INTRODUCTION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

CARDIAC ARRHYTHMIAS arise from abnormalities of either impulse propagation (reentry based) or impulse initiation (focal or ectopic). Although the development of reentry arrhythmias, which involves rotation of an excitation wave around an anatomic or functional block, is conceptually well understood (19, 34, 44), it remains unclear exactly how the excitation propagates from an ectopic cell into the surrounding cell network. Previous studies have addressed several important questions about ectopic activity associated with ischemia-reperfusion. Specifically, many electrophysiological studies have shown the ability of individual myocytes to exhibit either triggered activity or abnormal automaticity upon reperfusion and have tied such arrhythmic behavior to specific ionic currents (7, 11, 54). Studies conducted in isolated papillary muscles and Purkinje fibers have revealed the importance of electrotonic interactions across inexcitable regions of tissue in modulating the activity of ectopic foci (1) and the role of entrance block with exit conduction (protected ectopic foci) for depolarization-induced automaticity to spread (31, 41). Mapping studies conducted in intact hearts have revealed that 25% and 75% of arrhythmias occurring during ischemia and reperfusion, respectively, can be traced to ectopic sources (37), with the majority located close to the ischemic border zone (20). Finally, theoretical studies have addressed the critical size of an automatic focus, the safety factor (ratio of generated charge and that required to excite neighboring cells), and the roles of cell-to-cell coupling and anisotropic conduction in the propagation of ectopic activity (21, 43, 49). Despite these significant advancements, the actual progression of ectopic activity to arrhythmia has yet to be observed, mainly because the ischemic border of intact cardiac tissue has been inaccessible to real-time observation on a cellular scale. To visualize such a progression, we used a cellular model of ischemia-reperfusion that was recently developed in our laboratory (2). The results we obtained with this model not only have substantiated earlier theoretical estimates of the critical size of ectopic loci and the role of uncoupling in the generation of ectopic activity, but have also revealed a new phenomenon: a transient functional layer that is capable of sustaining autonomous ectopic waves.

	MATERIALS AND METHODS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Myocyte cultures. Cardiomyocytes from 1- and 2-day-old Sprague-Dawley rats were obtained by an enzymatic digestion procedure described previously (2). The cells were plated on 25-mm laminin-coated glass coverslips (10⁵ cells/cm²) and kept under standard culture conditions in DMEM, supplemented with 5% fetal bovine serum (FBS), 10 U/ml penicillin, 10 µg/ml gentamicin, and 1 µg/ml streptomycin. By the third day in culture, the cells had formed interconnected confluent networks that exhibited rhythmic, spontaneous contractions. The cells were used in experiments for the next 3-4 days.

Intracellular calcium measurements. Cells were loaded for 1 h with 5 µM fluo 4-AM in Tyrode solution at room temperature. The superfusion solutions contained 0.25 µM fluo 4-AM to maintain the intracellular dye concentration during extended experiments. Each spontaneous or stimulated action potential was associated with a transient increase in intracellular calcium (Ca), which we observed as a fluo 4 transient. To estimate diastolic slope values, we calculated Ca using the following equation (17): Ca = K_dR/(K_d/[Ca]  R + 1), where R is the normalized fluorescence (F/F₀), F₀ is the fluo 4 fluorescence at rest, and K_d is the dissociation constant for the Ca²⁺-fluo 4 complex. A value of 0.345 µM was assumed for the K_d (15), and resting Ca (Ca) was assumed to be 100 nM (10). The rate of change of Ca during 0.5 s preceding the fast rising phase of the Ca transient (CaT) was determined by linear regression. To visualize the propagation of ectopic activity, we recorded CaT instead of membrane potentials due to the following technical reasons. First, the closed design of the local injury chamber, which was essential for the temporal and spatial stability of the injury zone (I-zone) (2), precluded direct access by an electrode. Second, voltage-sensitive dyes exhibit less than a 10% change of emission intensity in response to an action potential (compared with a 10-fold increase in fluo 4 intensity associated with a CaT). This limited dynamic range renders potentiometric indicators unsuitable for experiments that require low magnification objectives to visualize the unpredictable occurrence of ectopic foci in a two-dimensional multicellular network, because these objectives collect only a limited amount of emitted light due to their low numerical apertures. In addition, rapid photobleaching of potentiometric dyes makes them inappropriate for extended experiments (40).

