AJP - Heart AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 281: H1353-H1363, 2001;
0363-6135/01 $5.00
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lindauer, U.
Right arrow Articles by Dirnagl, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lindauer, U.
Right arrow Articles by Dirnagl, U.
Vol. 281, Issue 3, H1353-H1363, September 2001

Nitric oxide from perivascular nerves modulates cerebral arterial pH reactivity

Ute Lindauer*, Alexander Kunz*, Sigrid Schuh-Hofer, Johannes Vogt, Jens P. Dreier, and Ulrich Dirnagl

Department of Experimental Neurology, Humboldt University, Charité Hospital, 10098 Berlin, Germany


    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

In the isolated rat middle cerebral artery (MCA) we investigated the role of nitric oxide (NO)/cGMP in the vasodilatory response to extraluminal acidosis. Acidosis increased vessel diameter from 140 ± 27 µm (pH 7.4) to 187 ± 30 µm (pH 7.0, P < 0.01). NO synthase (NOS) inhibition by Nomega -nitro-L-arginine (L-NNA, 10 µM) reduced baseline diameter (103 ± 20 µm, P < 0.01) and attenuated response to acidosis (9 ± 8 µm). Application of the NO-donors 3-morpholinosydnonimine (1 µM) or S-nitroso-N-acetylpenicillamine (1 µM), or of 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP, 100 µM) reestablished pre-L-NNA diameter at pH 7.4 and reversed L-NNA-induced attenuation of the vessel response to acidosis. Restoration of pre-L-NNA diameter (pH 7.4) by papaverine (20 µM) or nimodipine (30 nM) had no effect on the attenuated response to acidosis. Guanylyl cyclase inhibition with 1H-[1,2,4]oxadiazolo[4,3-a]-quinoxalin-1-one (5 µM) or NOS-inhibition with 7-nitroindazole (7-NI, 100 µM) reduced baseline vessel diameter (109 ± 8 or 127 ± 11 µm, respectively) and vasodilation to acidosis, and restoration of baseline diameter with 8-BrcGMP (30 µM) completely restored dilation to pH 7.0. Chronic denervation of NOS-containing perivascular nerves in vivo 14 days before artery isolation significantly reduced pH-dependent reactivity in vitro (diameter increase sham: 48 ± 14 µm, denervated: 14 ± 8 µm), and 8-BrcGMP (30 µM) restored dilation to pH 7.0 (denervated: 49 ± 31 µm). Removal of the endothelium did not change vasodilation to acidosis. We conclude that NO, produced by neuronal NOS of perivascular nerves, is a modulator in the pH-dependent vasoreactivity.

acidosis; endothelium; isolated cerebral artery; nitric oxide synthase; rat; soluble guanylyl cyclase


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

THE BIORADICAL NITRIC OXIDE (NO) has been shown to be not only a potent vasodilator, but also an important modulator of cerebrovascular reactivity (20, 26). A basal NO level in perivascular brain tissue seems to be important for proper smooth muscle cell function of cerebral vessels. Until now it is not clear which type of cells are responsible for the modulatory level of NO. Besides cerebrovascular endothelial cells, constitutive NO synthase (NOS) [type I, neuronal (nNOS) and type III, endothelial (eNOS)] can be found in astrocytes and neurons (8, 17). Whether endothelial, astrocytic, or neuronal NO production accounts for basal NO concentration in the tissue remains unclear. For the cerebrovascular response to hypercapnia or tissue acidosis, NO dependency has been shown in both in vivo and in vitro studies (10, 30, 43). During hypercapnia, the role of NO in vivo is modulatory (20), and no acidosis-induced increase in NO or cGMP production has been shown in vitro (43). There is some evidence from the literature that endothelial function as well as NO originating from vascular eNOS is not essential for hypercapnic vasodilation (42, 43).

The investigation of the source of NO and of the cellular compartment responsible for the basal NO level in the cerebral tissue within the cerebrovascular system is still of great importance. Many efforts are undertaken to increase this basal NO level to improve vascular reactivity under pathophysiological conditions. The most promising experimental approaches are the pharmacological induction of eNOS activity by 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors [statins (13)] or the increase of eNOS activity by gene transfer (e.g., Ref. 2). However, to thoroughly assess the functional consequences of increased eNOS activity, we have at first to improve our knowledge on the specific role of eNOS and nNOS in cerebrovascular reactivity under physiological conditions.

The present study is the first to investigate the role of eNOS and nNOS in the NOS-dependent vasodilation to acidosis, using both pharmacological as well as nonpharmacological approaches in the same experimental setup. We examined whether NO originating from endothelial cells or perivascular nerves accounts for basal NO levels in isolated cerebral arteries and smooth muscle reactivity to extraluminal acidosis. In addition to pharmacological enzyme inhibition strategies, vascular endothelium was removed by intraluminal detergent or air application, and perivascular nerve degeneration was induced by in vivo chronic dissection of the nasociliary nerve (NCN) and parasympathetic fibers (PSF) originating from the sphenopalatine ganglion 14 days before isolation of the arteries.


    MATERIALS AND METHODS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Male Wistar rats (240-260 g) were anesthetized with halothane and then decapitated. The brain was rapidly removed from the skull and placed in cold (4°C) 3-(N-morpholino)propanesulfonic acid (MOPS)-buffered saline solution (with 1% dialyzed bovine serum albumin) containing (in mM) 144 NaCl, 3.0 KCl, 2.5 CaCl2, 1.5 MgSO4, 1.21 NaH2PO4, 0.02 EDTA, 2.0 pyruvate, 2.0 MOPS, and 5.0 glucose (11), pH 7.40 ± 0.02. Approximately 1 cm of the middle cerebral artery (MCA) was carefully dissected from the brain surface beginning at the circle of Willis. The surrounding parenchymal tissue was carefully removed, leaving the perivascular nerve endings within the adventitial layer intact as shown for NOS-positive fibers in Fig. 7A. The MCA was transferred into an organ chamber (arteriograph, Living Systems; Burlington, VT) containing cold (4°C) MOPS-buffered saline solution (with 1% dialyzed bovine serum albumin, as described above). Glass pipettes were inserted into each end of the artery, and a segment of the artery without branches, ~1 mm in length, was placed between the micropipettes. Two 10-0 nylon threads were used on each side to fix the MCA on the glass pipettes. The vessel was continuously perfused with an intraluminal flow of 100 µl/min of MOPS-buffered saline solution containing 1% bovine serum albumin (37°C) at a transmural pressure of 80 mmHg due to raised in- and outflow reservoirs. The extraluminal bath was then switched to MOPS-buffered saline solution without bovine serum albumin, heated to 37°C, and continuously exchanged at a rate of 20 ml/min (22).

The vessel chamber was placed on an inverted microscope equipped with a video camera and monitor for online analysis of luminal diameters of the arteries. The camera was connected to a personal computer to store video images after each manipulation for later off-line analysis.

