HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 293/3/H1883    most recent 00514.2007v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (16) Citing Articles via Google Scholar Google Scholar Articles by Banerjee, I. Articles by Baudino, T. A. Search for Related Content PubMed PubMed Citation Articles by Banerjee, I. Articles by Baudino, T. A.

Determination of cell types and numbers during cardiac development in the neonatal and adult rat and mouse

Cell and Developmental Biology and Anatomy, University of South Carolina School of Medicine, Columbia, South Carolina

Submitted 30 April 2007 ; accepted in final form 28 June 2007

Cardiac fibroblasts, myocytes, endothelial cells, and vascular smooth muscle cells are the major cellular constituents of the heart. The aim of this study was to observe alterations in myocardial cell populations during early neonatal development in the adult animal and to observe any variations of the cardiac cell populations in different species, specifically, the rat and mouse. Whole hearts were isolated from either mice or rats during the neonatal and adult stages of development, and single cell suspensions were prepared via sequential collagenase digestion. Heterogeneous cell populations were immunolabeled for specific cell types and analyzed using fluorescence-activated cell sorting (FACS). In addition, the left ventricle, right ventricle, and septa were isolated, fixed, and sectioned for morphometric analyses. These same cardiac regions were also analyzed using FACS. We observed that the adult murine myocardium is composed of 56% myocytes, 27% fibroblasts, 7% endothelial cells, and 10% vascular smooth muscle cells. Moreover, our morphometric and FACS data demonstrated similar percentages in the three regions examined. During murine neonatal cardiac development, we observed a marked increase in numbers of cardiac fibroblasts and a resultant decrease in percentages of myocytes in late neonatal development (day 15). Finally, FACS analyses of the rat heart during development displayed similar results in relation to increases in cardiac fibroblasts during development; however, cell populations in the rat differed markedly from those observed in the mouse. Taken together, these data enabled us to establish a homeostatic model for the myocardium that can be compared with genetic and cardiac disease models.

cardiac fibroblast; myocyte; cardiac remodeling; flow cytometry

This article has been cited by other articles:

				I. Banerjee, J. W. Fuseler, A. R. Intwala, and T. A. Baudino IL-6 loss causes ventricular dysfunction, fibrosis, reduced capillary density, and dramatically alters the cell populations of the developing and adult heart Am J Physiol Heart Circ Physiol, May 1, 2009; 296(5): H1694 - H1704. [Abstract] [Full Text] [PDF]

				N. Gellings Lowe, J. S. Swaney, K. M. Moreno, and R. A. Sabbadini Sphingosine-1-phosphate and sphingosine kinase are critical for transforming growth factor-{beta}-stimulated collagen production by cardiac fibroblasts Cardiovasc Res, May 1, 2009; 82(2): 303 - 312. [Abstract] [Full Text] [PDF]

				A. M. Manso, S.-M. Kang, S. V. Plotnikov, I. Thievessen, J. Oh, H. E. Beggs, and R. S. Ross Cardiac fibroblasts require focal adhesion kinase for normal proliferation and migration Am J Physiol Heart Circ Physiol, March 1, 2009; 296(3): H627 - H638. [Abstract] [Full Text] [PDF]