Experimental chamber. The experimental chamber consisted of a stainless steel holder for mounting a glass coverslip and a Plexiglas base that contained two inlets and one outlet (2). Inflow rates for the injury (I-solution) and control solutions (C-solution) were 30 and 75 µl/min, respectively. Two platinum electrodes embedded in the top of the chamber were used to stimulate a small cluster of myocytes immediately below. This stimulation initiated a wave of CaT that spread throughout the rest of the network with a propagation velocity of 6-9 cm/s. To determine the excitation threshold, we applied monophasic 1.2-ms pulses starting at 0.4 V/cm and then increased the stimuli in 0.1-V increments until each pacing pulse was followed by a CaT wave. The monolayer, which behaved as a syncytium, was then continuously paced at 0.2-0.5 Hz by a voltage 20% higher than the excitation threshold (average threshold values were 0.8 V/cm). Although we were able to observe reperfusion arrhythmias at 37°C, the temporal resolution of CaT recordings decreased substantially at this temperature. Consequently, we conducted the experiments at 25°C by mounting the stainless steel holder in a microincubator (Medical Systems) equipped with a Peltier controller, which provided a constant temperature inside the chamber. We chose not to employ a lower temperature, because of a decrease in gap junctional permeability, which occurs at temperatures below 20°C (8).

Experimental protocols. All solutions, including the I-solution, were equilibrated with atmospheric oxygen. The experiments were conduced in either spontaneously beating or paced (if intrinsic frequency was <0.1 Hz) monolayers. Initially, cells were superfused for 10-20 min with a control Tyrode solution containing (in mM) 136 NaCl, 0.8 MgCl₂, 4.0 KCl, 1.2 CaCl₂, 5.6 glucose, and 10 HEPES; pH 7.3. Subsequently, within 15 s after the onset of I-solution, an I-zone was created as described previously (2). This I-zone was surrounded by a control zone (C-zone), which was composed of myocytes superfused by control Tyrode solution. Washout with the control Tyrode solution constituted "reperfusion." The "microreperfusion" protocol was accomplished by substituting a washout step with increased relative inflow rates for the C- and I-solutions. Specifically, flow from a 5-ml syringe was added in parallel to flow from the control 30-ml syringe. This increased the flow of C-solution from 75 to 90 µl/min (while inflow of the I-solution remained the same) and effectively reduced the size of the I-zone.

Injury solution. The terms "ischemic" or "injury" environment in our study refer to a solution that reproduces certain elements of the extracellular milieu of ischemic cells (2, 48). It consisted of (in mM) 136 NaCl, 0.8 MgCl₂, 8 KCl, 1.2 CaCl₂, 20 deoxyglucose, 2 heptanol, 5 HEPES, and 5 MES; pH 6.5. Inclusion of the gap junction uncoupler heptanol allowed us to mimic the uncoupling effects of free fatty acids, e.g., arachidonic and palmitoleic acids, which have been shown to accumulate in the extracellular milieu of ischemic tissue (12, 38). In neonatal rat cardiomyocytes, 2 mM heptanol reversibly inhibits gap junction permeability (6, 46), whereas it's effect on Ca and spontaneous contractions is negligible (4, 23).

Acquisition systems. Fluo 4-loaded cells were imaged with low power magnification objectives (Olympus PlanApo ×2/0.08 numerical aperture and UPlanFl ×10/0.3 numerical aperture) to capture the I- and C-zones simultaneously. For the experiments shown in Figs. 1, 2, 4, and 5, the BioRad MRC 1024 confocal system was used. Experiments presented in Fig. 3 were acquired with a Metafluor Imaging System equipped with an intensified charge-coupled device camera (Pentamax, Princeton Instruments).

