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Am J Physiol Heart Circ Physiol 293: H866-H874, 2007. First published February 16, 2007; doi:10.1152/ajpheart.01055.2006
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INNOVATIVE METHODOLOGY

A modular instrument for exploring the mechanics of cardiac myocytes

M. G. Garcia-Webb,1 A. J. Taberner,2 N. C. Hogan,2 and I. W. Hunter2

Departments of 1Bioengineering and 2Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts

Submitted 26 September 2006 ; accepted in final form 16 February 2007

The cardiac ventricular myocyte is a key experimental system for exploring the mechanical properties of the diseased and healthy heart. Millions of primary myocytes, which remain viable for 4–6 h, can be readily isolated from animal models. However, currently available instrumentation allows the mechanical properties of only a few physically loaded myocytes to be explored within 4–6 h. Here we describe a modular and inexpensive prototype instrument that could form the basis of an array of devices for probing the mechanical properties of single mammalian myocytes in parallel. This device would greatly increase the throughput of scientific experimentation and could be applied as a high-content screening instrument in the pharmaceutical industry. The instrument module consists of two independently controlled Lorentz force actuators-force transducers in the form of 0.025 x 1 x 5 mm stainless steel cantilevers with 0.5 m/N compliance and 360-Hz resonant frequency. Optical position sensors focused on each cantilever provide position and force resolution of <1 nm/{surd}Hz and <2 nN/{surd}Hz, respectively. The motor structure can produce peak displacements and forces of ±200 µm and ±400 µN, respectively. Custom Visual Basic.Net software provides data acquisition, signal processing, and digital control of cantilever position. The functionality of the instrument was demonstrated by implementation of novel methodologies for loading and attaching healthy mammalian ventricular myocytes to the force sensor and actuator and use of stochastic system identification techniques to measure their passive dynamic stiffness at various sarcomere lengths.

muscle mechanics; dynamic stiffness; force sensor; actuator


Address for reprint requests and other correspondence: M. G. Garcia-Webb, 3-147 77 Massachusetts Ave., Cambridge, MA 02139






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