HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 292/1/H369    most recent 00704.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Joho, S. Articles by Grossman, W. Search for Related Content PubMed PubMed Citation Articles by Joho, S. Articles by Grossman, W.

Left ventricular pressure-volume relationship in conscious mice

¹Cardiology Division, Department of Medicine, University of California; and ²Veterans Affairs Medical Center, San Francisco, California

Submitted 3 July 2006 ; accepted in final form 7 August 2006

With the availability of transgenic models, the mouse has become an increasingly important subject for genetic-hemodynamic studies. Recently, we developed a technique to measure left ventricular (LV) pressure in conscious mice with an implanted LV polyethylene tube. We extended our new method by evaluating the LV pressure-volume relationship and examined the feasibility of this method in this study. We studied 17 male mice (age, 11–20 wk) with a conductance catheter inserted into the LV through the polyethylene tube. Load-independent parameters of contractility derived from pressure-volume relationship [slope of the end-systolic pressure-volume relationship (E_es), slope of the maximum first derivative of LV pressure (dP/dt_max)-end-diastolic volume (EDV) relation, and preload-recruitable stroke work (PRSW)] were evaluated by inferior vena caval occlusion with an implanted snare. LV function assessed by this technique on two different days showed that the parameters were very similar, indicating reproducibility. Both linear and nonlinear regression analyses were performed for E_es. Contractility was enhanced by isoproterenol (E_es, 13.1 ± 6.6 to 20.8 ± 8.7 mmHg/µl; dP/dt_max-EDV, 496 ± 139 to 825 ± 178 mmHg·s^–1·µl^–1; and PRSW, 110 ± 23 to 127 ± 21 mmHg), depressed by atenolol (E_es, 14.5 ± 6.1 to 4.6 ± 2.0 mmHg/µl; dP/dt_max-EDV, 543 ± 188 to 185 ± 94 mmHg·s^–1·µl^–1; and PRSW, 117 ± 20 to 70 ± 15 mmHg) and isoflurane (E_es, 12.3 ± 6.0 to 5.7 ± 2.1 mmHg/µl; dP/dt_max-EDV, 528 ± 172 to 164 ± 68 mmHg/s·µl; and PRSW, 124 ± 19 to 48 ± 10 mmHg), significantly. In conclusion, this is the first description of the LV pressure-volume relationship in conscious mice. These findings suggest that this method is feasible to detect changes of contractility in the conscious state, allowing serial assessment of pressure-volume-derived cardiac function indexes over time without anesthesia or repeated surgery.

ventricular function; mouse physiology

This article has been cited by other articles:

				P. Pokreisz, G. Marsboom, and S. Janssens Pressure overload-induced right ventricular dysfunction and remodelling in experimental pulmonary hypertension: the right heart revisited Eur. Heart J. Suppl., December 1, 2007; 9(suppl_H): H75 - H84. [Abstract] [Full Text] [PDF]

				D. J. Chess, B. Lei, B. D. Hoit, A. M. Azimzadeh, and W. C. Stanley Deleterious effects of sugar and protective effects of starch on cardiac remodeling, contractile dysfunction, and mortality in response to pressure overload Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1853 - H1860. [Abstract] [Full Text] [PDF]