View larger version (77K): [in this window] [in a new window]   Fig. 1.   Generation of ectopic beats in the border zone. A: diagram on the left shows the experimental field and the injury zone (I-zone). The I-zone (shown in gray) was created by flows of control and ischemia-like solutions using a custom-designed superfusion chamber (2). Recordings of calcium transients (CaTs) were obtained from the border zone, inside the blue box shown on the left. The pseudocolor reflects increasing calcium concentrations as measured by fluo 4 fluorescence. a, Image was obtained during application of injury (I) solution. It shows a propagating wave of CaTs (CaT wave) that travels through the control area but does not penetrate into the I-zone. Black arrows point in the direction of CaT wave propagation. b, Appearance of ectopic clusters upon initiation of reperfusion. Three large clusters and a small one can be seen in this frame. c, Spreading of ectopic activity into surrounding network. Black arrows point in the direction of CaT wave propagation. B: A, b was used to plot the intensity profiles for regions where ectopic activity was observed. Two rectangular regions with their corresponding profiles are shown on the left. The position of these regions relative to the rest of the field can be seen on the right. The white dotted line marks the border of the I-zone.

View larger version (58K): [in this window] [in a new window]   Fig. 2.   Progression of ectopic CaTs into arrhythmic episodes. A: diagram of the experimental field. Recordings from the border area inside the blue box are shown in B and were used to create the isochronal maps in C. B: initial stages of propagation of ectopic activity. Three consecutive images show the initial stages of ectopic cluster expansion. Elapsed time (in s) is shown in the boxes. The pseudocolor reflects increasing calcium concentrations. C: isochronal maps illustrating the growth and spread of ectopic activity. Each isochronal map is composed of several sequential frames (110 ms between frames) with the areas of increased intracellular calcium (Ca) depicted in color. The colors correspond to the times shown on the right. The isochronal map on the left shows expansion of the ectopic activity presented in B. At that time (~219 s), the activity failed to spread into the surrounding cell network, and the event presented in this isochronal map is reflected as a distinct ectopic beat by the corresponding CaT trace. The following ectopic beats, including the one illustrated on the right isochronal map, propagated into the control network, causing an arrhythmia. D: CaTs collected from the control and ischemic areas (black and red squares in C). Dotted lines indicate timing of pacing stimuli. Initial 10 s of the recording shows that, during local ischemia, cells within the control network (black trace) exhibited regular CaTs, whereas cells within the I-zone were silent (red trace). Upon reperfusion, ectopic activity from the border zone (red trace, with arrows pointing to the individual beats) spread into the control network, causing an arrhythmia. * Additional peaks on the control trace between pacing stimuli, which were caused by the spread of ectopic activity from the border zone. Shaded regions correspond with the isochronal maps shown in C. AUF, arbitrary units of fluorescence.

View larger version (50K): [in this window] [in a new window]   Fig. 3.   Changes in diastolic Ca associated with ectopic activity. A: two traces from control (black) and border (red) zones illustrating an entire tachyarrhythmic episode are shown. Reperfusion was initiated at 550 s. The black arrow points to the first ectopic beat. B: relative position of the five regions of interest that used to acquire the traces on the right. The traces (shown in corresponding colors) reveal individual CaTs during the initial and late stages of the tachyarrythmic episode. The arrows point to the respective timing of the selected intervals. The red arrows point to a positive slope in diastolic Ca observed in ectopic regions. C: the averaged diastolic slope of CaTs from the ectopic regions (red column) was significantly greater (P = 0.003) than that from the control zone (black column). Fluo 4 fluorescence was converted to Ca, as described in MATERIALS AND METHODS, to calculate these slopes.

Data analysis. Unless specified otherwise, the conclusions of this study were based on the analysis of over 300 injury/reperfusion episodes (yield from a single litter was sufficient to plate 20-40 coverslips, which were then used for individual experiments). Specifically, in 190 experiments with the I-solution containing heptanol, the following responses were obtained: 1) no changes (immediate return to the original frequency), 46 cases (24%); and 2) tachyarrhythmias, 144 cases (76%). Tachyarrhythmias, in turn, included 1) tachycardia (monotonic increase in frequency), 57 cases (30%); 2) arrhythmia (irregular increase in frequency with observable ectopic beats), 63 cases (33%); and 3) tachyarrhythmia associated with the formation of spiral waves, 24 cases (13%). Spirals and other spatiotemporal patterns, which formed as a result of colliding ectopic waves, will be the subject of a followup paper and thus are not discussed here. In the 120 experiments that employed heptanol-free I-solution, tachycardia was observed 13 times (11%) and arrhythmia with the ectopic extra beats was observed 6 times (5%). The presented figures are typical results of corresponding scenarios. Quantitative results are expressed as means ± SD. Data and images were plotted using Microcal Origin 6.0 and Scion (NIH) Image software.

