosine 5'-triphosphate; muscle stretch; muscle temperature; muscle pressor reflex; blood pressure

This article has been cited by other articles:

				Z. Gao, J. D. Li, L. I. Sinoway, and J. Li Effect of muscle interstitial pH on P2X and TRPV1 receptor-mediated pressor response J Appl Physiol, June 1, 2007; 102(6): 2288 - 2293. [Abstract] [Full Text] [PDF]