Before the experiments were started, the vessels were allowed to equilibrate for 1 h. Within this time, spontaneous tone developed, reducing resting diameter to ~80% compared with diameters measured directly after pressurizing. During the entire experiment, temperature of the extraluminal bath, perfusion inflow pressure, and flow rate were monitored and kept constant.

Acetylcholine was applied intraluminally by adding the drug into the reservoir of the inflow pipette; all other pharmacologically active substances were added to the reservoir of the extraluminal bath. Because of continuous circulation of the buffer solution, drug concentrations changed slowly in the organ chamber and needed 5-10 min to equilibrate.

Acidotic vasodilation was induced by changing the pH of the extraluminal bath to 7.00 ± 0.03 by adding HCl to the MOPS-buffered saline solution in the reservoir. Luminal diameters were registered after an equilibration period of 15 min. By changing the extraluminal bath to buffer solution with physiological pH values, vessels were allowed to return to baseline diameters.

Experimental Design

Studies I-V. The modulatory role of NO in the isolated cerebral artery was studied, which included the influence of NOS inhibition and subsequent vasodilator application (NO donors, cGMP, NO-independent vasodilators) on vessel response to extraluminal acidosis (see Fig. 1).


View larger version (34K):
[in this window]
[in a new window]
  		Fig. 1.   Experimental paradigm. L-NNA, Nomega -nitro-L-arginine; SIN-1, 3-morpholinosyndnonimine; SNAP, S-nitroso-N-acetylpenicillamine; ODQ, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one; 7-NI, 7-nitroindazole; PSF, parasympathetic fibers; NCN, nasociliary nerve; CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate.

After a baseline response to acidosis was recorded, unspecific NOS inhibition was induced by adding 10 µM Nomega -nitro-L-arginine (L-NNA, Sigma) to the extraluminal bath for 30 min. The vessel response to acidosis was tested again. In studies I (n = 5) and II (n = 4), the NO donors 3-morpholinosydnonimine (SIN-1, 1 µM in MOPS buffer solution containing 10 µM L-NNA, Research Biochemicals International) or S-nitroso-N-acetylpenicillamine (SNAP, 1 µM in MOPS buffer solution containing 10 µM L-NNA, Research Biochemicals International), respectively, were then added to the extraluminal bath solution for 10 to 15 min to restore resting baseline diameter to pre-L-NNA levels. The response to extraluminal acidosis was tested again. In studies III (n = 4), IV (n = 3), and V (n = 3), the cell-permeable cGMP analog 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP, 100 µM in MOPS buffer solution containing 10 µM L-NNA; Sigma), the NO-independent vasodilators papaverine (20 µM in MOPS buffer solution containing 10 µM L-NNA; Sigma), or nimodipine (30 nM in MOPS buffer solution containing 10 µM L-NNA; Sigma), respectively, were used instead of the NO donors, SIN-1 or SNAP, to reestablish the pre-L-NNA resting vessel diameter.

Study VI. The modulatory role of cGMP in the isolated cerebral artery was studied, which included the influence of guanylyl cyclase inhibition and subsequent cGMP application on vessel response to extraluminal acidosis. After a baseline response to acidosis was recorded, soluble guanylyl cyclase inhibition was induced by adding 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) to the extraluminal bath for 30 min (5 µM ODQ dissolved in ethanol with a final concentration of 0.1% ethanol in MOPS buffer solution; Research Biochemicals International). The vessel response to acidosis was tested again (n = 6). In two of these arteries, the cell-permeable cGMP analog 8-BrcGMP (30 µM in MOPS buffer solution containing 5 µM ODQ; Sigma) was then added to the extraluminal bath solution for 10-15 min to restore resting baseline diameter to ODQ levels. The response to extraluminal acidosis was tested again.

Studies VII-X. The role of different NOS isoforms (neuronal vs. endothelial) was studied by testing the influence of inhibition of NOS via 7-nitroindazole (7-NI) application or chronic degeneration of perivascular nerve fibers (NCN and PSF) or of acute removal of vascular endothelium on vessel response to extraluminal acidosis.

Studies VII and VIII. We investigated the contribution of 7-NI inhibitable NOS or of nNOS, mainly located in perivascular nerve fibers in the vessel wall (17), on basal NO levels in the isolated artery. After a baseline response to acidosis was recorded in study VII (n = 8), NOS inhibition was induced by adding 7-NI (100 µM, dissolved in ethanol, with a final concentration of 0.25% ethanol in MOPS buffer solution; Sigma) to the extraluminal bath for 15 min. After the vessel response to acidosis was tested, 8-BrcGMP (30 µM in MOPS buffer solution containing 100 µM 7-NI; Sigma) was added to the extraluminal bath solution for 15 min to restore resting baseline diameter to pre-7-NI levels. The response to extraluminal acidosis was tested again. In two additional arteries, the effect of up to 0.25% ethanol in MOPS buffer solution to extraluminal acidosis was tested, and no effect of the vehicle ethanol on the acidotic vasodilation response was observed (vehicle control to studies VI and VII, data not shown).

The specificity of the NOS inhibitor 7-NI in in vitro as well as in vivo experiments has been discussed controversially (14, 27). Therefore, we tested the effect of 7-NI on the endothelium-dependent vasodilation to acetylcholine (100 µM in MOPS buffer solution; Sigma) in another four arteries.

Study VIII. nNOS-containing perivascular nerve fibers at the anterior portion of the circle of Willis and its branches including the MCA are mainly originating from the NCN and PSF from the sphenopalatine ganglion (7, 17, 36). Extracranial transsection of these nerve bundles at the ethmoidal foramen leads to a complete loss of nNOS-containing perivascular nerve fibers within 2 wk as shown by NADPH-diaphorase histochemistry (36). Therefore, in study VIIIb (n = 10), 2 wk before the isolation of the MCA, the NCN and PSF were transsected according to the method described previously by Suzuki et al. (36). In brief, rats (200-220 g) were anesthetized with chloral hydrate (5%, 200 mg/kg body wt ip). The ethmoidal foramen at the nasal orbital wall ipsilaterally to the later isolated MCA was carefully prepared, and the NCN and PSF were thoroughly transsected. A sham-operated group (study VIIIa, n = 8) served as a control. Responses of isolated ipsilateral MCAs to extraluminal acidosis (n = 10 denervated group, n = 8 sham group) as well as to 20 mM potassium (extraluminal) and 100 µM acetylcholine (intraluminal) (n = 4 denervated group, n = 3 sham group) were tested 12 to 16 days after nerve transsection. In four arteries of the denervated group, the effect of 8-BrcGMP (30 µM in MOPS buffer solution; Sigma) on the response to extraluminal acidosis was tested. NADPH diaphorase histochemistry was processed according to the protocol of Tomimoto et al. (39) to confirm degeneration of nNOS-containing perivascular nerve fibers in the transsection group compared with preserved NOS staining in the sham-operated group. In brief, additional animals (n = 2 each) under deep pentobarbital anesthesia (100 mg/kg) were perfusion fixed with 2% paraformaldehyde and 0.1% picric acid in 20 mM phosphate-buffered saline at 4°C. The brain was removed and postfixed (2% paraformaldehyde and 0.1% picric acid in 20 mM phosphate-buffered saline) for 2 h. The MCA was carefully removed from the pial surface and stored in 20 mM phosphate-buffered saline containing 20% sucrose at 4°C for 24 h. Subsequently the isolated artery was incubated in 20 mM phosphate-buffered saline containing 0.3% Triton X-100, 1 mg/ml NADPH, and 0.25 mg/ml nitroblue tetrazolium at 37°C for 45 min. The vessels were mounted on slides, coverslipped, and investigated under a conventional microscope.