Chemicals. Collagenase type II was obtained from Worthington (Freehold, NJ). Media and porcine trypsin were obtained from GIBCO-BRL (Grand Island, NY). Fluo 4-AM was purchased from Molecular Probes (Eugene, OR). FBS and all other chemicals were purchased from Sigma (St. Louis, MO).

	RESULTS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Appearance of ectopic clusters. During "ischemia," the amplitude of CaTs within the I-zone declined progressively and was followed by cessation of cell beating. Although changes in CaT frequency inside and outside the I-zone were not detected during perfusion with I-solution, tachyarrhythmic episodes lasting 15-200 s were recorded in both the C- and I-zones immediately upon restoration of control flow in ~76% of the experiments. When these tachyarrhythmic episodes occurred, we observed ectopic CaTs within the former I-zone, predominantly within or close to the border (Fig. 1). Such ectopic activity appeared as a local increase in Ca with the amplitude and duration similar to pacing-elicited CaTs (Figs. 1 and 2). The average distance from the center of the functional border (defined in Ref. 2) to an ectopic cluster was 232 ± 113 µm. The linear sizes of the observed ectopic clusters varied from 180 to 300 µm (236 ± 57 µm), encompassing ~2-9 × 10⁴ µm² (shape of ectopic clusters depended on particular cellular arrangement, see examples in Figs. 1 and 2). This area corresponds to ~8-50 cells, which is in agreement with theoretical studies that estimate the minimum size of the ectopic region as 8-10 cells (52). It is possible, however, that these numbers are overestimates, because high binning, which was used to acquire the images, lowers spatial resolution of images to 44 µm/pixel (×2 objective) and makes clusters smaller than ~100 µm indistinguishable from noise. The fate of ectopic clusters was threefold: 1) activity remained confined to the ectopic cluster and, after 1-3 ectopic beats, this area was "swept" by a CaT wave from another source; 2) the cluster slowly expanded, encompassing a larger area of border or I-zone cells (Fig. 2, B and C) but failed to propagate into the control network; and 3) slow (0.1-0.4 cm/s) expansion of the ectopic cluster caused excitation of the surrounding network with fast propagation velocities (Fig. 2, C and D). As a result of the above events, a complex pattern of colliding ectopic waves spread through the entire field, including the former I- and C-zones. The exact patterns varied between experiments.

Ectopic activity is associated with changes in CaT shape. To understand the basis of the elevated excitability of ectopic regions, we examined CaTs in more detail using the MetaFluor imaging system (Fig. 3). Specifically, recordings were obtained from the border area and included CaTs from both ectopic and control regions. An immediate increase of baseline Ca was observed in myocytes from the ectopic regions upon reperfusion (Fig. 3, red trace). In this particular experiment, the first ectopic beat (black arrow) failed to propagate to neighboring myocytes, but the next CaT from the same ectopic locus drove the cell network for at least 140 s, until the pace approached the initial frequency of the layer. The slowing of ectopic pacing during the later stages of reperfusion revealed an important feature of the ectopic cells' CaT, a prominent rise in diastolic Ca (Fig. 3, red arrows), which resembled the diastolic depolarization of a pacemaker cell. Although only an estimate of Ca can be obtained from nonratiometric calcium probes, such as fluo 4, we calculated slope values for the control and ectopic CaTs using the K_d value of 345 nM (15, 17). The slope in ectopic regions ranged from 7 to 40 nM/s and was significantly higher than in control traces (Fig. 3C). This slope disappeared upon return of the original pacing pattern, suggesting its direct involvement in ectopic activity.

