| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Division of Cardiology, Departments of Medicine, Molecular Pharmacology and Medicinal Chemistry and the Feinberg Cardiovascular Research Institute, Northwestern University Medical School, Chicago, Illinois 60611; and 2 Department of Physiology, University of Wisconsin Medical College, Madison, Wisconsin 53706
 |
ABSTRACT |
|---|
 TOP
 ABSTRACT
 INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
|
|---|
We investigated the possibility that the Ca2+ channel agonist FPL-64176 (FPL) might also activate the cardiac sarcoplasmic reticulum (SR) Ca2+ release channel ryanodine receptor (RyR). The effects of FPL were tested on single channel activity of purified and crude vesicular RyR (RyR2) isolated from human and dog hearts using the planar lipid bilayer technique. FPL (100-200 µM) increased single channel open probability (Po) when added to the cytoplasmic side of the channel (Po = 0.070 ± 0.021 in control RyR2; 0.378 ± 0.086 in 150 µM FPL, n = 9, P < 0.01) by prolonging open times and decreasing closed times without changing current magnitude. FPL had no effect on Po when added to the trans (luminal) side of the bilayer (Po = 0.079 ± 0.036 in control and 0.103 ± 0.066 in FPL, n = 4, no significant difference). The bell-shaped [Ca2+] dependence of [3H]ryanodine binding and of Po was altered by FPL, suggesting that the mechanism by which FPL increases channel activity is by an increase in Ca2+-induced activation at low [Ca2+] (without a change in threshold) and suppression of Ca2+-induced inactivation at high [Ca2+]. However, the fact that inactivation was restored at elevated [Ca2+] suggests a competitive interaction between Ca2+ and FPL on inactivation. FPL had no effect on RyR skeletal channels (RyR1), where Po was 0.039 ± 0.005 in control versus 0.030 ± 0.006 in 150 µM FPL (no significant difference). These results suggest that, in addition to its ability to activate the L-type Ca2+ channels, FPL activates cardiac RyR2 primarily by reducing the Ca2+ sensitivity of inactivation.
sarcoplasmic reticulum; heart; calcium release channel; human; dog
| |
INTRODUCTION |
|---|
|
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
|
|---|
THERE ARE SEVERAL CLASSES of pharmacological agents known to activate L-type (voltage-gated) Ca2+ channels: dihydropyridines (DHP) such as BAY K 8644 (BAYK) and the benzoylpyrole derivative FPL-64176 (FPL). The mechanism by which BAYK alters channel gating is thought to involve a change in mode from normal gating to one with a high probability of opening (6). A similar mechanism is likely to account for the actions of FPL, although its precise interactions with the Ca2+ channel are less well understood than for BAYK. For example, it is known that FPL, like BAYK, prolongs action potential duration and causes a positive inotropic effect in guinea pig papillary muscle, both of which are likely to result from increased L-type Ca2+ channel current (ICa,L) magnitude (14). Both agents induce a hyperpolarizing shift in activation and inactivation by ~10 mV (3). However, FPL slows the rates of ICa,L activation and inactivation, whereas BAYK increases current magnitude while accelerating the rate of decay (3, 14). These effects of FPL on contraction and ICa,L are presumably a result of its ability to prolong single channel open time during depolarization and immediately after repolarization, with little effect on closed times and latency to first openings (3).
Because of the differences in drug actions on ICa,L, there has been additional exploration of whether or not these agonists also share binding sites in the Ca2+ channel itself. FPL did not affect [3H]PN 200-110 binding; thus DHPs and FPL are thought to bind to different sites on the channel protein (16, 23). However, electrophysiological evidence showed that single-channel open probability (Po) for a BAYK-FPL mixture exhibited a lower Po than either alone (11). This result was interpreted to suggest that although these agents bind to different sites on the Ca2+ channel, a functional or physical interaction does in fact exist between them.
Another interesting difference between the effects of the two ICa,L agonists is that BAYK directly increases frequency of Ca2+ sparks whereas FPL does not (10). These results were interpreted to suggest that one of the effects of BAYK binding to the Ca2+ channel may be to alter Ca2+ release from the sarcoplasmic reticulum (SR). This action might occur via a connection between the sarcolemmal Ca2+ channel and the release channel in the SR membrane, an effect not shared by FPL (10).
