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This Article Full Text Full Text (PDF) All Versions of this Article: 292/3/H1254    most recent 01058.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Wisel, S. Articles by Kuppusamy, P. Search for Related Content PubMed PubMed Citation Articles by Wisel, S. Articles by Kuppusamy, P.

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Oxygen Sensing: Life and Death of a Cell

Labeling of skeletal myoblasts with a novel oxygen-sensing spin probe for noninvasive monitoring of in situ oxygenation and cell therapy in heart

Center for Biomedical Electron Paramagnetic Resonance Spectroscopy and Imaging, ¹Division of Cardiothoracic Surgery, Department of Surgery; ²Division of Cardiovascular Medicine, Department of Internal Medicine; and ³Department of Neuroscience, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio

Submitted 26 September 2006 ; accepted in final form 27 November 2006

We report the labeling (internalization) of skeletal myoblasts (SMs) with a novel class of oxygen-sensing paramagnetic spin probe for noninvasive tracking and in situ monitoring of oxygenation in stem cell therapy using electron paramagnetic resonance (EPR) spectroscopy. SM cells were isolated from thigh muscle biopsies of mice and propagated in culture. Labeling of SM cells with the probe was achieved by coincubating the cells with submicron-sized (270 ± 120 nm) particulates of the probe in culture for 48 h. The labeling had no significant effect on the viability or proliferation of the cells. The SM cells labeled with the probe were transplanted in the infarcted region of mouse hearts. The engraftment of the transplanted cells in the infarct region was verified by using MY-32 staining for skeletal myocytes. The in situ PO₂ in the heart was determined noninvasively and repeatedly for 4 wk after transplantation. The results showed significant enhancement of myocardial oxygenation at the site of cell transplant compared with untreated control. In conclusion, labeling of SM cells with the oxygen-sensing spin probe offers a unique opportunity for the noninvasive monitoring of transplanted cells as well as in situ tissue PO₂ in infarcted mouse hearts.

electron paramagnetic resonance spectroscopy; myocardial infarction; lithium octa-n-butoxy-naphthalocyanine; OxySpin

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