Role of cell-to-cell coupling and the number of injury events. Omission of heptanol from the I-solution sharply reduced the incidence of reperfusion arrhythmias (from 76% to 16%). Thus a 10-min superfusion with uncoupler-free I-solution resulted in the return of CaTs and restoration of rhythmicity across the entire field in a majority of the experiments. However, if the 10-min ischemia/10-min reperfusion cycle was repeated, the occurrence of reperfusion arrhythmias increased in proportion to the number of injury events (Fig. 4A). Typical traces from the C- and I-zones during the repetitive injury protocol are shown in Fig. 4B, and tachyarrhythmic episodes are marked with an asterisk. The lower traces in Fig. 4 reveal that tachyarrhythmic episodes in the C-zone occurred when cells inside the I-zone failed to exhibit CaTs upon reperfusion.

View larger version (37K): [in this window] [in a new window]   Fig. 4.   Repetitive injury increases the incidence of reperfusion arrhythmias. A: multiple brief superfusions with a heptanol-free ischemic solution increased the incidence of reperfusion arrhythmias in proportion to the number of injury events. Statistical data are from 35 experiments. B, top: continuous CaTs collected from the control zone (black) and I-zone (red) during six consecutive injury events, each consisting of 10 min of ischemia, followed by 10 min of reperfusion. The top traces are rather compressed and mainly illustrate changes in the relative amplitudes of CaTs. Acidic pH of the I-solution resulted in a downward shift of the fluo 4 baseline signal, due to its known pH dependence (28). The two insets below have an expanded time scale and reveal individual CaTs and changes in frequency. Specifically, the left inset shows that reperfusion after the second injury event recovers CaTs in the I-zone without changing the rhythmic control pattern. The right inset shows that reperfusion after the fifth injury event failed to restore CaTs inside the I-zone and induced an arrhythmia in the control zone. In this particular experiment, arrhythmic episodes occurred after the fourth, fifth, and sixth injury events (*).

Role of border zone propagation. Upon reperfusion, ectopic CaT often propagated along small areas of the border and then spread into the C- and recovering I-zones. In one series of experiments, we blocked the propagation of such activity into the inner area of the I-zone by continuing to superfuse it with I-solution (see MATERIALS AND METHODS and Fig. 5). Such "microreperfusion" allowed us to confine ectopic activity to the large segments of the border zone (Fig. 5A). Border zone propagation was followed by the escape of ectopic activity from the border zone into the surrounding control network, resulting in the generation of arrhythmias (Fig. 5B).

View larger version (113K): [in this window] [in a new window]   Fig. 5.   Border zone propagation. Consecutive frames illustrating the propagation of ectopic activity along the border zone during a microreperfusion protocol are shown. In this protocol, only a narrow, 300-µm width of border zone was reperfused with control Tyrode solution, whereas the remaining I-zone cells continued to be superfused with I-solution. A: microreperfusion caused the appearance of ectopic CaTs, which propagated alongside the border zone with an apparent speed of 0.1-0.4 cm/s. This sequence illustrates a wave of CaTs that receded without activating the surrounding control zone. B: spread of CaTs into the surrounding control network, where they were propagated at a faster velocity of 6-9 cm/s. A complete sequence of events can be seen in the online supplement.

	DISCUSSION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

Despite recent advances in mapping electrical activity in the heart (35, 45, 47, 55), cellular events within the border zone of an ischemic area have not been observed in situ. Thus until now the initial stages of ectopic activity and its propagation have been addressed mainly by computational approaches (16, 21, 52, 53). In contrast, this study provides insights from direct observation of ectopic activity, resulting from a new experimental approach developed in our laboratory (2). It employs a multicellular network of myocytes and fluo 4 fluorescence to visualize propagating ectopic waves of CaTs. Despite the absence of electrophysiological measurements, the results are highly relevant to cardiac arrhythmias. First, ultimately it is the disorganized contraction (as a result of CaT), not electrical activity, that renders the heart unable to pump blood efficiently. Second, monitoring CaTs as a means to measure beat rates and the velocity of impulse propagation has been used successfully both in vitro and in vivo (5, 14, 24). Third, CaTs measured in our experiments were direct indications of propagating action potentials, because the velocity of CaT wave propagation exceeded by at least two orders of magnitude the velocity of diffusion-based Ca waves (25, 27) and was similar to the velocity of action potentials measured by others in neonatal myocyte cultures (13, 29). In addition, the magnitude and velocity of ectopic CaTs that spread from the I-zone to the C-zone were identical to these elicited by the external pacing electrodes.