Studies IX and X. To investigate the contribution of eNOS in the vasodilatory response of the isolated MCA to extraluminal acidosis, the endothelium was removed. After testing baseline vasodilation to intraluminal application of acetylcholine (100 µM in MOPS buffer solution; Sigma) to verify the integrity of endothelium-dependent vasoreactivity, the baseline response to acidosis was recorded. In study IX (n = 6), the detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS, 0.3% in intraluminal buffer solution; Sigma) was then slowly perfused intraluminally for 2.5 min. In study X (n = 5), 8 ml of air was passed through the lumen of the MCA over a period of 15 min with the intraluminal pressure never exceeding 80 mmHg. In both study groups, removal of the endothelium was confirmed by the absence of vasodilation during intraluminal application of acetylcholine. The response to extraluminal acidosis was tested again.

Data Analysis

Values are means ± SD. Comparisons were made using ANOVA, followed by multiple comparison procedures (Bonferroni's method). In studies VIIIa and VIIIb, comparison was made using unpaired Student's t-test (SigmaStat, Jandel). P values <0.05 were considered statistically significant.


    RESULTS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

After spontaneous tone was developed (20% reduction of resting diameter compared with diameters measured directly after pressurizing) within the 1-h equilibration period after preparation, smooth muscle cell function was tested at the beginning of each experiment by increasing extraluminal potassium concentration to 20 mM. Only vessels were used for the experiments, which showed a potassium-induced dilation of more than 25%.

Physiological Response

After equilibration, MCA diameter stabilized at 140.0 ± 26.8 µm at physiological pH values (pH 7.4), and extraluminal pH reduction to 7.0 led to a consistent and statistically significant (P < 0.01) diameter increase of 46.8 ± 21.7 µm (35.1 ± 18.5%), reaching vessel diameters of 186.8 ± 30.2 µm (n = 44).

Unspecific NOS Inhibition and Subsequent Vasodilator Application

Unspecific NOS inhibition with L-NNA reduced vessel diameters at physiological extraluminal buffer solutions to ~74% and reduced acidotic vasodilation to a diameter increase of only 8.7 ± 8.4 µm (from 102.7 ± 20.5 µm at pH 7.4 to 110.9 ± 26.4 µm at 7.0) (P < 0.01). Vasodilatory reaction to acidosis was, therefore, still present (P < 0.01) but markedly reduced to ~8% during NOS inhibition. Whereas we reestablished vessel diameter at pH 7.4 by adding the NO donors SIN-1 or SNAP or the cell-permeable cGMP analog 8-BrcGMP extraluminally during continued NOS inhibition, the vessel reactivity to acidosis was restored. In contrast, when MCA diameter was increased to pre-L-NNA values by adding the appropriate amount of the NO-independent vasodilators papaverine or nimodipine, the vasoreactivity to acidosis remained significantly reduced (diameter increase at pH 7.0 of only 3.7 ± 6.4 µm with papaverine or 9.0 ± 5.1 µm with nimodipine) (Fig. 2).


View larger version (53K):
[in this window]
[in a new window]
  		Fig. 2.   Effect of extraluminal application of the nitric oxide (NO) donors SIN-1 (1 µM, study I), or SNAP (1 µM, study II), or the cell-permeable cGMP analog 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP, 100 µM, study III), the NO-independent vasodilators papaverine (20 µM, study IV), or nimodipine (30 nM, study V) on L-NNA (10 µM)-induced attenuation of resting middle cerebral artery (MCA) diameter at pH 7.4 and vasodilator response to extraluminal acidosis (pH 7.0). NO synthase (NOS) inhibition by L-NNA reduced resting diameter at pH 7.4 as well as vessel diameter at pH 7.0, leading to a reduced diameter increase due to acidosis (P < 0.05, not indicated). NO donor (SIN-1 or SNAP) and 8-BrcGMP application during NOS inhibition reestablished resting diameter at pH 7.4 and completely restored vasodilator response to pH 7.0. The NO-independent vasodilators papaverine or nimodipine only reestablished vessel diameter at pH 7.4, the MCA response to extraluminal acidosis remained significantly reduced. #P < 0.05, significant differences in vessel diameters at pH 7.4; *P < 0.05, significant differences in vessel diameters at pH 7.0.

Soluble Guanylyl Cyclase Inhibition and Subsequent cGMP Application

Soluble guanylyl cyclase inhibition with ODQ reduced vessel diameters at physiological extraluminal buffer solutions to ~80% and reduced acidotic vasodilation to a nonsignificant diameter increase of 3.8 ± 4.8 µm (from 109.2 ± 7.9 µm at pH 7.4 to 113.0 ± 6.6 µm at 7.0) (P > 0.10). Vasodilatory reaction to acidosis was, therefore, abolished during soluble guanylyl cyclase inhibition. While reestablishing vessel diameter at pH 7.4 by the addition of the cell-permeable cGMP analog 8-BrcGMP extraluminally during continued guanylyl cyclase inhibition, the vessel reactivity to acidosis was completely restored (Fig. 3).


View larger version (30K):
[in this window]
[in a new window]
  		Fig. 3.   Effect of extraluminal application of 8-BrcGMP (30 µM, study VI) on soluble guanylyl cyclase inhibition by ODQ (5 µM) on resting MCA diameter at pH 7.4 and vasodilator response to extraluminal acidosis. Soluble guanylyl cyclase inhibition by ODQ reduced resting diameter at pH 7.4 (P > 0.05) and abolished diameter increase at pH 7.0. 8-BrcGMP application during soluble guanylyl cyclase inhibition reestablished resting diameter at pH 7.4 and completely restored vasodilator response to pH 7.0. *P < 0.05, significant differences in vessel diameters at pH 7.0.

