Supplemental Video

Two supplemental video files.

Files in this Data Supplement:

• Movie 1 - UBM cineloop showing the umbilical and uteroplacental circulations at E14.5 obtained non-invasively from an isoflurane-anesthetized pregnant mouse. Echoes arising from the beating embryonic heart are visible within the embryo on the left and the placenta is visible beneath the maternal skin on the top right. The umbilical cord crosses the amniotic cavity next to a cross-section through an embryonic limb to enter the placenta on the right. The highly echogenic (i.e. bright) embryonic blood can be seen moving within the umbilical cord. Within the placenta, the relatively echolucent maternal blood can be seen moving within the maternal arterial canal and into an arterial canal branch. Periodic movement of the entire image is caused by maternal breathing. Small divisions on the scale bars are 100 µm.
• Movie 2 - UBM cineloop showing the embryo and vitelline and umbilical circulations at E9.5 obtained non-invasively from an isoflurane-anesthetized pregnant mouse. The embryo is shown within the amniotic cavity within a cross-section of the uterus imaged through the maternal skin. Echoes arising from the beating U-shaped embryonic heart are visible within the embryo. The head of the embryo is on the left, and the tail region is beneath the beating heart. Relatively echogenic (i.e. bright) embryonic blood can be seen moving within the vitelline and umbilical vessels crossing the amniotic cavity. The vitelline vessels (upper right of embryo) perfuse the yolk sac that encircles the embryo, and the umbilical vessels (lower right of embryo) perfuse the developing chorioallantoic placenta. Periodic movement of the entire image is caused by maternal breathing. Small divisions on the scale bars are 100 µm.