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This Article Full Text Full Text (PDF) Supplemental Video Buy All Versions of this Article: 297/6/H2054    most recent 00836.2009v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Bohl, S. Articles by Lygate, C. A. PubMed PubMed Citation Articles by Bohl, S. Articles by Lygate, C. A.

Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart

¹Department of Cardiovascular Medicine, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom; and ²Franz-Volhard-Klinik, Helios-Klinikum Berlin, Cardiology, Charité University Medicine Berlin, Berlin, Germany

Submitted September 2, 2009 ; accepted in final form October 5, 2009

Cardiac ischemia-reperfusion experiments in the mouse are important in vivo models of human disease. Infarct size is a particularly important scientific readout as virtually all cardiocirculatory pathways are affected by it. Therefore, such measurements must be exact and valid. The histological analysis, however, remains technically challenging, and the resulting quality is often unsatisfactory. For this report we have scrutinized each step involved in standard double-staining histology. We have tested published approaches and challenged their practicality. As a result, we propose an improved and streamlined protocol, which consistently yields high-quality histology, thereby minimizing experimental noise and group sizes.

tetrazolium chloride staining; methodology; dual-dye technique; reperfused myocardial infarction