We considered the possibility that, in addition to their effects on ICa,L, these agents might also have direct effects on cardiac ryanodine receptors (RyR2) that could influence their ability to regulate contraction. We (18) recently reported that, in addition to an effect on ICa,L, BAYK and its analogs also activate the purified cardiac SR calcium release channel incorporated in artificial lipid bilayers. Interestingly, the mechanism by which the channel is activated may involve a suppression of Ca2+-induced inactivation of RyR2 that occurs at high concentrations of Ca2+. However, this effect was only observed in purified channels and was not observed using reconstituted channels from crude microsomal vesicles. This was the first report of a direct action of BAYK on any other Ca2+ channel aside from that located in the surface membrane of many cell types and might contribute to the cellular actions of DHP agonists.
The purpose of this study was to extend these observations to the second class of Ca2+ channel agonists represented by FPL. In particular, we wanted to determine whether FPL also activates RyR2. If so, does it share a common mechanism of action with the DHP agonists? One implication of a shared mechanism would be the existence of a common or overlapping DHP binding site on the RyR2 that acts as a putative calcium channel agonist receptor. Finally, we compared the effects of FPL on dog and human RyR2 to determine whether or not channel modification occurs in human channels in addition to animal models.
| |
METHODS |
|---|
|
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
|
|---|
Single channel studies. Dog hearts were obtained from animals anesthetized with pentobarbital sodium (35 mg/kg iv) before removal of the heart. Cat heart and skeletal (gastrocnemius) preparations were obtained in the same manner. Human hearts were obtained from three normal patients who died from noncardiac disease whose hearts were donated for research purposes. Protocols for animal and human tissue use were reviewed and approved by the Institutional Animal Care and Use Committee and the Internal Review Board at Northwestern University, respectively.
Crude microsomes were obtained from the skeletal muscle and left ventricle with differential centrifugation. Purification of Ca2+ release channels was performed using 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate solubilization of heavy SR vesicles with subsequent reconstitution of purified protein into proteoliposomes (21). Recordings of activity of purified channels were obtained using the planar lipid bilayer technique with 250 mmol/l KCl and 10 mmol/l HEPES (pH 7.2) on the cis (cytoplasmic) and trans (luminal) sides of the bilayer. The trans side also contained 1 mmol/l Ca2+. [Ca2+] in the cis chamber was measured by a Ca2+-sensitive electrode to be 5 µM and was unchanged in most experiments unless stated otherwise. In some experiments, admixtures of CaCl2 and EGTA were used to set [Ca2+] in the cis compartment according to Calcium software. Bilayer composition was 0.4 mg each of phosphatidylserine and phosphatidylethanolamine (Avanti Polar Lipids) suspended in 20 µl of n-decane. Channel incorporation took place in the presence of a 250:20 mM KCl concentration gradient. Once a stable bilayer was formed, 3 µl of microsomes or liposomes were added to the cis side of the bilayer during constant stirring. Channel activity indicated successful fusion of a vesicle with the bilayer. In most experiments, the ionic gradient was then abolished by addition of the appropriate amount of 3 M KCl to the trans side of the bilayer. Recordings (usually 2 or 3 in each condition of 30-s duration each) were initiated after 2 to 3 min of stable activity. Pharmacological agents were added directly to either compartment of the bilayer apparatus after control recordings were obtained and the experimental protocol was repeated after 1 min of stirring. Single channel activity was also recorded from channels in heavy SR vesicles. Procedures were identical to those described above for purified channels with the exception of Cs+-methanesulfonate substitution for KCl to avoid interference from K+ and Cl
channels native
to crude SR vesicles. Dog cardiac SR channels were recorded in the
absence of a Cs+ gradient at different holding potentials.
Dog skeletal SR channels were recorded in the maintained presence of a
Cs+ gradient at 0 mV.
Single channel data were recorded (Axopatch 200 and 200A amplifiers,
Axon Instruments) using pCLAMP version 6 software in data files 30 s in duration at constant holding potentials
(Vh) of ±40 mV in most experiments. Data were
filtered with an eight-pole Bessel filter (model 902, Frequency
Devices) at 2 kHz, digitized at 5 kHz, and analyzed off-line using
half-amplitude threshold algorithms. All chemicals were obtained from
Sigma, with the exception of FPL, which was obtained from BioMol.
