HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text (PDF) All Versions of this Article: 287/4/H1786    most recent 01143.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (16) Citing Articles via Google Scholar Google Scholar Articles by Okubo, S. Articles by Takekoshi, N. Search for Related Content PubMed PubMed Citation Articles by Okubo, S. Articles by Takekoshi, N.

Ischemic preconditioning and morphine attenuate myocardial apoptosis and infarction after ischemia-reperfusion in rabbits: role of -opioid receptor

¹Department of Cardiology, Kanazawa Medical University, Ishikawa 920-0293, Japan; and ²Division of Cardiology, Department of Medicine, Virginia Commonwealth University Medical Center, Richmond, Virginia 23298

Submitted 4 December 2003 ; accepted in final form 1 June 2004

	ABSTRACT

TOP ABSTRACT MATERIALS AND METHODS RESULTS DISCUSSION GRANTS REFERENCES

 
We examined whether ischemic preconditioning (IPC) attenuates ischemia-reperfusion injury, in part, by decreasing apoptosis and whether the -opioid receptor (DOR) plays a pivotal role in the regulation of apoptosis. Rabbits were subjected to 30-min coronary artery occlusion (CAO) and 180 min of reperfusion. IPC was elicited with four cycles of 5-min ischemia and 10-min reperfusion before CAO. Morphine (0.3 mg/kg iv) was given 15 min before CAO. Naloxone (Nal; 10 mg/kg iv) and naltrindole (Nti; 10 mg/kg iv), the respective nonselective and selective DOR antagonists were given 10 min before either morphine or IPC. Infarct size (%risk area) was reduced from 46 ± 3.8 in control to 11.6 ± 1.0 in IPC and 19.5 ± 3.8 in the morphine group (means ± SE; P < 0.001 vs. control). Nal blocked the protective effects of IPC and morphine, as shown by the increase in infarct size to 38.6 ± 7.2 and 44.5 ± 1.8, respectively. Similarly, Nti blocked IPC and morphine-induced protection. The percentage of apoptotic cells (revealed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay) decreased in IPC (3.6 ± 1.9) and morphine groups (5.2 ± 1.2) compared with control group (12.4 ± 1.6; P < 0.001). Nti pretreatment increased apoptotic cells 11.2 ± 2.2% in IPC and 12.1 ± 0.8% in morphine groups. Nal failed to block inhibition of apoptosis in the IPC group (% of cells: 5.7 ± 1.3 vs. 3.6 ± 1.9 in IPC alone; P > 0.05). These results were also confirmed by nucleosomal DNA laddering pattern. We conclude that IPC reduces lethal injury, in part, by decreasing apoptosis after ischemia-reperfusion and activation of the DOR may play a crucial role in IPC or morphine-induced myocardial protection.

reperfusion injury

Previous studies (2, 24, 30) have shown that ischemic preconditioning (IPC) markedly reduces necrosis in the heart. IPC also decreases internucleosomal DNA fragmentation in rat hearts in vivo, and there was a direct correlation between myocardial infarct size and DNA fragmentation after IPC (29). IPC has been shown to attenuate processing and activation of caspase-1 and caspase-3, and cleavage of poly(ADP-ribose) polymerase after prolonged ischemia and reperfusion in the heart (28). IPC is primarily mediated by activation of several triggers, including free radicals, adenosine, catecholamines, and bradykinin (reviewed in Ref. 26). In addition, studies from Gross's laboratory (13) have shown that opioids play an important role in IPC against myocardial infarction. The nonselective and selective opioid receptor antagonists, naloxone (Nal) and naltrindole (Nti), respectively, have been shown to completely block the infarct-limiting effect of IPC in rats (31), rabbits (21), and pigs (34). Similarly, the nonpeptide opioid agonist morphine mimicked IPC in the rat heart (32), which was abrogated by Nal, suggesting the role of opioid receptors in cardioprotection. Because opioid receptor signaling has been identified as the integral components of the cardioprotective effect of IPC (13), we hypothesized that this pathway may also regulate apoptosis during IPC. Accordingly, the major goal of this study was to show whether the inhibition of apoptosis and necrosis after IPC is mediated by activation of the -opioid receptor. A second goal was to show whether pharmacological preconditioning achieved by infusion of morphine also induces anti-apoptotic effect through -opioid receptor in the rabbit heart after I/R.

