HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 292/4/H1821    most recent 00365.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Karlsen, T. V. Articles by Reed, R. K. Search for Related Content PubMed PubMed Citation Articles by Karlsen, T. V. Articles by Reed, R. K.

Lowering of interstitial fluid pressure after neurogenic inflammation in mouse skin is partly dependent on mast cells

Department of Biomedicine, University of Bergen, Bergen, Norway

Submitted 6 April 2006 ; accepted in final form 27 November 2006

Neurogenic inflammation is known to induce lowering of interstitial fluid pressure (P_if) in mouse skin. This study examined the possible role of mast cell activation secondary to neuropeptide release in lowering of P_if by using Kit^W/Kit^W-v mice, which are devoid of mast cells, including connective tissue mast cells (CTMCs). P_if was measured in paw skin of anesthetized (fentanyl-fluanison and midazolam, 1:1) mice with glass capillaries connected to a servo-controlled counterpressure system. In contrast to wild-type mice, intravenous administration of mast cell-activating compound 48/80 induced no lowering of P_if in Kit^W/Kit^W-v mice. Intravenous challenge with substance P (SP), calcitonin gene-related peptide (CGRP), or capsaicin induced a significant (P < 0.05) lowering of P_if in wild-type mice to –2.16 ± 0.28, –1.96 ± 0.11, and –2.22 ± 0.19 mmHg, respectively, compared with vehicle (–0.49 ± 0.11 mmHg). In Kit^W/Kit^W-v mice the P_if response to SP was completely abolished (–0.53 ± 0.32 mmHg) while the response to CGRP and capsaicin was attenuated (–1.33 ± 0.13 and –1.42 ± 0.13 mmHg, respectively) although significantly (P < 0.05) lowered compared with vehicle. Immunohistochemical analysis revealed no difference in distribution or density of SP- and CGRP-immunoreactive fibers in paws of Kit^W/Kit^W-v compared with wild-type mice. We conclude that lowering of P_if normally depends on mast cells. However, the sensory nerves can also elicit a lowering of P_if that is independent of mast cells.

Kit^W/Kit^W-v mice; neuropeptides; micropuncture

This article has been cited by other articles:

				R. M. Dongaonkar, C. M. Quick, R. H. Stewart, R. E. Drake, C. S. Cox Jr., and G. A. Laine Edemagenic gain and interstitial fluid volume regulation Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R651 - R659. [Abstract] [Full Text] [PDF]