In our experimental protocol, the generation and propagation of ectopic activity occurred only during washout of the I-solution, i.e., during reperfusion (Figs. 1-3). What are the factors that promoted the generation of ectopic beats in these experiments? In the presence of I-solution, the gap junction conductance of myocyte within the I-zone is decreased due to the uncoupling effects of low pH (32, 51) and heptanol (4, 23, 46). Although upon reperfusion heptanol is rapidly removed from the cell surroundings (<15 s), intercellular coupling was restored only gradually. For example, Bastide et al. (4) found that gap junction conductance in neonatal rat myocyte pairs was restored to 90% of its control value ~90 s after withdrawal of heptanol. Therefore, during the first 100 s of reperfusion, a range of coupling conductances is superimposed upon rapidly recovering cell excitability, which passes through abnormally high values during the initial phase of reperfusion (7, 11, 33). Thus the combination of high excitability and decreased intercellular coupling creates conditions that favor the generation and propagation of ectopic activity from an individual myocyte or myocyte cluster (42). By decreasing cell-to-cell coupling, one effectively reduces a current "sink" leading to improvement of impulse propagation from an active focus (21, 50, 52). A similar phenomenon, a paradoxical improvement in the safety factor of conduction, was observed when cell-to-cell coupling was reduced in branching strands of cardiomyocytes (39). Our experiments provided two lines of evidence that decreased cell-to-cell coupling is essential for the successful progression of ectopic activity. First, omission of heptanol from the I-solution reduced the occurrence of reperfusion arrhythmias by more than fourfold (from 76% to 16%), suggesting that elevated excitability, alone, is insufficient for ectopic activity to proceed. Importantly, heptanol-containing control Tyrode solution failed to cause reperfusion arrhythmias (data not shown). The second line of evidence comes from our repetitive injury experiments (Fig. 4). Although CaTs recovered after a single exposure to heptanol-free I-solution, repetitive injury progressively diminished CaT amplitudes (Fig. 4B) and eventually led to cell death within the I-zone, as assessed with trypan blue (data not shown). At the same time, a strong correlation was observed between the incidence of reperfusion arrhythmias and the absence of CaTs in the I-zone upon reperfusion (Fig. 4A). Thus we hypothesize that the irreversible injury of I-zone myocytes, which is induced by repetitive ischemia-reperfusion cycles, removed the I-zone as an effective current "sink" and increased the probability of ectopic beat generation from the border zone. In other words, repetitive injury creates a unidirectional block for border zone cells, thereby increasing the safety factor for successful propagation of ectopic beats (49).

Whereas diminished coupling appears to be essential for both the generation and propagation of ectopic activity, an additional factor, increased cell excitability, is required for an ectopic cell or cluster to emerge. Direct visualization of ectopic sources in our cellular model of reperfusion-ischemia injury allowed us to simultaneously observe CaTs in ectopic and control regions. The distinguishing characteristic of the ectopic regions was a slow increase in diastolic Ca, which preceded the CaT and resembled the diastolic depolarization typical of pacemaker cells (Fig. 3). The basis of such an increase in diastolic Ca is unknown. It could be the consequence of reperfusion-induced alterations of ion channels and transporters or from reperfusion-induced Ca²⁺ overload (7, 9). The latter is known to occur in cardiac myocytes upon recovery from ischemia or acidosis and can cause triggered activity as a result of the inward sodium current generated by the Na⁺/Ca²⁺ exchanger or Ca²⁺-activated nonselective cation channels, facilitating myocyte depolarization. Ca²⁺ overload also enhances the probability of Ca²⁺ efflux from the sarcoplasmic reticulum (SR) during diastole, thereby increasing the likelihood of automaticity in latent pacemaker cells as well as ventricular and atrial myocytes (3). Through a positive feedback loop, which involves low-voltage-activated T-type Ca²⁺ channels and Ca²⁺-induced Ca²⁺ release from the SR, subsarcolemmal Ca²⁺ also may induce pacemaker activity in atrial cells (18, 26). Any of these mechanisms could be involved in the slow rise of diastolic Ca observed in our experiments.

