HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 291/6/H2905    most recent 00323.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Belke, D. D. Articles by Dillmann, W. H. Search for Related Content PubMed PubMed Citation Articles by Belke, D. D. Articles by Dillmann, W. H.

In vivo gene delivery of HSP70i by adenovirus and adeno-associated virus preserves contractile function in mouse heart following ischemia-reperfusion

¹Department of Medicine, University of California, San Diego, La Jolla, California; and ²Division of Exercise Physiology, West Virginia University, Morgantown, West Virginia

Submitted 28 March 2006 ; accepted in final form 1 August 2006

Inducible heat shock protein 70 (HSP70i) has been shown to exert a protective effect in hearts subjected to ischemia-reperfusion. Although studied in heat-shocked animals and in transgenic mice that constitutively overexpress the protein, the therapeutic application of the protein in the form of a viral vector-mediated HSP70i expression has not been widely examined. Accordingly, we have examined the effects of HSP70i delivered in vivo to the left ventricular free wall of the heart via viral gene therapy in mice. The affect of virally mediated HSP70i expression in preserving cardiac function following ischemia-reperfusion was examined after short-term expression (5-day adenovirus mediated) and long-term expression (8-mo adeno-associated virus mediated) in mice by subjecting ex vivo Langendorff perfused hearts to a regime of ischemia-reperfusion. Both vectors were capable of increasing HSP70i expression in the heart, and neither vector had any effect on cardiac function during aerobic (preischemic) perfusion when compared with corresponding controls. In contrast, both adenovirus-mediated and adeno-associated virus-mediated expression of HSP70i improved the contractile recovery of the heart after 120 min of reperfusion following ischemia. This study demonstrates the feasibility of using both short- and long-term expression of virally mediated HSP70i as a therapeutic intervention against cardiac ischemia-reperfusion injury.

inducible heat shock protein 70; left ventricle

This article has been cited by other articles:

				T. Matsuda, P. Zhai, Y. Maejima, C. Hong, S. Gao, B. Tian, K. Goto, H. Takagi, M. Tamamori-Adachi, S. Kitajima, et al. Distinct roles of GSK-3{alpha} and GSK-3{beta} phosphorylation in the heart under pressure overload PNAS, December 30, 2008; 105(52): 20900 - 20905. [Abstract] [Full Text] [PDF]

				T. M. Vondriska and Y. Wang A New (Heat) Shocking Player in Cardiac Hypertrophy Circ. Res., November 21, 2008; 103(11): 1194 - 1196. [Full Text] [PDF]

				P. S. Pagel Induction of Heat Shock Protein 70 and Preconditioning by Sevoflurane: A Potent Protective Interaction Against Myocardial Ischemia-Reperfusion Injury Anesth. Analg., September 1, 2008; 107(3): 742 - 745. [Full Text] [PDF]