Source of Basal NO (nNOS Inhibition, Denervation of Perivascular Nerves, or Removal of Endothelium)

NOS inhibition with 7-NI reduced vessel diameters at physiological extraluminal buffer solutions slightly and not significantly to ~89%, and acidotic vasodilation was significantly attenuated to a diameter increase of only 13.7 ± 13.3 µm (from 126.5 ± 10.6 µm at pH 7.4 to 140.2 ± 11.3 µm at 7.0) (P < 0.05). Thus vasodilation to acidotic extraluminal buffer was significantly reduced to ~11% during NOS inhibition with 7-NI. Extraluminal addition of the cell-permeable cGMP analog 8-BrcGMP during further NOS inhibition with 7-NI restored vessel diameter at pH 7.4 and reestablished the pH-dependent vessel reactivity (50.6 ± 20.5 µm diameter increase due to reduced extraluminal pH) (Fig. 4).


View larger version (30K):
[in this window]
[in a new window]
  		Fig. 4.   Effect of extraluminal application of 8-BrcGMP (30 µM, study VII) on NOS inhibition by 7-NI (100 µM)-induced attenuation of resting MCA diameter at pH 7.4 and vasodilator response to extraluminal acidosis. NOS inhibition by 7-NI reduced resting diameter at pH 7.4 (P > 0.05) as well as diameter increase at pH 7.0 (P < 0.05, not indicated). 8-BrcGMP application during NOS inhibition by 7-NI reestablished resting diameter at pH 7.4 and completely restored vasodilator response to pH 7.0. #P < 0.05, significant differences in vessel diameters at pH 7.4; *P < 0.05, significant differences in vessel diameters at pH 7.0.

To test the specificity of 7-NI for nNOS in our in vitro system of the isolated MCA, the vasodilation to the endothelium-dependent vasodilator acetylcholine was compared before and after 7-NI application. The acetylcholine-induced dilation of 53 ± 14 µm at baseline conditions was reduced to 20 ± 15 µm during NOS inhibition with 7-NI, suggesting an inhibitory effect of 7-NI on NOS located in endothelial cells.

To further clarify the isoform involved and the origin of NO responsible for vascular pH reactivity, nonpharmacological approaches were chosen. In one set of experiments, the NOS-containing perivascular neuronal source was removed, and in another set of experiments the endothelium was removed.

In study VIII, 2 wk after extracranial dissection of the NCN and PSF from the sphenopalatine ganglion, the vasodilation response to acidotic MOPS solution was nearly abolished compared with the sham-operated control group (P < 0.01), and extraluminal application of cGMP analog in the dissected study group significantly increased the vasodilation response to extraluminal acidosis (Fig. 5A). Vasodilation reactivity to potassium (extraluminal, 20 mM, NO-independent vasodilator) or acetylcholine (intraluminal, endothelium-dependent vasodilator) did not differ between the dissected study group and the sham-operated study group (Fig. 5B), suggesting a specific role of nNOS from perivascular nerves during pH reactivity.


View larger version (28K):
[in this window]
[in a new window]
  		Fig. 5.   Effect of chronic denervation of neuronal NOS (nNOS)-containing perivascular nerve fibers in the adventitial layer of the MCA, originating from the NCN and PSF from the sphenopalatine ganglion due to extracranial transsection of these nerve bundles at the ethmoidal foramen 14 days before experiments. A: resting MCA diameter at pH 7.4 was not changed in denervated arteries (PSF/NCN cut) compared with arteries from sham-operated animals. In contrast, vasodilator response to extraluminal acidosis was nearly abolished after PSF/NCN dissection. Extraluminal application of cGMP (30 µM) significantly increased vasodilator response to extraluminal acidosis in the denervated group, reaching dilations comparable to the sham-operated group. *P < 0.05, significant difference in vessel diameters at pH 7.0. B: endothelium-dependent (intraluminal application of 100 µM acetylcholine) as well as NO-independent (extraluminal application of 20 mM K+) vasodilations were unchanged in the PSF/NCN dissection group compared with the sham-operated group.

After removal of the endothelium by intraluminal application of the detergent CHAPS in study IX, vessel diameter at pH 7.4 increased by 26% (P < 0.05). The effectiveness of endothelium removal was tested by acetylcholine application, leading to a 40-50% diameter increase during physiological conditions, which was completely abolished by CHAPS as well as air application in studies IX and X. During extraluminal application of acidotic buffer solution in endothelium-denuded vessels in study IX, the diameters still significantly increased by 28.5 ± 6.9 µm (P < 0.05) compared with the elevated baseline. The vasodilator response to pH 7.0 after CHAPS was therefore reduced due to baseline diameter increase (Fig. 6). In study X, in which the vascular endothelium was carefully removed by transient air application, resting diameter at pH 7.4 did not change. The response to pH 7.0 was totally preserved and did not differ between the condition with intact endothelium and the condition in which the endothelium was removed (Fig. 6).


View larger version (42K):
[in this window]
[in a new window]
  		Fig. 6.   Effect of removal of the endothelium on resting diameter at pH 7.4 and vasodilator response to extraluminal acidosis. Transient intraluminal application of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) (0.3% in intraluminal buffer solution for 2.5 min, study IX) increased vessel diameter at pH 7.4 #P < 0.05 (A), whereas transient intraluminal application of air (8 ml for 15 min, study X) had no effect on resting diameter at pH 7.4 (B). Vasodilator response to pH 7.0 was preserved (significant diameter increase in endothelium-denuded vessels, P < 0.05, not indicated). Compared with the baseline response, vasodilator response after CHAPS was significantly reduced (P < 0.05, not indicated), whereas the response was completely preserved after transient intraluminal application of air. Acetylcholine (intraluminal application, 100 µM)-induced vasodilation of 40-50% during baseline conditions (endothelium intact, pH 7.4) was totally abolished after removal of endothelium by CHAPS (study IX, A) as well as air (study X, B). *P < 0.05, significant differences in vessel diameters at pH 7.0 or during acetylcholine application compared with resting diameter.

Histochemical Confirmation of Chronic Denervation of NOS-Containing Perivascular Nerves

Under physiological conditions (sham operation), high densities of NOS-containing perivascular nerves were detected in the adventitial layer of the MCA (NADH diaphorase staining). Transsection of NCN and PSF 14 days before the experiments led to a complete loss of these NOS-containing nerve endings. Figure 7 shows the typical finding 2 wk after NCN and PSF transsection compared with nontranssected animals.