	MATERIALS AND METHODS

TOP ABSTRACT MATERIALS AND METHODS RESULTS DISCUSSION GRANTS REFERENCES

 
All procedures were performed in conformance with the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, Revised 1985).

Experimental groups and protocol. Ninety-two male New Zealand White rabbits (2.5–3.2 kg body wt) were used in this study. All rabbits underwent 30-min coronary artery occlusion (CAO) and 180 min of I/R, as shown in Fig. 1. The animals were randomized into the following eight groups: 1) control (n = 12), in which no treatment was given; 2) Nal control (n = 10), in which rabbits were treated with a nonspecific blocker of opioid receptor Nal (10 mg/kg iv) 10 min before I/R; 3) Nti control (n = 10), where rabbits were treated with a selective blocker of the -opioid receptor Nti (10 mg/kg iv) 10 min before I/R; 4) the preconditioning group (IPC; n = 12), where the IPC protocol consisted of four 5-min occlusions, each separated by 10-min reperfusion; 5) morphine (Mor, 0.3 mg/kg, n = 12) was injected at a dose of 0.3 mg/kg iv 15 min before sustained ischemia; 6) Nal+IPC (n = 12), where rabbits were treated with Nal (10 mg/kg iv) 10 min before IPC protocol (see group 4); 7) Nti+IPC (n = 12), where animals were given Nti (10 mg/kg iv) 10 min before IPC; and 8) Nti+Mor (n = 12). Nti (10 mg/kg iv) was given 10 min before morphine, which was injected (0.3 mg/kg iv) 15 min before sustained ischemia. In each group, four rabbits were used for evaluation of apoptosis.

View larger version (15K): [in this window] [in a new window]   Fig. 1. Experimental protocol showing ischemic preconditioning (IPC), ischemia-reperfusion, and treatment schedules of morphine (Mor) and other inhibitors of opioid receptors. Nal, naloxone; Nti, naltrindole.

Measurement of hemodynamics. The hemodynamic measurements included heart rate and mean arterial pressures. The rate-pressure product was determined as the product of the heart rate and peak arterial pressure. These parameters were continuously measured throughout the duration of the experimental protocol (Table 1).

Genomic DNA extraction and agarose gel electrophoresis. For determination of genomic DNA fragmentation, hearts were rapidly removed after I/R and tissue samples from the ischemic region were homogenized. DNAs were isolated by lysis in digestion buffer (75 mM NaCl, 25 mM EDTA, 10 mM Tris, pH 8.0, 1% SDS, and 0.4 mg/ml freshly added proteinase K). After overnight digestion, DNAs were phenol/chloroform extracted, precipitated, spooled onto glass rods, dried, and dissolved in 10 mM Tris and 1 mM EDTA, pH 8.0, and quantified with the use of a spectrophotometer. For determination of DNA fragmentation, we used ApoAlert LM-PCR Ladder assay kit (Clontech) according to a previously described method (36). Briefly, 6 µg genomic DNA was mixed with 1 nmol 24 base pairs (bp) and 1 nmol 12-bp unphosphorylated oligonucleotides, annealed, and ligated. Ligations were stored at –20°C until PCR was performed. PCR reactions used 150-ng ligated DNA and used the 24-bp oligonucleotide as a primer. Samples were amplified for 25 cycles of PCR, and PCR products were analyzed by electrophoresis on 3% agarose gel (16).

Exclusion criteria. Animals were excluded from the study due to the following reasons: 1) CAO did not produce adequate ischemia (the area at risk was <20% of the left ventricle); 2) intractable ventricular fibrillation occurred during ischemia and/or reperfusion; and 3) the animal died during the surgical procedure, so the protocol was not completed.

Statistical analysis. Values are expressed as means ± SE. The statistical significance was determined by ANOVA with repeated measurements. If a significant F value was found, Scheffé's test for multiple comparisons was used to identify differences among groups. Comparisons of means were made by using the Student's t-test for unpaired values. Statistical differences were considered significant if the P value was <0.05.

