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This Article Full Text Full Text (PDF) All Versions of this Article: 295/4/H1788    most recent 253.2008v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Kaul, D. K. Articles by Yedgar, S. PubMed PubMed Citation Articles by Kaul, D. K. Articles by Yedgar, S.

Additive effect of red blood cell rigidity and adherence to endothelial cells in inducing vascular resistance

¹Hematology Division, Albert Einstein College of Medicine, Bronx, New York; ²Department of Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel; and ³Institute of Bioengineering, Department Cell Biophysics, Aachen University of Applied Sciences, Juelich, Germany

Submitted 11 March 2008 ; accepted in final form 20 August 2008

To explore the contribution of red blood cell (RBC) deformability and interaction with endothelial cells (ECs) to circulatory disorders, these RBC properties were modified by treatment with hydrogen peroxide (H₂O₂), and their effects on vascular resistance were monitored following their infusion into rat mesocecum vasculature. Treatment with 0.5 mM H₂O₂ increased RBC/EC adherence without significant alteration of RBC deformability. At 5.0 mM H₂O₂, RBC deformability was considerably reduced, inducing a threefold increase in the number of undeformable cells, whereas RBC/EC adherence was not further affected by the increased H₂O₂ concentration. This enabled the selective manipulation of RBC adherence and deformability and the testing of their differential effect on vascular resistance. Perfusion of RBCs with enhanced adherence and unchanged deformability (treatment with 0.5 mM H₂O₂) increased vascular resistance by about 35% compared with untreated control RBCs. Perfusion of 5.0 mM H₂O₂-treated RBCs, with reduced deformability (without additional increase of adherence), further increased vascular resistance by about 60% compared with untreated control RBCs. These results demonstrate the specific effects of elevated adherence and reduced deformability of oxidized RBCs on vascular resistance. These effects can be additive, depending on the oxidation conditions. The oxidation-induced changes applied in this study are moderate compared with those observed in RBCs in pathological states. Yet, they caused a considerable increase in vascular resistance, thus demonstrating the potency of RBC/EC adherence and RBC deformability in determining resistance to blood flow in vivo.

erythrocyte rheology; aggregability; adhesion; circulatory disorders