View larger version (58K):
[in this window]
[in a new window]
  		Fig. 7.   NADPH diaphorase histochemistry shows a typical example of confirmation of chronic denervation of NOS-containing perivascular nerves. A: under physiological conditions (sham operation), high densities of NOS-containing perivascular nerves were detected in the adventitial layer of the MCA. B: extracranial transsection of the NCN and PSF from the sphenopalatine ganglion at the ethmoidal foramen 14 days before the experiments led to a complete loss of these NADPH diaphorase positive nerve endings.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

There are five key findings of this study. First, the NOS inhibition-induced attenuation of the vasodilator response of the isolated rat MCA to extraluminal acidosis can be fully restored by reestablishing basal NO levels. Second, this permissive role of NO during acidosis-induced vasodilation is to a large degree mediated by a NO-dependent basal cGMP concentration in the vessel. Third, this basal NO-cGMP level in the isolated MCA is mainly produced by NO from the neuronal isoform of the constitutive NOS. Fourth, the endothelium plays no role in pH-dependent diameter changes in the isolated cerebral artery. Finally, the nNOS, providing basal NO, is located in perivascular nerve endings in the adventitial layer of the vessel. NOS-containing nerve endings at the MCA are mostly originating from NCN and PSF.

The model of isolated cerebral arteries allows investigation of mediators or modulators of cerebrovascular reactivity without parenchymal influences. The cannulated and perfused isolated cerebral artery develops spontaneous tone in response to physiological intraluminal pressure without pharmacological intervention (11, 22), and the intact adventitial layer provides a physiological neurotransmitter and neuromodulator tonus from perivascular nerve endings (21). In our model, we used MOPS-buffered physiological salt solution for the extraluminal bath as well as the intraluminal perfusion. Albumin (1%) was added intraluminally to maintain the integrity of the endothelial cells and luminal matrix (11). Changes in pH were induced by the addition of small amounts of HCl to the buffer solution, because MOPS-buffered saline contains no bicarbonate and is, therefore, characterized by CO2-free or at least hypocarbic conditions. Under physiological conditions in the in vivo system, a change in extracellular pH is associated with alterations in CO2 and/or HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>. These molecules may have independent effects on vascular tone. Nevertheless, it has been shown that in the cerebral vasculature a change in CO2 with a maintained extracellular pH has generally little effect on vascular tension in vivo (24) as well as in vitro (38). These findings suggest that the vascular response to CO2 in the brain is mediated by a change in the proton concentration (24, 30). Compared with the reduction of vascular tone reached by using bicarbonate-buffered saline solution, a similar reduction has been shown to occur during lowered extracellular pH using artificial buffer solutions such as HEPES or Tris (3). In the present study, vasoreactivity in the physiological range was tested by changes of extracellular potassium concentrations or pH changes in the extraluminal bath and led to similar diameter changes compared with those shown by others (22, 28).

Relaxation or constriction of cerebrovascular smooth muscle cells occurs due to decreased or increased intracellular Ca2+ concentrations, in part by closing or opening of voltage-gated Ca2+ channels due to hyper- or hypopolarization of the cells. Hypercapnic acidosis, but not normocapnic acidosis, is associated with hyperpolarization (16), suggesting a difference in the mechanisms of action between hypercapnic and normocapnic extracellular acidification. In contrast to experimental models using normocapnic acidification, isolated cerebral arteries studied in bicarbonate-free buffered saline solutions also showed hyperpolarization accompanied by vasodilation during acidification (9), suggesting identical mechanisms of action during physiological hypercapnic acidosis and the HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>/CO2-free, MOPS-buffered in vitro system.

The role of NO in the vascular response to acidosis in the isolated artery has not been investigated in detail so far. In the present study, we have shown that NOS inhibition in the isolated artery led to a significant reduction of resting diameter. Analogous results have been described during NOS inhibition in studies measuring regional cerebral blood flow (rCBF) in the cerebral cortex in vivo (10) or resting basilar artery diameter in vitro (43), indicating a basal NO tonus produced directly by the vascular compartment. In addition, we have shown that vasodilation to extraluminal acidosis was nearly abolished during NOS inhibition. The same results have been shown for the reactivity of the basilar artery to acidosis (43). Intracerebral arterioles (38-55 µm diameter), however, have been shown to dilate to pH 6.8 buffer solution independent of NO (29). These inconsistent findings suggest a difference in the NO dependency of the reactivity of intracerebral arterioles compared with the MCA itself. In addition, it has been shown that hypercapnic cerebral blood flow (CBF) response is not uniformly dependent on NO, and vascular reactivity to high arterial partial pressure of CO2 is completely independent of the bioradical NO (18). Ngai and Winn (29) used buffer solution with lower pH value in their study, and this may, in addition, explain the differences in the NO dependency between the results by Ngai and Winn and our study.

In the present study, we have shown that the acidotic vasodilation was solely dependent on basal NO concentration because NOS inhibition nearly abolished vasodilation response to extraluminal acidotic buffer solution. Reestablishing the vascular tone by adding NO donors in the extraluminal bath completely restored pH-dependent reactivity of the isolated MCA. This NO effect seems to be mediated by cGMP, because cGMP application during NOS inhibition as well as soluble guanylyl cyclase inhibition was equally effective in restoring pH reactivity. The NO-independent vasodilators papaverin or nimodipine had no effect on reduced vasoreactivity with extraluminal acidosis during NOS inhibition, suggesting a specific NO-cGMP-mediated mechanism. In accordance with our findings are data by You et al. (43) demonstrating that high CO2 elicits vasodilation of the isolated basilar artery by a mechanism that depends on basal NO levels but which is independent of increased NO production by elevated NOS activity. In addition, no increase of basal cGMP concentration was observed during CO2-induced vasodilation (43). In a recent study by Wang et al. (41), an only transient increase in cGMP production within the first minute of hypercapnic acidosis has been shown in neuronal cells in vitro. The cGMP concentration then returned to baseline despite ongoing acidosis, suggesting that this transient increase does not account for vascular reaction in intact tissue.

To our knowledge the present study is the first to show the modulatory action of NO during acidotic vasodilation in the rat isolated MCA. The permissive effect of NO-cGMP has been shown for the hypercapnic rCBF response in vivo in the cerebral cortex of rats (20). Kontos and Wei (25), however, did not confirm the permissive role of NO via cGMP in the cat cerebral circulation. The authors suggest that arginine analogs inhibit hypercapnic vasodilation by blocking ATP-sensitive K+ channels independently of cGMP, and that these ATP-sensitive K+ channels may have an arginine site that influences their function in contrast to the proposed modulation by NO, either directly or via cGMP.