	RESULTS

TOP ABSTRACT MATERIALS AND METHODS RESULTS DISCUSSION GRANTS REFERENCES

 
Infarct size. The risk areas ranged from 50% to 56% with no significant differences between all the groups (Fig. 2A;P > 0.05). These data suggest that changes in the size of infarcts observed between various groups in our experiments were not related to the percentage of area of the left ventricle occluded by our technique. Infarct size (expressed as a percentage of risk area) was 11.6 ± 1.0 in the IPC group and 19.5 ± 3.8 in the morphine-treated group, both of which were significantly lower compared with the infarct size of 46 ± 3.8 in the control group (P < 0.001, means ± SE; Fig. 2B). This reduction in infarct size by IPC was abolished by pretreatment with the nonselective opioid receptor antagonist Nal (38.6 ± 7.2%) and the -opioid-specific antagonist Nti (44.5 ± 1.8%). The infarct-limiting effect of morphine was also abolished by pretreatment with Nti (Nti+Mor: 49.3 ± 1.7% vs. 19.5 ± 3.8% for morphine; P < 0.001). Treatment of control rabbits with Nti or Nal had infarct size of 41.5 ± 3.6% and 42 ± 4.5%, respectively, which were not different compared with the infarct size in the untreated control group (P > 0.05).

View larger version (52K): [in this window] [in a new window]   Fig. 2. Bar diagram showing risk area of the left ventricle (%LV; A) and infarct size (%risk area; B) for groups 1–8. See MATERIALS AND METHODS for a description of treatment groups. Results are means ± SE in 6–7 rabbits in each group (*P < 0.001).

View larger version (75K): [in this window] [in a new window]   Fig. 3. A: light photomicrographs of heart sections from control group of rabbits subjected to 30 min of ischemia and 3 h of reperfusion. The bright green dots (indicated by arrows) correspond to a representative terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL)-positive (fluorescent) nucleus. B: percentage of TUNEL-positive cells in heart cells. Data shown are means ± SE of four independent experiments quantified from five fields. The treatment groups and abbreviations are described in detail in MATERIALS AND METHODS. *P < 0.001.

View larger version (92K): [in this window] [in a new window]   Fig. 4. Ligation-mediated PCR analysis of DNA from the ischemic zone. Genomic DNAs were prepared from various experimental groups, mixed with unphosphorylated oligonucleotides, annealed, and ligated. The DNA samples were amplified and PCR products were analyzed by electrophoresis on 3% agarose gel. The data shown are representative of 5 separate experiments. M, marker lane. The treatment abbreviations are same as in the experimental protocol and described in detail in MATERIALS AND METHODS.

	DISCUSSION

TOP ABSTRACT MATERIALS AND METHODS RESULTS DISCUSSION GRANTS REFERENCES

 
The major findings of this study are summarized as follows. First, the infarct-limiting effect of IPC and morphine is significantly blocked by Nal and Nti, the nonselective and selective -opioid inhibitor antagonists, respectively. Second, both IPC and morphine caused a significant decrease in the TUNEL-positive cardiomyocytes, inhibited the fragmentation of genomic DNA in the ischemic zone, and reduced infarct size compared with the control hearts. Third, Nti treatment before IPC and morphine reversed the anti-apoptotic and infarct-limiting effect of IPC and morphine. On the other hand, Nal failed to block the antiapoptotic effect of IPC as well as morphine. Both Nal and Nti had no effect on infarct size or apoptosis in the control rabbits subjected to I/R. The hemodynamics remained largely unchanged among the groups during the I/R protocol. For the first time, our results show an impressive anti-apoptotic effect of IPC, which is selectively mediated by -opioid receptor in the intact rabbit heart.

Many previous studies (12, 18) support the evidence that apoptosis may be the predominant form of ischemia-related cell death in the heart. Also, it has been shown that internucleosomal DNA fragmentation is restricted in the infarcted area after prolonged I/R in rat hearts in vivo. Both DNA fragmentation and infarct size were significantly decreased after prolonged ischemia and reperfusion in the preconditioned rat hearts in vivo (29). Moreover, a direct correlation between myocardial infarct size and internucleosomal DNA fragmentations was reported (29). Consistent with these studies, our data also showed significant reduction of apoptotic cardiomyoctes after IPC in the ischemic zone, which was correlated with reduction of infarct size in the heart. An interesting observation in these studies is that selective -opioid receptor antagonist completely abolished the anti-apoptotic protection of IPC, suggesting an important role of this receptor in preventing cell death. Moreover, nonpeptide opioid, morphine also mimicked the cardioprotective effect of IPC, both in terms of infarct size reduction and attenuation of apoptosis. Morphine is considered a classic µ-opioid receptor agonist, although there is evidence that it also has important effects on the - and -opioid receptors and that cross-talk between µ- and -receptors can occur (4). The observation that morphine-mediated inhibition of apoptosis was also abolished by pretreatment with Nti further confirms the role of -opioid receptor in protecting against cardiomyocyte death. Interestingly, whereas Nal abolished the infarct-limiting effect of IPC, the drug failed to block anti-apoptotic effect of IPC or morphine. The reason for these discordant results remains unclear, but it is possible that Nal could selectively block the necrotic rather than the antiapoptotic pathway of acute IPC in the rabbit heart. Alternatively, perhaps different opioid receptors have opposing effects on apoptosis, thus blocking both by Nal may result in no significant effect.

