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This Article Full Text Full Text (PDF) All Versions of this Article: 296/5/H1586    most recent 00867.2008v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Conklin, D. J. Articles by Bhatnagar, A. Search for Related Content PubMed PubMed Citation Articles by Conklin, D. J. Articles by Bhatnagar, A.

Glutathione-S-transferase P protects against endothelial dysfunction induced by exposure to tobacco smoke

¹Institute of Molecular Cardiology and ²Department of Biochemistry and Molecular Biology, University of Louisville, Louisville, Kentucky

Submitted 6 August 2008 ; accepted in final form 2 March 2009

Exposure to tobacco smoke impairs endothelium-dependent arterial dilation. Reactive constituents of cigarette smoke are metabolized and detoxified by glutathione-S-transferases (GSTs). Although polymorphisms in GST genes are associated with the risk of cancer in smokers, the role of these enzymes in regulating the cardiovascular effects of smoking has not been studied. The P isoform of GST (GSTP), which catalyzes the conjugation of electrophilic molecules in cigarette smoke such as acrolein, was expressed in high abundance in the mouse lung and aorta. Exposure to tobacco smoke for 3 days (5 h/day) decreased total plasma protein. These changes were exaggerated in GSTP^–/– mice. Aortic rings isolated from tobacco smoke-exposed GSTP^–/– mice showed greater attenuation of ACh-evoked relaxation than those from GSTP^+/+ mice. The lung, plasma, and aorta of mice exposed to tobacco smoke or acrolein (for 5 h) accumulated more acrolein-adducted proteins than those tissues of mice exposed to air, indicating that exposure to tobacco smoke results in the systemic delivery of acrolein. Relative to GSTP^+/+ mice, modification of some proteins by acrolein was increased in the aorta of GSTP^–/– mice. Aortic rings prepared from GSTP^–/– mice that inhaled acrolein (1 ppm, 5 h/day for 3 days) or those exposed to acrolein in an organ bath showed diminished ACh-induced arterial relaxation more strongly than GSTP^+/+ mice. Acrolein-induced endothelial dysfunction was prevented by pretreatment of the aorta with N-acetylcysteine. These results indicate that GSTP protects against the endothelial dysfunction induced by tobacco smoke exposure and that this protection may be related to the detoxification of acrolein or other related cigarette smoke constituents.

acrolein; aldehydes; environmental cardiology; oxidative stress

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