In addition to the ionic basis of ectopic foci, it is important to understand how such activity is propagated. The fact that every myocyte within the recovering border zone was subjected to similar conditions in our experimental setup, and that multiple ectopic sites emerged upon reperfusion, suggest, that many, if not all, border cells are capable to exhibit ectopic activity. Thus assuming that the entire border zone area gains automaticity or triggered activity, we hypothesize that impulse propagation within such an area should follow the principles known for the sinoatrial (SA) node. In fact, theoretical studies have shown that thousands of pacemaker cells generate synchronized outgoing action potentials by an entrainment process in the SA node: after leading cell fires, an activation front propagates through the rest of the already entrained pacemakers with an apparent, slow conduction velocity (30). If such a tissue has homogeneous physiological properties, waves of action potentials generated from an ectopic source would be spread spherically. However, if the tissue surrounding the leading cell is not homogeneous with regard to either junction conductance or diastolic depolarization rate, the activation of neighboring cells would be asymmetric. Diastolic depolarization then acts as a "pulling" force, yielding in the extreme case a linear propagation. We observed such "linear" propagation along small (200-400 µm) regions of the border zone. The microreperfusion protocol allowed as to effectively "focus" ectopic activity in a narrow layer around the I-zone (Fig. 5).

Although we failed to observe ectopic activity during in vitro "ischemia," 25% of the arrhythmias that occur during ischemia in vivo can be attributed to ectopic sources (36). How can one explain this apparent paradox? We suggest that ischemic process in vivo could be associated with conditions, which are similar to those in the microreperfusion protocol. Specifically, it could be due to the dynamic conditions associated with an infarct area, when metabolic vasodilatation of adjacent coronary beds transiently relieves ischemic conditions in small areas of the infarct's border. Myocytes within such areas might then act as a source of ectopic arrhythmias, as we observed in our study. In other words, a microreperfusion-like process could be responsible for arrhythmias that occur during ischemia.

Whether initial development of ectopic activity occurred in small ectopic clusters during the original ischemia-reperfusion protocol or in an extended borderline pattern (microreperfusion protocol, Fig. 5), at this stage CaT spread with an apparent low velocity (0.1-0.4 cm/s). Such low velocities, nevertheless, exceed by at least order of magnitude the speed of Ca waves caused by Ca²⁺-induced Ca²⁺ release (22). On the other hand, the apparent velocities of CaTs in ectopic clusters were an order of magnitude slower than the propagation of CaTs through the control network (6-9 and 14-18 cm/s at 25 and 37°C, respectively). Thus we suggest that the slow velocities we observed in ectopic clusters were due to the entrainment mechanisms of conduction in a network of ectopically active cells, where the activation propagates with a slow apparent velocity (30).

On the basis of the results of the present study, one can extrapolate the concept of "border zone" propagation in a two-dimensional myocyte network to the three-dimensional myocardium by incorporating the following scenario of events. At the onset of global or partial reperfusion, reversibly injured cardiomyocytes form a transient functional layer over an infarcted, irreversibly injured area. Such a layer might consist of ectopically active but poorly coupled myocytes ("pacemaker-like layer") that are capable of sustaining autonomous ectopic waves. Upon restoration of cell-to-cell coupling, these ectopic waves would escape into the healthy network, generating arrhythmias. Given the significant differences in the electrical properties of cultured neonatal rat cardiomyocytes and adult human ventricular myocardium, additional experiments will be required to confirm occurrence and clinical relevance of such a scenario in vivo.

In summary, direct observation of reperfusion-induced ectopic activity in a two-dimensional network of neonatal rat cardiac myocytes corroborated theoretical predictions regarding the interplay between excitability and cell-to-cell coupling required for ectopic beat propagation. We have shown that an ectopic focus has to expand to a cell cluster of certain dimensions to activate the surrounding network. The experiments also revealed that CaTs in ectopic regions exhibit a slow rise during diastole and suggested that activity within an ectopic cluster propagates via a slow, entrainment-like process, similar to the propagation of action potentials in the SA node. Our results also suggest that, upon reperfusion, a functional ectopically active layer can be formed transiently over an injured area. Despite being surrounded by a normally conductive and excitable tissue, this layer, itself, can exhibit transient autonomous activity. These results may provide further insights into the mechanisms that underlie arrhythmias in the human myocardium.