Several previous studies by Gross and co-workers (see Ref. 13 for review) suggest the role of opioids in early IPC. These investigators demonstrated that the nonselective opioid receptor antagonist Nal completely antagonized the ability of IPC to reduce infarct size, whether administered before IPC stimulus or after the IPC stimulus just before the index ischemia. Subsequent studies by Gross's group showed that selective -1 antagonist 7-benzylidenenaltrexone blocked IPC-mediated protection in a dose-dependent fashion (33). Conversely, the selective -1 opioid receptor agonist TAN-67 produced a potent cardioprotective effect in chick embryonic myocytes, which was blocked by 7-benzylidenenaltrexone (15). In the present study, the dose of Nal or Nti (10 mg/kg) used in this study was higher compared with the one used in other studies used to block preconditioning (31, 32). However, none of these doses of the drugs had a significant effect on myocardial infarct size or apoptosis (in terms of TUNEL-positive nuclei or DNA fragmentation) compared with the nontreated control animals.

In the present study, we assessed apoptosis after a relatively short period of reperfusion (i.e., 3 h) by TUNEL and DNA fragmentation, which are known to measure the later stages of cell death. It has been reported (38) that the extent of necrosis peaks at 24 h of reperfusion, and remains constant thereafter. On the other hand, the appearance of apoptotic cells in the perinecrotic area progressively increases up to 72 h, suggesting that necrosis and apoptosis occur simultaneously during reperfusion, with a rapidly developing necrotic cell death during the early phase of reperfusion, followed by a slower appearance of apoptosis during the late phase of reperfusion. The number of apoptotic cells in the perinecrotic myocardium progressively increases during the extended period of reperfusion, which suggests the role of apoptosis in the development of infarction (39). Considering the reported delayed preconditioning effect of opioids (6), it would not be surprising if morphine or IPC also delay the extent of apoptosis in the survival animal models. This is an interesting question, which warrants investigations in the future.

It has been reported that the protective effect of opioids involves selective activation of signaling pathways, including PKC- (10), non-Src-dependent tyrosine kinase and ERK-1/2 (8, 9, 11). There are studies (7) that suggest the -1-opioid receptor reduced infarct size through the opening of mitochondrial ATP-sensitive K⁺ channels, the proposed effectors of IPC. Moreover, the pharmacological opening of mitochondrial ATP-sensitive K⁺ channels by diazoxide has been shown to preserve mitochondrial integrity and suppress the markers of apoptosis (1). It remains to be seen whether a similar signaling pathway also follows the -opioid receptor-mediated antiapoptotic effect of morphine and IPC. A recent study (20) showed that morphine protected against the death of primary rat neonatal astrocytes by nitric oxide and peroxynitrite. Pretreatment of astrocytes with phosphatidylinositol 3-kinase (PI3-kinase) inhibitors, including wortmannin and LY-294002, abrogated the effect of morphine on nitric oxide-induced cell death, indicating that the effects of morphine were, in part, mediated by PI3-kinase. It is possible that activation of -opioid receptor stimulation by IPC or morphine potentially activate the PI3-kinase pathway that may ultimately be responsible for the antiapoptotic effect of this receptor. There is currently no data in support of this contention, although the role of PI3-kinase in the acute phase of IPC against lethal ischemia-reperfusion injury has been well documented by recent published studies (23, 37).

In summary, our results provide the first direct evidence that both IPC and morphine attenuate cardiomycyte apoptosis through the activation of -opioid receptors. These observations raise the interesting possibility that novel agonists of -opioid receptors may be clinically useful in preventing cell death during I/R injury and heart failure and could potentially be used for treatment directed at these lethal disorders.

	GRANTS

TOP ABSTRACT MATERIALS AND METHODS RESULTS DISCUSSION GRANTS REFERENCES

 
This work was supported in part by Collaborative Research Funding Grant C2003-1 from Kanazawa Medical University (to S. Okubo) and by National Heart, Lung, and Blood Institute Grants HL-51045 and HL-59469 (to R. C. Kukreja).

