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Acute myocardial infarction in mice: assessment of transmurality by strain rate imaging

¹Institut National de la Santé et de la Recherche Médicale E 0226, Université Claude Bernard Lyon I and ²Hôpital Louis Pradel, Hospices Civils de Lyon, Lyon, ³Hôpital Pontchaillou, Rennes, and ⁴Hôpital Charles Nicolle, Rouen, France; and ⁵Massachusetts General Hospital, Boston, Massachusetts

Submitted 20 January 2007 ; accepted in final form 19 March 2007

In vivo evaluation of the transmural extension of myocardial infarction (TEI) is crucial to prediction of viability and prognosis. With the rise of transgenic technology, murine myocardial infarction (MI) models are increasingly used. Our study aimed to evaluate systolic strain rate (SR), a new parameter of regional function, to quantify TEI in a murine model of acute MI induced by various durations of ischemia followed by 24 h of reperfusion. Global and regional left ventricular (LV) function were assessed by echocardiography (13 MHz, Vivid 7, GE) in 4 groups of wild-type mice (C57BL/6, 2 mo old): a sham-treated group (n = 10) and three MI groups [30 (n = 11), 60 (n = 10), and 90 (n = 9) min of left coronary artery occlusion]. Conventional LV dimensions, anterior wall (AW) thickening, and peak systolic SR were measured before and 24 h after reperfusion. Area at risk (AR) was measured by blue dye and infarct size (area of necrosis, AN) and TEI by triphenyltetrazolium chloride staining. AN increased with ischemia duration (25 ± 2%, 56 ± 5%, 71 ± 6% of AR for 30, 60, and 90 min, respectively; P < 0.05). LV end-diastolic volume significantly increased with ischemia duration (30 ± 5, 34 ± 5, 43 ± 5 µl; P < 0.05), whereas LV ejection fraction decreased (63 ± 5%, 58 ± 6%, 46 ± 5%; P < 0.05). AW thickening decrease was not influenced by ischemia duration. Conversely, systolic SR decreased with ischemia duration (13 ± 5, 4 ± 3, –2 ± 6 s^–1; P < 0.05) and was significantly correlated with TEI (r = 0.89, P < 0.01). Receiver operating characteristic (ROC) curves identified systolic SR as the most accurate parameter to predict TEI. In conclusion, in a murine model of MI, SR imaging is superior to conventional echocardiography to predict TEI early after MI.

echocardiography; murine model