[3H]ryanodine binding studies.
Aliquots (80 µg) of microsomal protein were added to an incubation
medium containing 7 nM [3H]ryanodine, 0.2 M KCl, 20 mM
3-(N-morpholino)propanesulfonic acid (pH 7.2), 1 mM EGTA,
and different amounts of CaCl2 to set free
[Ca2+] in the range of 0.01- 10,000 µM. Experimental
modulators were added to reaction mixtures from 100× stocks. The
incubation took place in a volume of 0.1 ml containing ~0.3 pM of the
receptor at 37°C for 90 min. After incubation, bound and free
[3H]ryanodine were separated by rapid filtration onto
Whatman GF/B or GF/C glass fiber filters and the filters were washed
three times with cold distilled water with a Millipore manifold. The filters were placed in liquid scintillation cocktail and counted in a
Beckman LS6500 
-counter. Nonspecific [3H]ryanodine
binding was determined in the presence of 5 µM unlabeled ryanodine
and has been subtracted from all reported values.
Data analysis. Data are presented as means ± SE. Only data obtained from bilayers containing a single channel were included in this study, so Po refers to the activity of a single channel. Data were compared using paired or unpaired Student's t-tests or a one-way analysis of variance (with secondary comparisons made using a Student-Newman-Keuls test). Differences between sample means were considered significant if P < 0.05, unless indicated otherwise, and NS refers to comparisons in which differences did not achieve statistical significance.
| |
RESULTS |
|---|
|
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
|
|---|
Effects of FPL on purified human RyR2.
The effect of FPL on single channel activity using purified RyR2
channels from the human ventricle is shown in Fig.
1A. When FPL (100 µM) was
added to the cis (cytoplasmic) side of the bilayer, channel
open times increased, as did Po. Subsequent
increases in drug concentration caused further increases in
Po and long open times with the result being a
nearly continuously open channel at 200 µM
(Po = 0.913).
|
|
Effects of FPL on channel actvity in dog SR vesicles.
In addition to an effect on purified RyR2, we also investigated the
effects of FPL on single channels from crude SR vesicles. The effects
of FPL on RyR2 single-channel activity in dog SR vesicles are shown in
Fig. 3A. Under control
conditions, channel openings were brief and relatively infrequent,
giving an overall Po of 0.08. After addition of
FPL to the cis side of the bilayer, channel activity was
increased (Po = 0.58) as a result of
prolonged open times. The results obtained under these conditions are
summarized in Fig. 3B and show that FPL activated cardiac
RyR2 channels whether or not they are in the purified or vesicular
form, an observation that is in distinct contrast to that of BAYK
effects on RyR2 (18).
|
Effects of FPL on open and closed characteristics.
To understand the basis for the activating effects of FPL on RyR2, we
analyzed the changes in gating characteristics of dog SR channels
induced by FPL. The histograms shown in Fig.
4 summarize the open duration times (Fig.
4, A and B) and the closed duration times (Fig.
4, C and D) before and during exposure to FPL in
one experiment. Under control conditions, open time durations are best
described as the sum of two exponentials, with the shorter openings
accounting for 41% of total openings. During exposure to FPL, both
short (
O1) and long (O2) open time
durations are increased but there is an especially large increase in
O2. In addition, there is a shift in the proportion of
the two open states such that FPL causes a decrease in the percentage
of O1 (to 20% of total) with a concomitant increase in
the percentage of O2 compared with control recordings.
|
O1 and
O2 durations (Fig. 5A, top) as
well as the proportion of O2 (Fig. 5A,
bottom). Both long and short closed time durations were
significantly decreased (Fig. 5B, top) and the
contribution of long closings to total closed time was diminished (Fig.
5B, bottom).
|
Dependence of FPL activation of RyR2 by cis
[Ca2+].
