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Am J Physiol Heart Circ Physiol 295: H533-H542, 2008. First published June 6, 2008; doi:10.1152/ajpheart.00094.2008
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Bone marrow-derived stromal cells home to and remain in the infarcted rat heart but fail to improve function: an in vivo cine-MRI study

¹Cardiac Metabolism Research Group, Department of Physiology, Anatomy and Genetics, and ²Nuffield Department of Clinical Laboratory Sciences, University of Oxford; ³Stem Cell Research Laboratory, National Blood Service, Oxford Centre, John Radcliffe Hospital; and ⁴British Heart Foundation Experimental Magnetic Resonance Unit, Wellcome Trust Centre for Human Genetics, Oxford; and ⁵National Heart and Lung Institute, Imperial College School of Science, Technology and Medicine, London, United Kingdom

Submitted 28 January 2008 ; accepted in final form 3 June 2008

Basic and clinical studies have shown that bone marrow cell therapy can improve cardiac function following infarction. In experimental animals, reported stem cell-mediated changes range from no measurable improvement to the complete restoration of function. In the clinic, however, the average improvement in left ventricular ejection fraction is around 2% to 3%. A possible explanation for the discrepancy between basic and clinical results is that few basic studies have used the magnetic resonance (MR) imaging (MRI) methods that were used in clinical trials for measuring cardiac function. Consequently, we employed cine-MR to determine the effect of bone marrow stromal cells (BMSCs) on cardiac function in rats. Cultured rat BMSCs were characterized using flow cytometry and labeled with iron oxide particles and a fluorescent marker to allow in vivo cell tracking and ex vivo cell identification, respectively. Neither label affected in vitro cell proliferation or differentiation. Rat hearts were infarcted, and BMSCs or control media were injected into the infarct periphery (n = 34) or infused systemically (n = 30). MRI was used to measure cardiac morphology and function and to determine cell distribution for 10 wk after infarction and cell therapy. In vivo MRI, histology, and cell reisolation confirmed successful BMSC delivery and retention within the myocardium throughout the experiment. However, no significant improvement in any measure of cardiac function was observed at any time. We conclude that cultured BMSCs are not the optimal cell population to treat the infarcted heart.

cardiac function; magnetic resonance imaging; cell tracking

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