HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via Web of Science (15)

Supplemental Videos

Supplemental Videos for figures 3, 5, 6, and 7e.

Files in this Data Supplement:

• Video 3 - A representative artery showing the effects of 10 mM [Mg²⁺]_O on [Ca²⁺]_CYT and myogenic tone (artery diameter) in a pressurized rat small mesenteric artery. Under control conditions, the artery generated myogenic tone, and many of the cells exhibited modest Fluo-4 fluorescence (directly related to [Ca²⁺]_CYT). When [Mg²⁺]_O was elevated to 10 mM at 50 sec, [Ca²⁺]_CYT (i.e., fluorescence) in each of the myocytes declined and the artery dilated (indicated by upward movement of the artery wall). Upon restoration of normal Krebs (with 1.2 mM Mg²⁺) at 160 sec, the [Ca²⁺]_CYT in the myocytes rose and the artery constricted.
• Video 5 - A representative artery showing the effects of 10 mM [Mg²⁺]_O on 75 mM K+-induced changes in [Ca²⁺]_CYT and vasoconstriction. Under control conditions, the artery generated myogenic tone, and many of the cells exhibited moderate Fluo-4 fluorescence. When [Mg²⁺]_O was elevated to 10 mM at 21 sec, [Ca²⁺]_CYT in each of the myocytes declined and the artery dilated. Introduction of 75 mM K+-Krebs in the continuous presence of high [Mg²⁺]_O at 106 sec induced a large increase in [Ca²⁺]_CYT associated with a marked vasoconstriction. When [Mg²⁺]_OO was restored to 1.2 mM at 219 sec, [Ca²⁺]_CYT again fell to a low level, and the artery recovered myogenic tone.
• Video 6 - A representative artery showing the effects of 0.3 µM Nifedipine (Nif) on 75 mM K⁺-induced changes in [Ca²⁺]_CYT and vasoconstriction. Under control conditions, the artery generated myogenic tone, and many of the cells exhibited modest Fluo-4 fluorescence. When 0.3 µM Nif was applied, at 9 sec, [Ca²⁺]_CYT in each of the myocytes declined and the artery dilated. Elevation of [K⁺]_O to 75 mM in the continued presence of Nif at 91 sec had little effect on [Ca²⁺]_CYT in most cells, but induced modest elevation of [Ca²⁺]_CYT in a few cells. There was no arterial wall displacement (i.e., vasconstriction) under these circumstances. Following washout of the Nif and high K⁺ at 158 sec, the myocyte fluorescence slowly increased and the artery recovered myogenic tone.
• Video 7e - A representative artery showing the effects of 10 mM [Mg²⁺]_O on 5 µM PE-induced changes in [Ca²⁺]_CYT (Fluo-4 fluorescence) and diameter. Introduction of 5 µM PE at 60 sec induced a large increase in [Ca²⁺]_CYT, which then settled down to a somewhat lower, but still elevated level. Superimposed on this were relatively large [Ca²⁺]_CYT fluctuations (oscillations) which occurred simultaneously in a number of cells. [Ca²⁺]_CYT changes were associated with a large and maintained vasoconstriction (despite a slowly declining [Ca²⁺]_CYT) and vasomotion; The constriction phase of the vasomotion occurred at the peaks of the [Ca²⁺]_CYT fluctuations. Elevation of [Mg²⁺]_O to 10 mM in the presence of PE markedly lowered [Ca²⁺]_CYT and abolished the fluctuations at 185 sec; this was accompanied by a marked vasodilation that was approaching PD. When [Mg²⁺]_O was restored to 1.2 mM at 245 sec. [Ca²⁺]_CYT promptly rose again, and began to oscillate; the artery reconstricted, and again exhibited vasomotion. Upon washout of the PE at 410 sec, [Ca²⁺]_CYT again fell to a low level, the [Ca²⁺]_CYT oscillations were abolished, and the artery dilated to PD.

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via Web of Science (15)