One of the most common mechanisms by which different agents affect
single RyR2 channel activity is by altering the sensitivity of the
channel to [Ca2+] on the cis side of the
bilayer. We therefore tested this possibility regarding the actions of
FPL on RyR2. Figure 6A shows
the effect of FPL on [3H]ryanodine binding to dog cardiac
SR vesicles at different cis [Ca2+]. In the
absence of FPL, [3H]ryanodine binding is low at 0.1 µM
cis [Ca2+], increases to a maximum at
10-100 µM, and then declines at 1-10 mM. This biphasic
reliance of [3H]ryanodine binding on [Ca2+]
is thought to represent channel activation by calcium over the range of
0.1-100 µM with subsequent development of inactivation occurring
>100 µM. In the presence of FPL, [3H]ryanodine binding
is unchanged at 0.1 µM [Ca2+] but there is an increase
in [3H]ryanodine binding at all subsequent
[Ca2+], which is significant at 
10 µM. These results
suggest that FPL might increase channel activaty at low concentrations
of Ca2+ as well as reduce channel sensitivity to
Ca2+-induced inactivation at higher concentrations.
However, at a [Ca2+] of 10 mM, the inactivation again
becomes manifest, as though high concentrations of Ca2+
were able to successfully compete with the effects of FPL, thus restoring Ca2+-dependent inactivation.
|
Lack of effect of FPL on skeletal channels. To determine whether or not the activation of cardiac RyRs by FPL is unique to that channel isoform, we also investigated the effects of FPL on single channel activity in crude dog skeletal RyR channels (RyR1). In a total of four channels, there was little change in Po (0.039 ± 0.005 in control vs. 0.030 ± 0.006 in 150 µM FPL, NS). Similarly, we found that mean channel open time was 1.07 ± 0.08 ms in control and 1.11 ± 0.14 ms during exposure to FPL (NS).
These results demonstrate that RyR2 is sensitive to activation by FPL, whereas RyR1 is not. In addition, they demonstrate that channel activation by FPL is not a nonspecific interaction with the channel protein, because it only occurs with RyR2 and not with RyR1 under identical recording conditions.| |
DISCUSSION |
|---|
|
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
|
|---|
Mechanisms of RyR2 channel activation by FPL.
Our previous study showed that DHP activation of the RyR2 channel
occurred primarily in the range of [Ca2+] responsible for
RyR inactivation (>100 µM). We proposed that the agonist action was
primarily the result of an effect to suppress Ca2+-induced
inactivation and that this was responsible for channel activation. In
the present study, we found that FPL stimulated both
[3H]ryanodine binding and single channel activity at
[Ca2+] 1 µM. One possible explanation is that the
primary effect of FPL is to reduce the sensitivity of inactivation to
Ca2+. Because there is likely to be extensive overlap in
Ca2+-dependent activation and inactivation, a greater
effect on inactivation might be obvious with little apparent effect on
activation. This might explain why the threshold for activation is not
shifted to lower [Ca2+] with the net result being that of
enhanced channel activity in the activating portion of the curve. It is
also possible but less likely that FPL acts on both
Ca2+-induced activation and inactivation to increase single
channel activity. These effects would explain the apparent
Ca2+-sensitizing effect on activation in the
[Ca2+] range of 1-100 µM and the suppression of
inactivation occurring in the range of 1-10 mM but would not,
however, explain why there is no increase in sensitivity at pCa 7. It
is interesting that the activating effect results from an increase in
the slope of the [Ca2+]-activity relationship rather than
a leftward shift as might be observed with caffeine (17).
This result suggests that there may be an increase in cooperativity
between calcium ions instead of a true sensitization of channel
activity to low [Ca2+]. However, the best
explanation for the effects of FPL to increase RyR2 channel activity is
likely to reside in a suppression of inactivation, possibly by reducing
its sensitivity to Ca2+.
Is the effect of FPL the result of specific interaction with RyR2? The fact that the FPL concentrations required to activate RyR2 are ~500-fold higher than for ICa,L (1, 14) raises the possibility that the effects of FPL on RyR2 are nonspecific. Several observations argue against this point. First, channel activation occurs only when FPL is added to the cis side of the bilayer, suggesting not only a specific interaction with the protein but also binding to a site on the cytoplasmic face of the channel. Second, the inability of FPL to activate RyR1 implies that the interaction with the cardiac isoform is a specific one. Third, the fact that stimulation of both single channel activity and [3H]ryanodine binding by FPL is dependent on cytoplasmic [Ca2+] supports the notion that the drug effect is a result of a specific interaction between drug and channel.