There are various potential cellular sources for the basal NO level in the cerebrovascular environment. Inhibition of the neuronal isoform of NOS by 7-NI in vivo led to a significant reduction of hypercapnic rCBF responses in a permissive way (31). Analogous results have been shown in the present study for the reaction of the isolated MCA to extraluminal acidosis. However, the specificity of the NOS inhibitor 7-NI in in vitro as well as in vivo experiments has been discussed controversially (14, 27). We, therefore, tested the effect of 7-NI on the vasodilator response to acetylcholine in our model. Acetylcholine has been shown to induce dilation at least in part via NO production in vascular endothelium (12). The acetylcholine response was reduced by ~50% during 7-NI application, pointing to a partial inhibition of NOS within the vascular endothelium, and a possible role of NOS in endothelial cells during acidotic vasodilation cannot be excluded. Besides morphological findings (35), recently Benyó et al. (4) provided strong functional evidence that the neuronal isoform of NOS is also present in cerebrovascular endothelial cells. They demonstrated a partial inhibition of vasodilation to acetylcholine during 7-NI inhibition in the isolated rat basilar artery, whereas the response to bradykinin, a specific eNOS-dependent vasodilator, was unchanged. Therefore, it can be suggested that nNOS is also involved in the endothelium-dependent vasodilation to acetylcholine, and 7-NI may indeed act as a specific inhibitor of nNOS in the in vitro system. The possibility that acetylcholine stimulates NO release from perivascular nerves can be excluded in their study as well as in our study, because removal of the endothelium completely abolishes relaxation to acetylcholine.

To further identify the cellular source of NO and to exclude the possibility of an involvement of nNOS located in endothelial cells, we performed further experiments independent of pharmacological NOS inhibition. Removal of the endothelium in vivo as well as in vitro had no effect on hypercapnic or acidotic vessel responses (38, 42, 43), suggesting a more specific role for nNOS distinct from endothelial cells in the pH-dependent vasoreactivity. In the present study, removal of the endothelium with transient intraluminal application of the detergent CHAPS increased vessel diameters at physiological pH 7.4 by 26%. Besides a possible vasodilator tonus due to NO production, vascular endothelial cells have the ability to produce a variety of vasoconstrictors, such as thromboxane and endothelins. To test whether the observed increase in vessel diameters after endothelium removal by CHAPS, in our study, suggests a stronger influence of constricting factors rather than vasodilating factors on vascular smooth muscle cells or whether the vessels were simply damaged by the denudation procedure, we very carefully added air intravascularly for a short time period in another group of arteries. This procedure did not change resting diameter at physiological pH. Both procedures totally abolished the endothelium-dependent vasodilation to acetylcholine. After removal of the endothelium by application of air, the vessel response to acidic extraluminal environment was unchanged compared with the reaction under physiological conditions. Because the observed increase in resting diameter after removal of the endothelium with CHAPS has not been shown by others and is not consistent with our study group using air, the diameter increase after CHAPS application may indeed be caused by a functional impairment of the smooth muscle cell layer itself, and the reactivity to acidosis cannot be properly interpreted in this study group. However, because of our findings using air to carefully remove the endothelium without diameter increases after the procedure, an endothelial component in the NO-dependent response to acidosis can be excluded.

Because 7-NI has been shown to partially inhibit NOS from vascular endothelium in our in vitro system in addition to its specific effect on extra-endothelial nNOS, we studied the specific role of NOS-positive perivascular nerves (17), the main compartment containing nNOS in the isolated artery. The presence of NOS-positive nerves surrounding the MCA in the sham-operated group, and the efficacy of denervation was examined in whole mount preparations processed for NADPH-diaphorase histochemistry, a procedure that stains NOS-containing neurons (17). Transsection of the NCN and the PSF originating from the spenopalatine ganglion at the ethmoidal foramen led to a complete loss of NOS-positive neuronal endings surrounding the MCA. This result demonstrates the main source of nNOS in the NCN and the PSF originating from the spenopalatine ganglion. In these denervated arteries, reactivity to pH changes was nearly abolished compared with arteries harvested from sham-operated animals, whereas the NO-independent vasodilation to 20 mM potassium as well as the endothelium-dependent vasodilation to acetylcholine was unchanged. cGMP application in denervated arteries reestablished the reactivity to acidosis. These results further support our hypothesis that the basal NO concentration in the MCA is indeed provided by NOS-containing perivascular nerves originating from the spenopalatine ganglion at the ethmoidal foramen. It has been shown that in vivo the rCBF response to hypercapnia was only slightly and not significantly reduced from 2.23 ± 0.3%/mmHg to 1.78 ± 0.5%/mmHg, when NCN and PSF were chronically transsected (33) or no influence at all was observed (19). Both large arteries and microvessels contribute to reductions in vascular resistance during hypercapnia in vivo. The different vascular compartments investigated may explain the discrepancy between these in vivo studies in which microcirculatory changes in MCA territory of the parietal cortex were examined and the present in vitro study of the MCA itself. Because NCN and PSF fibers only project to large cerebral arteries in the anterior and MCA territory, the lack of influence of NCN and PSF denervation in microcirculation studies is not unexpected. The denervation-induced changes in large cerebral arteries may be compensated by the downstream smaller vessels with a net result of no alteration in blood flow. In addition, basal NO in the intact cerebral tissue is not only provided by perivascular nerve endings originating from the sphenopalatine ganglion and the trigeminal system. nNOS is constitutively expressed in neurons and astrocytes. nNOS-positive interneurons have been shown in a small percent in deeper lamina of the somatosensory cortex (8) and to a larger extent, surrounding larger cerebral arteries as well as cerebral microvessels (17).

The functional significance of the present study is, therefore, on the one hand, the role of basally released NO from perivascular nerves in the reactivity of large cerebral arteries to extraluminal acidosis in the context of systemic hypercapnia. Within the blood flow response to hypercapnia in vivo, NOS-containing nerve fibers from extracerebral origin provide only part of the basal NO level in the parenchymal tissue, as shown by the unchanged microcirculatory blood flow response after NCN and PSF dissection. On the other hand, however, the present study provides for the first time evidence that the reactivity of large cerebral arteries can be significantly impaired by a reduction of the basal NO level in the direct vicinity of the arteries itself, provided by perivascular nerve fibers. This impairment may play a significant role during pathophysiological conditions as focal ischemia or brain trauma, conditions that are accompanied by tissue acidosis independent of systemic hypercapnia.

The molecular mechanism of acidosis-induced vasodilation and the role of the basal NO level in the vicinity of cerebral arteries, providing physiological smooth muscle cell function and pH-dependent vascular reactivity, are not clear so far. Hypercapnic and normocapnic acidosis (37) as well as acidosis in HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>/CO2-free systems (3) are associated with intracellular acidosis in vascular tissues. Nevertheless, there is evidence that changes in intracellular pH are not responsible for smooth muscle cell relaxation during extracellular pH reduction (1). Thus the question arises how the information of increased extracellular proton concentration is transduced into vasodilation. Hyper- or hypopolarizing K+ channel activity has been shown to be modulated by NO or cGMP, and by the extracellular proton concentration (5, 6, 25, 34). Recent findings provide strong evidence for a contribution of ATP-sensitive potassium channels in the vasodilation of cerebral arteries to acidosis (15, 23, 25). The results concerning a role for K+ channels in hypercapnia-induced cerebrovasodilation, however, are inconclusive, because others did not find evidence for a role of K+ channels in hypercapnia-induced cerebrovasodilation under physiological conditions (32, 40). Further experiments have to be performed to solve this discrepancy.

