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This Article Full Text Full Text (PDF) All Versions of this Article: 296/5/H1434    most recent 00993.2008v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Schwartz, M. K Articles by Miller, V. M Search for Related Content PubMed PubMed Citation Articles by Schwartz, M. K Articles by Miller, V. M

Systemic injection of planktonic forms of mammalian-derived nanoparticles alters arterial response to injury in rabbits

¹Department of Physiology and Biomedical Engineering, ²Division of Nephrology, Department of Internal Medicine, and ³Department of Surgery, Mayo Clinic College of Medicine, Rochester, Minnesota

Submitted 12 September 2008 ; accepted in final form 6 March 2009

Experiments were designed to test the hypothesis that the systemic delivery of planktonic forms of nanoparticles (NPs) derived from calcified, diseased human tissue or bovine blood are transmissible particles that exacerbate arterial response to injury. New Zealand White rabbits in which the endothelium was mechanically removed from one carotid artery were injected intravenously with either saline (control), lipopolysaccharide (LPS; surrogate for subclinical infection), hydroxyapatite crystals (HA; surrogate for NP shell), HA crystals exposed to culture media, or planktonic forms of bovine- or human-derived NPs. Carotid arteries were monitored by ultrasonography for 5 wk and then removed for histological examination. Uninjured arteries from all animals in each group remained patent with a normal anatomy. Injured arteries from 6 of 11 animals injected with human-derived NPs occluded and/or calcified; none of the injured arteries from animals in the other groups occluded (n = 28; P 0.05). Injured arteries of rabbits injected with LPS or HA crystals developed eccentric hyperplasia. Discontinuous internal elastic laminae and thinning media characterized arteries from animals injected with bovine-derived NPs or cultured HA crystals. In conclusion, the systemic administration of planktonic forms of human-derived NPs exacerbated arterial response to injury distinct from that of bovine-derived NPs and other inflammatory agents.

inflammation; intimal hyperplasia; carotid artery