It is also not clear why such high drug concentrations are required, given the higher sensitivity of the ICa,L to FPL. It is important to consider, however, that drug concentration in the bulk solution (plasma or in vitro superfusate) may be very different from intracellular accumulation. For example, the ratio of total tissue concentration to that in the medium for the lipid soluble calcium channel antagonist bepridil varied between 20- and ~50-fold, depending on the cardiac preparation (12). Thus cardiac tissues are capable of concentrating lipophilic agents and it is possible that high concentrations of FPL might exist in bilayers because this agent is very lipophilic. The low water solubility might lead to an accumulation of FPL in SR membranes, thus providing higher local concentrations of drug in the vicinity of RyR2 than is present in bulk aqueous solution. It is also extremely difficult to relate physiological effects to underlying pharmacological interactions between ligand and receptor. The literature is full of disparities between concentrations of drug required to produce a physiological response and those measured to characterize the molecular interaction between a drug and its receptor. For example, it is not clear why cardiac glycosides only affect cardiac cell function in intact tissues and ventricular myocytes at concentrations in the micromolar range (8, 9, and S. Ruch et al., unpublished observations), but the cardiac glycosides are known to inhibit the sarcolemmal Na+-K+-ATPase with dissociation constant values in the range of 2-7 nM (2). The disparity between results from biochemical binding assay and from physiological measurements has still not be explained satisfactorily, even after decades of study. However, it is still assumed that Na+ pump inhibition is the basis for all of the inotropic and toxic actions of cardiac glycosides. These results serve to indicate that measured sensitivity of pharmacological or biochemical interactions between drug and receptor cannot always be translated to sensitivity of physiological response to that drug. Further study is required to resolve this issue.Effects of different classes of Ca2+
channel agonists.
In our previous study of purified RyR2 activation by DHP agonists
(18), we found very similar effects to those reported here
for an entirely different class of agonist represented by FPL. We
observed that BAYK activated the channel with an EC50 of
~3 µM, a much higher efficacy than FPL. However, nearly 100 µM of
another agonist, (+)-SZ-202791, was necessary to produce activation, a
concentration quite similar to FPL. The actions of these DHP agonists
were not influenced by either previous or subsequent addition of equal
or higher concentrations of antagonist [i.e., nifedipine and
(
)-SZ-202791]. These results suggested that if a binding site does
exist on the cytoplasmic face of the RyR2, then it is specific for DHP
agonists because antagonists could neither displace agonists nor
prevent their access to the receptor with prior exposure. In addition,
the Ca2+ dependence of single channel
Po revealed that both agents suppress inactivation to a greater extent than enhancing activation. These observations in turn suggested the presence of a specific agonist receptor on the RyR2. One of the primary goals of the current study was
to determine whether or not channel activation extended to another
known class of ICa,L agonists represented by
FPL. Our current results demonstrate striking similarities in the
actions of BAYK and FPL.
-subunit produced
similar shifts in binding for (+)-PN 200-110 and ()-BAYK is
taken as a strong indication that both agonist and antagonist effects
rely on drug binding to a common receptor (7, 13). In
contrast, other studies (5, 7, 19, 20, 22) provide strong
evidence suggesting distinct binding sites for agonists and
antagonists. Thus it is not clear whether or not DHP agonists share a
common receptor with antagonists on ICa,L.
Given the possibility that there may in fact be a unique receptor for
activation of ICa,L, it is then possible that
other agonists besides DHPs might occupy and activate it. FPL is
structurally unrelated to DHPs and is thought to bind to a separate
site from DHPs on the ICa,L in a noncompetitive
manner (1, 4, 15, 16, 23). However, several reports
(11, 15, 22) suggest that there is an allosteric
interaction between the binding sites for FPL and DHPs even if they do
not share a common receptor on the channel protein itself. Thus there
is already evidence for interactions between binding sites in
ICa,L for the two classes of agonist.
