Supplemental Videos

Eleven Quicktime videos in .MOV format, supplementing Figures 2, 3, and 5 in the article.

Files in this Data Supplement:

• Figure 2, Video 1 - This representative video depicts several Oregon Green 488 labeled T lymphocytes moving through the MLN HEV, and corresponds to Figure 2A in the printed manuscript. The HEV can be identified by the red transfluospheres indicating blood flow (see text).
• Figure 2, Video 2 - This representative video depicts several fluorescently labeled inert 3 μm polymer beads moving through the MLN HEV, and corresponds to Figure 2B in the manuscript. The HEV can be identified by the red transfluospheres indicating blood flow (see text).
• Figure 3, Video 1 - This representative video shows the movement of Oregon Green 488 labeled T lymphocytes in the spleen. The white pulp can be identified by the blood flow, which is marked by the red transfluospheres. Both interacting and non-interacting cells can be seen in this video, which corresponds to Figure 3A in the manuscript.
• Figure 3, Video 2 - This representative video shows the movement of fluorescently labeled inert 3 μm polymer beads in the spleen. The white pulp can be identified by the blood flow, which is marked by the red transfluospheres. Note the similarity between bead and T cell movement. This video corresponds to Figure 3B in the manuscript.
• Figure 5, Video 1 - The visualization of terminal vessels, buds, and honeycombs can be seen in this representative video, which corresponds to Figure 5A. Blood flow is identified by 150kD FITC-dextran.
• Figure 5, Video 2 - Pulsating vessels can be seen in this representative video, which corresponds with Figure 5A(d). The pulsating vessels appear to lead into terminal vessels, which end in the honeycomb or bud region of the white pulp.
• Figure 5, Video 3 - Representative video of the TIE2-GFP spleen with injection of 0.04 μm transfluospheres. The transfluospheres (red) can be seen to fill the honeycomb areas of the white pulp at the ends of some, but not all of the TEK (TIE2) expressing endothelial lined vessels (green).
• Figure 5, Video 4 - Using the same mouse as was shown in the previous video, these images verify that the transfluospheres are indeed marking blood flow, as identified by the injection of FITC-dextran. A higher power view of the honeycomb region is also shown in this video which corresponds with Figure 5B.
• Figure 5, Video 5 - Oregon Green 488 labeled T lymphocytes firmly adhere in the white pulp of the spleen beyond the ends of the TEK expressing endothelial lined vessels. This representative video corresponds to Figure 5C(a), and shows Oregon Green 488 labeled T lymphocytes in the TIE2-GFP murine spleen, with transfluospheres identifying the white pulp.
• Figure 5, Video 6 - 3 μm polymer beads are also capable of adhering within the white pulp; however, as seen in this representative video, the beads tend to adhere just beyond the ends of the TEK expressing endothelial lined vessels. This video corresponds with Figure 5C(b).
• Figure 5, Video 7 - This representative video shows an Oregon Green 488 labeled T lymphocyte moving through one of the channels in white pulp. The first part of this video is in real-time and then the video clip is repeated at half-speed, in order to allow better visualization of the cell moving through the channel. The blood flow is marked by red transfluospheres. This video corresponds with Figure 5D.