In conclusion, our findings have shown that acidotic vasodilation in the isolated cerebral artery depends on a basal NO concentration in a modulatory sense. This basal NO level is provided by constitutive nNOS located in perivascular nerves. The endothelium plays no role in the vasodilation to extracellular acidosis.


    ACKNOWLEDGEMENTS

The authors thank Marco Foddis for technical assistance.


    FOOTNOTES

* U. Lindauer and A. Kunz contributed equally to this work.

This study was supported by the Deutsche Forschungsgemeinschaft (SFB 507/A6 and Di 454/8-2), by the Hermann and Lilly Schilling Stiftung, by the Humboldt University, Berlin (HSP III) and by the German-Israeli Foundation for Scientific Research and Development (I 0520-233.01/96).

Address for reprint requests and other correspondence: U. Lindauer, Dept. of Experimental Neurology, Humboldt Univ., Charité Hospital, 10098 Berlin, Germany (E-mail: ute.lindauer{at}charite.de).

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 2 August 1999; accepted in final form 9 May 2001.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

1.   Aalkjaer, C, and Poston L. Effects of pH on vascular tension: which are the important mechanisms? J Vasc Res 33: 347-359, 1996[Web of Science][Medline].

2.   Alexander, MY, Brosnan MJ, Hamilton CA, Fennell JP, Beattie EC, Jardine E, Heistad DD, and Dominiczak AF. Gene transfer of endothelial nitric oxide synthase but not Cu/Zn superoxide dismutase restores nitric oxide availability in the SHRSP. Cardiovasc Res 47: 609-617, 2000[Abstract/Free Full Text].

3.   Austin, C, and Wray S. Extracellular pH signals affect rat vascular tone by rapid transduction into intracellular pH changes. J Physiol (Lond) 466: 1-8, 1993[Abstract/Free Full Text].

4.   Benyó, Z, Lacza Z, Hortobágyi T, Görlach C, and Wahl M. Functional importance of neuronal nitric oxide synthase in the endothelium of rat basilar arteries. Brain Res 877: 79-84, 2000[Web of Science][Medline].

5.   Bolotina, VM, Najibi S, Palacino JJ, Pagano PJ, and Cohen RA. Nitric oxide directly activates calcium-dependent potassium channels in vascular smooth muscle. Nature 368: 850-853, 1994[Medline].

6.   Bonnet, P, Rusch NJ, and Harder DR. Characterization of an outward K+ current in freshly dispersed cerebral arterial muscle cells. Pflügers Arch 418: 292-296, 1991[Web of Science][Medline].

7.   Branston, NM. The physiology of the cerebrovascular parasympathetic innervation. Br J Neurosurg 9: 319-329, 1995[Web of Science][Medline].

8.   Caggiano, AO, and Kraig RP. Neuronal nitric oxide synthase expression is induced in neocortical astrocytes after spreading depression. J Cereb Blood Flow Metab 18: 75-87, 1998[Web of Science][Medline].

9.   Dietrich, HH, and Dacey RGJ Effects of extravascular acidification and extravascular alkalinization on constriction and depolarization in rat cerebral arterioles in vitro. J Neurosurg 81: 437-442, 1994[Web of Science][Medline].

10.   Dirnagl, U, Lindauer U, and Villringer A. Role of nitric oxide in the coupling of cerebral blood flow to neuronal activation in rats. Neurosci Lett 149: 43-46, 1993[Web of Science][Medline].

11.   Duling, BR, and Rivers RJ. Isolation, cannulation, and perfusion of microvessels. In: Microcirculatory Technology, edited by Baker CH, and Nastuk WL.. Orlando, FL: Academic, 1986, p. 265-280.

12.   Elhusseiny, A, and Hamel E. Muscarinic-but not nicotinic-acetylcholine receptors mediate a nitric oxide-dependent dilation in brain cortical arterioles: a possible role for the M5 receptor subtype. J Cereb Blood Flow Metab 20: 298-305, 2000[Web of Science][Medline].

13.   Endres, M, Laufs U, Huang Z, Nakamura T, Huang P, Moskowitz MA, and Liao JK. Stroke protection by 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibitors mediated by endothelial nitric oxide synthase. Proc Natl Acad Sci USA 95: 8880-8885, 1998[Abstract/Free Full Text].

14.   Fabricius, M, Rubin I, Bundgaard M, and Lauritzen M. NOS activity in brain and endothelium: relation to hypercapnic rise of cerebral blood flow in rats. Am J Physiol Heart Circ Physiol 271: H2035-H2044, 1996[Abstract/Free Full Text].

15.   Faraci, FM, Breese KR, and Heistad DD. Cerebral vasodilation during hypercapnia. Role of glibenclamide-sensitive potassium channels and nitric oxide. Stroke 25: 1679-1683, 1994[Abstract].

16.   Harder, DR. Effect of H+ and elevated PCO2 on membrane electrical properties of rat cerebral arteries. Pflügers Arch 394: 182-185, 1982[Web of Science][Medline].

17.   Iadecola, C, Beitz AJ, Renno W, Xu X, Mayer B, and Zhang F. Nitric oxide synthase-containing neural processes on large cerebral arteries and cerebral microvessels. Brain Res 606: 148-155, 1993[Web of Science][Medline].

18.   Iadecola, C, and Zhang F. Nitric oxide-dependent and -independent components of cerebrovasodilation elicited by hypercapnia. Am J Physiol Regulatory Integrative Comp Physiol 266: R546-R552, 1994[Abstract/Free Full Text].

19.   Iadecola, C, Zhang F, and Xu X. Role of nitric oxide synthase-containing vascular nerves in cerebrovasodilation elicited from cerebellum. Am J Physiol Regulatory Integrative Comp Physiol 264: R738-R746, 1993[Abstract/Free Full Text].

20.   Iadecola, C, Zhang F, and Xu X. SIN-1 reverses attenuation of hypercapnic cerebrovasodilation by nitric oxide synthase inhibitors. Am J Physiol Regulatory Integrative Comp Physiol 267: R228-R235, 1994[Abstract/Free Full Text].

21.   Ignacio, CS, Curling PE, Childres WF, and Bryan RMJ Nitric oxide-synthesizing perivascular nerves in the rat middle cerebral artery. Am J Physiol Regulatory Integrative Comp Physiol 273: R661-R668, 1997[Abstract/Free Full Text].

22.   Johnson, TD, Marrelli SP, Steenberg ML, Childres WF, and Bryan RM. Inward rectifier potassium channels in the rat middle cerebral artery. Am J Physiol Regulatory Integrative Comp Physiol 274: R541-R547, 1998[Abstract/Free Full Text].

23.   Kinoshita, H, and Katusic ZS. Role of potassium channels in relaxations of isolated canine basilar arteries to acidosis. Stroke 28: 433-437, 1997[Abstract/Free Full Text].