In RyR2, it appears that FPL has more pronounced effects on single
channel activity than BAYK but does so only at higher drug concentrations. The fact that both agents activate the channel without
any effect by DHP antagonists (either directly on the channel or by
competitive interaction with DHP agonists) suggests that the RyR2 binds
only agonists and responds to binding with channel activation. It is
not yet clear whether agonist-binding sites on the two Ca2+
channels share structural similarities which could explain the efficacy
of both agonist types to modify channel function. We cannot be certain
as to the exact primary sequence on the RyR2 protein that might serve
as a docking region for these agonists. Comparisons between primary
structures of the ICa,L and the RyR2 show some
similarities, but because of the limited ability to extrapolate primary
structures to three-dimensional conformations, we must await additional
work to determine if there really is a shared binding site for
Ca2+ channel agonists on the two proteins. However,
physiological evidence suggests that there may be a binding site
specific for both classes of Ca2+ channel agonists on the
cardiac channel. This putative "Ca2+ channel agonist
receptor" is not present on RyR1 and may be partially occluded by
ancillary proteins such as triadin and/or junctin because the purified
cardiac channel (but not the crude channel) was activated by DHP
agonists. Additional experiments are required to determine why FPL
gains access to this receptor unimpeded in the crude channel and why
DHP agonists require stripping of these secondary proteins before
gaining access to the receptor.
| |
ACKNOWLEDGEMENTS |
|---|
This work was supported by National Heart, Lung, and Blood Institute Grant HL-30724 (to J. A. Wasserstrom), an American Heart Association Scientist Development Award (to A. J. Lokuta), and a medical student summer research fellowship from Northwestern University Medical School Division of Cardiology (to S. T. Reddy).
| |
FOOTNOTES |
|---|
Address for reprint requests and other correspondence: J. A. Wasserstrom, Div. of Cardiology, S203, Northwestern Univ. Medical School, 303 E. Chicago Ave., Chicago, IL 60611 (E-mail: ja-wasserstrom{at}northwestern.edu).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
10.1152/ajpheart.00788.2001
Received 4 September 2001; accepted in final form 1 April 2002.
| |
REFERENCES |
|---|
|
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
|
|---|
1.
Baxter, AJ,
Dixon J,
Ince F,
Manners CN,
and
Teague SJ.
Discovery and synthesis of methyl 2,5-dimethyl-4-[2-(phenylmethyl)benzoyl]-1H-pyrrole-3-carboxylate (FPL 64176) and analogues: the first examples of a new class of calcium channel activator.
J Med Chem
36:
2739-2744,
1993[ISI][Medline].
2.
Brown, L,
and
Erdmann E.
Binding of digitalis derivatives to beef, cat and human cardiac (Na+ + K+)-ATPase. Affinity and kinetic constants.
Arch Int Pharmacodyn Ther
271:
229-240,
1984[ISI][Medline].
3.
Fan, JS,
Yuan Y,
and
Palade P.
Kinetic effects of FPL 64176 on L-type Ca2+ channels in cardiac myocytes.
Naunyn Schmiedebergs Arch Pharm
361:
465-476,
2000[ISI][Medline].
4.
Ginap, T,
Dooley DJ,
and
Feuerstein TJ.
The non-dihydropyridine L-type voltage-sensitive calcium channel activator FPL 64176 enhances K+-evoked efflux of [3H]norepinephrine from rat neocortical slices.
Neurosci Lett
156:
35-38,
1993[ISI][Medline].
5.
Grabner, M,
Wang ZY,
Hering S,
Streissnig J,
and
Glossman H.
Transfer of 1,4-dihydroyridine sensitivity from L-type to class A (B1) calcium channels.
Neuron
16:
207-218,
1996[ISI][Medline].
6.
Hess, P,
Lansman JB,
and
Tsien RW.
Different mode of Ca channel gating behaviour favoured by dihydropyridine Ca agonists and antagonists.
Nature
311:
538-544,
1984[Medline].
7.
Hockerman, GH,
Peterson BZ,
Johnson BD,
and
Catterall WA.
Molecular determinants of drug binding and action on L-type calcium channels.
Annu Rev Pharmacol Toxicol
37:
361-396,
1997[ISI][Medline].
8.
Ito, M,
Hollander PB,
Marks BY,
and
Dutta S.
The effects of six cardiac glycosides on the transmembrane potential and contractile characteristics of the right ventricle of guinea pigs.
J Pharmacol Exp Ther
211:
561-570,
1970.
9.
Karagueuzian, HS,
and
Katzung BG.
