AJP - Heart Calcium Transients and Cell-Sarcomere
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Am J Physiol Heart Circ Physiol 293: H193-H203, 2007. First published March 2, 2007; doi:10.1152/ajpheart.00728.2006
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Hic-5 promotes endothelial cell migration to lysophosphatidic acid

C. Avraamides,1,2 M. E. Bromberg,2,3 J. P. Gaughan,4 S. M. Thomas,6 A. Y. Tsygankov,1,5 and T. S. Panetti1,2

1Department of Microbiology and Immunology, 2Sol Sherry Thrombosis Research Center, 3Department of Medicine, 4Biostatistics Consulting Center, 5Fels Institute For Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania; and 6Cancer Biology Program, Harvard Medical School, Boston, Massachusetts

Submitted 7 July 2006 ; accepted in final form 22 February 2007

Endothelial cell migration is critical for proper blood vessel development. Signals from growth factors and matrix proteins are integrated through focal adhesion proteins to alter cell migration. Hydrogen peroxide-inducible clone 5 (Hic-5), a paxillin family member, is enriched in the focal adhesions in bovine pulmonary artery endothelial (BPAE) cells, which migrate to lysophosphatidic acid (LPA) on denatured collagen. In this study, we investigate the role of Hic-5 in LPA-stimulated endothelial cell migration. LPA recruits Hic-5 to the focal adhesions and to the pseudopodia in BPAE cells plated on collagen, suggesting that recruitment of Hic-5 to focal adhesions is associated with endothelial cell migration. Knockdown of endogenous Hic-5 significantly decreases migration toward LPA, confirming involvement of Hic-5 in migration. To address the role of Hic-5 in endothelial cell migration, we exogenously expressed wild-type (WT) Hic-5 and green fluorescent protein Hic-5 C369A/C372A (LIM3 mutant) constructs in BPAE cells. WT Hic-5 expression increases chemotaxis of BPAE cells to LPA, whereas migration toward LPA of the green fluorescent protein Hic-5 C369A/C372A-expressing cells is similar to that shown in vector control cells. Additionally, ERK phosphorylation is enhanced in the presence of LPA in WT Hic-5 cells. A pharmacological inhibitor of MEK activity inhibits LPA-stimulated WT Hic-5 cell migration and ERK phosphorylation, suggesting Hic-5 enhances migration via MEK activation of ERK. Together, these studies indicate that Hic-5, a focal adhesion protein in endothelial cells, is recruited to the pseudopodia in the presence of LPA and enhances migration.

pseudopodia; focal adhesion proteins; angiogenesis


Address for reprint requests and other correspondence: T. S. Panetti, Dept. of Microbiology and Immunology and Thrombosis Research Center, Temple Univ. School of Medicine, 3400 N. Broad St., Philadelphia, PA 19140 (e-mail: tspanetti{at}yahoo.com)






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