24.   Kontos, HA, Raper AJ, and Patterson JL. Analysis of vasoactivity of local pH, PCO2 and bicarbonate on pial vessels. Stroke 8: 358-360, 1977[Abstract/Free Full Text].

25.   Kontos, HA, and Wei EP. Arginine analogues inhibit responses mediated by ATP-sensitive K+ channels. Am J Physiol Heart Circ Physiol 271: H1498-H1506, 1996[Abstract/Free Full Text].

26.   Lindauer, U, Megow D, Matsuda H, and Dirnagl U. Nitric oxide: a modulator, but not a mediator, of neurovascular coupling in rat somatosensory cortex. Am J Physiol Heart Circ Physiol 277: H799-H811, 1999[Abstract/Free Full Text].

27.   Moore, PK, Wallace P, Gaffen Z, Hart SL, and Babbedge RC. Characterization of the novel nitric oxide synthase inhibitor 7-nitro indazole and related indazoles: antinociceptive and cardiovascular effects. Br J Pharmacol 110: 219-224, 1993[Web of Science][Medline].

28.   Ngai, AC, and Winn HR. Effects of adenosine and its analogues on isolated intracerebral arterioles. Extraluminal and intraluminal application. Circ Res 73: 448-457, 1993[Abstract/Free Full Text].

29.   Ngai, AC, and Winn HR. Modulation of cerebral arteriolar diameter by intraluminal flow and pressure. Circ Res 77: 832-840, 1995[Abstract/Free Full Text].

30.   Niwa, K, Lindauer U, Villringer A, and Dirnagl U. Blockade of nitric oxide synthesis in rats strongly attenuates the CBF response to extracellular acidosis. J Cereb Blood Flow Metab 13: 535-539, 1993[Web of Science][Medline].

31.   Okamoto, H, Hudetz AG, Roman RJ, Bosnjak ZJ, and Kampine JP. Neuronal NOS-derived NO plays permissive role in cerebral blood flow response to hypercapnia. Am J Physiol Heart Circ Physiol 272: H559-H566, 1997[Abstract/Free Full Text].

32.   Reid, JM, Paterson DJ, Ashcroft FM, and Bergel DH. The effect of tolbutamide on cerebral blood flow during hypoxia and hypercapnia in the anaesthetized rat. Pflügers Arch 425: 362-364, 1993[Web of Science][Medline].

33.   Reuter, U, Weber JR, Gold L, Arnold G, Wolf T, Dreier J, Lindauer U, and Dirnagl U. Perivascular nerves contribute to cortical spreading depression-associated hyperemia in rats. Am J Physiol Heart Circ Physiol 274: H1979-H1987, 1998[Abstract/Free Full Text].

34.   Robertson, BE, Schubert R, Hescheler J, and Nelson MT. cGMP-dependent protein kinase activates Ca-activated K channels in cerebral artery smooth muscle cells. Am J Physiol Cell Physiol 265: C299-C303, 1993[Abstract/Free Full Text].

35.   Stanarius, A, Seidel B, and Wolf G. Neuronal nitric oxide synthase in the vasculature of the rat brain: an immunocytochemical study using the tyramide signal amplification technique. J Neurocytol 27: 731-736, 1998[Web of Science][Medline].

36.   Suzuki, N, Fukuuchi Y, Koto A, Naganuma Y, Isozumi K, Konno S, Gotoh J, and Shimizu T. Distribution and origins of cerebrovascular NADPH-diaphorase-containing nerve fibers in the rat. J Auton Nerv Syst 49: S51-S54, 1994.

37.   Tian, R, Vogel P, Lassen NA, Mulvany MJ, Andreasen F, and Aalkjaer C. Role of extracellular and intracellular acidosis for hypercapnia-induced inhibition of tension of isolated rat cerebral arteries. Circ Res 76: 269-275, 1995[Abstract/Free Full Text].

38.   Toda, N, Hatano Y, and Mori K. Mechanisms underlying response to hypercapnia and bicarbonate of isolated dog cerebral arteries. Am J Physiol Heart Circ Physiol 257: H141-H146, 1989[Abstract/Free Full Text].

39.   Tomimoto, H, Akiguchi I, Wakita H, Nakamura S, and Kimura J. Distribution of NADPH-diaphorase in the cerebral blood vessels of rats: a histochemical study. Neurosci Lett 156: 105-108, 1993[Web of Science][Medline].

40.   Wang, Q, Bryan RMJ, and Pelligrino DA. Calcium-dependent and ATP-sensitive potassium channels and the `permissive' function of cyclic GMP in hypercapnia-induced pial arteriolar relaxation. Brain Res 793: 187-196, 1998[Web of Science][Medline].

41.   Wang, Q, Bryowsky J, Minshall RD, and Pelligrino DA. Possible obligatory functions of cyclic nucleotides in hypercapnia-induced cerebral vasodilation in adult rats. Am J Physiol Heart Circ Physiol 276: H480-H487, 1999[Abstract/Free Full Text].

42.   Wang, Q, Pelligrino DA, Koenig HM, and Albrecht RF. The role of endothelium and nitric oxide in rat pial arteriolar dilatory responses to CO2 in vivo. J Cereb Blood Flow Metab 14: 944-951, 1994[Web of Science][Medline].

43.   You, JP, Wang Q, Zhang W, Jansen-Olesen I, Paulson OB, Lassen NA, and Edvinsson L. Hypercapnic vasodilatation in isolated rat basilar arteries is exerted via low pH and does not involve nitric oxide synthase stimulation or cyclic GMP production. Acta Physiol Scand 152: 391-397, 1994[Web of Science][Medline].

Am J Physiol Heart Circ Physiol 281(3):H1353-H1363
0363-6135/01 $5.00 Copyright © 2001 the American Physiological Society


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. D. Bauser-Heaton, J. Song, and H. G. Bohlen
Cerebral microvascular nNOS responds to lowered oxygen tension through a bumetanide-sensitive cotransporter and sodium-calcium exchanger
Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2166 - H2173.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. D. Bauser-Heaton and H. G. Bohlen
Cerebral microvascular dilation during hypotension and decreased oxygen tension: a role for nNOS
Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2193 - H2201.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
F. M. Faraci
nNOS-Containing Perivascular Nerves: Stranger by the Minute
Circ. Res., July 12, 2002; 91(1): 7 - 8.
[Full Text] [PDF]

Home page
 StrokeHome page
T. Horiuchi, H. H. Dietrich, K. Hongo, T. Goto, and R. G. Dacey Jr
Role of Endothelial Nitric Oxide and Smooth Muscle Potassium Channels in Cerebral Arteriolar Dilation in Response to Acidosis
Stroke, March 1, 2002; 33(3): 844 - 849.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lindauer, U.
Right arrow Articles by Dirnagl, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lindauer, U.
Right arrow Articles by Dirnagl, U.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online