Relative inotropic and arrhythmogenic effects of five cardiac steroids in ventricular myocardium: oscillatory afterpotentials and the role of endogenous catecholamines.
J Pharmacol Exp Ther
218:
348-356,
1981
10.
Katoh, H,
Scholtthauer K,
and
Bers DM.
Transmission of information from cardiac dihydropyridine receptor to ryanodine receptor: Evidence from BayK 8644 effects on resting Ca sparks.
Circ Res
87:
106-111,
2000
11.
Lauven, M,
Handrock R,
Muller A,
Hofmann F,
and
Herzig S.
Interaction of three structurally distinct Ca2+ channel activators with single L-type Ca2+ channels.
Naunyn Schmiedebergs Arch Pharm
360:
122-128,
1999[ISI][Medline].
12.
Pang, DC,
and
Sperelakis N.
Nifedipine, diltiazem, bepridil and verapamil uptakes into cardiac and smooth muscles.
Eur J Pharmacol
87:
199-207,
1983[ISI][Medline].
13.
Peterson, BX,
Tanada TN,
and
Catterall WA.
Molecular determinants of high affinity dihydropyridine binding in L-type calcium channels.
J Biol Chem
271:
5293-5296,
1996
14.
Rampe, D,
Anderson B,
Rapien-Pryor V,
Li T,
and
Dage RC.
Comparison of the in vitro and in vivo cardiovascular effects of two structurally distinct Ca++ channel activators, BAY K 8644 and FPL 64176.
J Pharmacol Exp Ther
265:
1125-1130,
1993
15.
Rampe, D,
and
Dage RC.
Funtional interactions between two Ca2+ channel activators, (S)-BAY K 8644 and FPL 64176, in smooth muscle.
Mol Pharmacol
41:
599-602,
1992[Abstract].
16.
Rampe, D,
and
Lacerda AE.
A new site for the activation of cardiac calcium channels defined by the nondihydropyridine FPL 64176.
J Pharmacol Exp Ther
259:
982-987,
1991
17.
Rousseau, E,
and
Meissner G.
Single cardiac sarcoplasmic reticulum Ca2+-release channel: activation by caffeine.
Am J Physiol Heart Circ Physiol
256:
H328-H333,
1989
18.
Sagawa, T,
Sagawa K,
Kelly JE,
Lokuta A,
Tsai J,
Kan E,
and
Wasserstrom JA.
Activation of purified cardiac ryanodine receptors by dihydropyridine agonists.
Am J Physiol Heart Circ Physiol
280:
H1201-H1207,
2001
19.
Schuster, A,
Lacinova L,
Klugbauer N,
Ito H,
Birnbaumer L,
and
Hofman F.
The IVS6 segment of the L-type calcium channel is critical for the action of dihydropyridines and phenylalkylamines.
EMBO J
15:
2365-2370,
1996[ISI][Medline].
20.
Tang, S,
Yatani A,
Bahinski A,
Mori Y,
and
Schwartz A.
Molecular localization of regions in the L-type calcium channel critical for dihyrdopyridine action.
Neuron
11:
1103-1121,
1993.
21.
Tsushima, RG,
Kelly JE,
and
Wasserstrom JA.
Characteristics of cocaine block of purified cardiac sarcoplasmic reticulum calcium release channels.
Biophys J
70:
1263-1274,
1996[Medline].
22.
Yamaguchi, S,
Okamura Y,
Nagao T,
and
Adachi-Akahane S.
Serine residue in the IIIS5-S6 linker of the L-type Ca2+channel 1C subunit is the critical determinant of the action of dihydropyridine Ca2+ channel agonists.
J Biol Chem
275:
41504-41511,
2000
23.
Zheng, W,
Rampe D,
and
Triggle DJ.
Pharmacological, radioligand binding and electrophysiological characteristics of FPL 64176, a novel nondihydropyridine Ca2+ channel activator, in cardiac and vascular preparations.
Mol Pharmacol
40:
734-741,
1991[Abstract].
This article has been cited by other articles:
|
|
 |
|
  J. A. Copello, A. V. Zima, P. L. Diaz-Sylvester, M. Fill, and L. A. Blatter Ca2+ entry-independent effects of L-type Ca2+ channel modulators on Ca2+ sparks in ventricular myocytes Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2129 - C2140. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
