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This Article Full Text Full Text (PDF) All Versions of this Article: 296/4/H976    most recent 01134.2008v2 01134.2008v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Zhao, T. Articles by Wang, Y. Search for Related Content PubMed PubMed Citation Articles by Zhao, T. Articles by Wang, Y.

Stem cell homing and angiomyogenesis in transplanted hearts are enhanced by combined intramyocardial SDF-1 delivery and endogenous cytokine signaling

Departments of ¹Pathology and Laboratory Medicine and ²Pharmacology and Cell Biophysics, University of Cincinnati Medical Center, Cincinnati, Ohio; and ³Qinghai Red Cross Hospital, Xining, Qinghai, People's Republic of China

Submitted 27 October 2008 ; accepted in final form 20 January 2009

We used a heterotopic transplanted working heart model to probe the collaborative role of bone marrow-derived progenitor cells (BPCs) and stromal cell-derived factor (SDF)-1 in attenuating tissue remodeling in recipient and transplanted hearts. BPCs from male transgenic rats expressing green fluorescent protein (GFP⁺ BPCs, 2 x 10⁶ cells) were injected intravenously into myeloablated female rats. One month later, heterotopic heart transplantation was performed. The left anterior descending coronary artery (LAD) of the recipient heart was occluded permanently. Mesenchymal stem cells (MSCs; 2 x 10⁶ cells) with a null gene (null group) or overexpressing SDF-1 (SDF-1 group) were injected intramyocardially in the LAD perfusion region of both recipient and transplanted hearts. Recipient and transplanted hearts (n = 10 hearts/group) were harvested 21 days later for analysis. The survival of transplanted hearts was assessed daily by palpation in additional animals (n = 7). Five days after LAD occlusion, subpopulations of GFP⁺ BPCs in the circulation were significantly higher in the SDF-1 group. Y chromosome, 5-bromo-2'-deoxyuridine, Ki67-positive nuclei, newly formed vessels, and GFP⁺ cells significantly increased in transplanted hearts of the SDF-1 group at 21 days after the injection of MSCs overexpressing SDF-1, whereas fewer TUNEL-positive nuclei were found. The survival of transplanted hearts was also markedly increased in the SDF-1 group (P < 0.05). Supplementation of endogenous cytokines released from the ischemic myocardium with exogenous MSCs overexpressing SDF-1 significantly increased BPC homing to acutely ischemic recipient and progressively ischemic transplanted hearts. BPC recruitment resulted in the regeneration of new cardiomyocytes and blood vessels and extended survival of the transplanted hearts.

stromal cell-derived factor-1; heterotopic heart transplantation; stem cells; cell migration

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