AJP - Heart AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Am J Physiol Heart Circ Physiol 293: H1883-H1891, 2007. First published June 29, 2007; doi:10.1152/ajpheart.00514.2007
0363-6135/07 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
293/3/H1883    most recent
00514.2007v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Banerjee, I.
Right arrow Articles by Baudino, T. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Banerjee, I.
Right arrow Articles by Baudino, T. A.

Determination of cell types and numbers during cardiac development in the neonatal and adult rat and mouse

Indroneal Banerjee, John W. Fuseler, Robert L. Price, Thomas K. Borg, and Troy A. Baudino

Cell and Developmental Biology and Anatomy, University of South Carolina School of Medicine, Columbia, South Carolina

Submitted 30 April 2007 ; accepted in final form 28 June 2007

Cardiac fibroblasts, myocytes, endothelial cells, and vascular smooth muscle cells are the major cellular constituents of the heart. The aim of this study was to observe alterations in myocardial cell populations during early neonatal development in the adult animal and to observe any variations of the cardiac cell populations in different species, specifically, the rat and mouse. Whole hearts were isolated from either mice or rats during the neonatal and adult stages of development, and single cell suspensions were prepared via sequential collagenase digestion. Heterogeneous cell populations were immunolabeled for specific cell types and analyzed using fluorescence-activated cell sorting (FACS). In addition, the left ventricle, right ventricle, and septa were isolated, fixed, and sectioned for morphometric analyses. These same cardiac regions were also analyzed using FACS. We observed that the adult murine myocardium is composed of ~56% myocytes, 27% fibroblasts, 7% endothelial cells, and 10% vascular smooth muscle cells. Moreover, our morphometric and FACS data demonstrated similar percentages in the three regions examined. During murine neonatal cardiac development, we observed a marked increase in numbers of cardiac fibroblasts and a resultant decrease in percentages of myocytes in late neonatal development (day 15). Finally, FACS analyses of the rat heart during development displayed similar results in relation to increases in cardiac fibroblasts during development; however, cell populations in the rat differed markedly from those observed in the mouse. Taken together, these data enabled us to establish a homeostatic model for the myocardium that can be compared with genetic and cardiac disease models.

cardiac fibroblast; myocyte; cardiac remodeling; flow cytometry



Address for reprint requests and other correspondence: T. A. Baudino, Univ. of South Carolina School of Medicine, 6439 Garners Ferry Rd., Columbia, SC 29209 (e-mail: tbaudino{at}med.sc.edu)




This article has been cited by other articles:


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
I. Banerjee, J. W. Fuseler, A. R. Intwala, and T. A. Baudino
IL-6 loss causes ventricular dysfunction, fibrosis, reduced capillary density, and dramatically alters the cell populations of the developing and adult heart
Am J Physiol Heart Circ Physiol, May 1, 2009; 296(5): H1694 - H1704.
[Abstract] [Full Text] [PDF]


Home page
Cardiovasc ResHome page
N. Gellings Lowe, J. S. Swaney, K. M. Moreno, and R. A. Sabbadini
Sphingosine-1-phosphate and sphingosine kinase are critical for transforming growth factor-{beta}-stimulated collagen production by cardiac fibroblasts
Cardiovasc Res, May 1, 2009; 82(2): 303 - 312.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
A. M. Manso, S.-M. Kang, S. V. Plotnikov, I. Thievessen, J. Oh, H. E. Beggs, and R. S. Ross
Cardiac fibroblasts require focal adhesion kinase for normal proliferation and migration
Am J Physiol Heart Circ Physiol, March 1, 2009; 296(3): H627 - H638.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2007 by the American Physiological Society.