	FOOTNOTES

 

Address for reprint requests and other correspondence: S. Okubo, Dept. of Cardiology, Kanazawa Medical Univ., 1-1 Daigaku, Uchinada, Kahoku, Ishikawa 920-0293, Japan (E-mail: shinkubo{at}kanazawa-med.ac.jp)

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

	REFERENCES

TOP ABSTRACT MATERIALS AND METHODS RESULTS DISCUSSION GRANTS REFERENCES

 

1. Akao M, Ohler A, O'Rourke B, and Marban E. Mitochondrial ATP-sensitive potassium channels inhibit apoptosis induced by oxidative stress in cardiac cells. Circ Res 88: 1267–1275, 2001.[Abstract/Free Full Text]
2. Anderson BL, Berry RW, and Telser A. A sodium dodecyl sulfate polyacrylamide gel electrophoresis system that separates peptides and proteins in the molecular weight range of 2,500 to 90,000. Anal Biochem 132: 365–375, 1983.[CrossRef][Web of Science][Medline]
3. Buerke M, Murohara T, Skurk C, Nuss C, Tomaselli K, and Lefer AM. Cardioprotective effect of insulin-like growth factor I in myocardial ischemia followed by reperfusion. Proc Natl Acad Sci USA 92: 8031–8035, 1995.[Abstract/Free Full Text]
4. Ela C, Barg J, Vogel Z, Hasin Y, and Eilam Y. Distinct components of morphine effects on cardiac myocytes are mediated by the - and -opioid receptors. J Mol Cell Cardiol 29: 711–720, 1997.[CrossRef][Web of Science][Medline]
5. Fliss H and Gattinger D. Apoptosis in ischemic and reperfused rat myocardium. Circ Res 79: 949–956, 1996.[Abstract/Free Full Text]
6. Fryer RM, Hsu AK, Eells JT, Nagase H, and Gross GJ. Opioid-induced second window of cardioprotection: potential role of mitochondrial K_ATP channels. Circ Res 84: 846–851, 1999.[Abstract/Free Full Text]
7. Fryer RM, Hsu AK, Nagase H, and Gross GJ. Opioid-sensitive cardioprotection against myocardial infarction and arrhythmias: mitochondrial versus sarcolemmal ATP-sensitive potassium channels. J Pharmacol Exp Ther 294: 451–457, 2000.[Abstract/Free Full Text]
8. Fryer RM, Pratt PF, Hsu AK, and Gross GJ. Differential activation of extracellular signal regulated kinase isoforms in preconditioning and opioid-induced cardioprotection. J Pharmacol Exp Ther 296: 647–654, 2001.
9. Fryer RM, Schultz JJ, Hsu AK, and Gross GJ. Pretreatment with tyrosine kinase inhibitors partially attenuates ischemic preconditioning in rat hearts. Am J Physiol Heart Circ Physiol 275: H2009–H2015, 1998.[Abstract/Free Full Text]
10. Fryer RM, Wang Y, Hsu AK, and Gross GJ. Essential activation of PKC- in opioid-initiated cardioprotection. Am J Physiol Heart Circ Physiol 280: H1346–H1353, 2001.[Abstract/Free Full Text]
11. Fryer RM, Wang Y, Hsu AK, Nagase H, and Gross GJ. Dependence of ₁-opioid receptor-induced cardioprotection on a tyrosine kinase-dependent but not a Src-dependent pathway. J Pharmacol Exp Ther 299: 477–482, 2001.[Abstract/Free Full Text]
12. Gottlieb RA, Burleson KO, Kloner RA, Babior BM, and Engler RL. Reperfusion injury induces apoptosis in rabbit cardiomyocytes. J Clin Invest 94: 1621–1628, 1994.[Web of Science][Medline]
13. Gross GJ. Role of opioids in acute and delayed preconditioning. J Mol Cell Cardiol 35: 709–718, 2003.[CrossRef][Web of Science][Medline]
14. Hamet P, Richard L, Dam TV, Teiger E, Orlov SN, Gaboury L, Gossard F, and Tremblay J. Apoptosis in target organs of hypertension. Hypertension 26: 642–648, 1995.[Abstract/Free Full Text]
15. Huh J, Gross GJ, Nagase H, and Liang BT. Protection of cardiac myocytes via ₁-opioid receptors, protein kinase C, and mitochondrial K_ATP channels. Am J Physiol Heart Circ Physiol 280: H377–H383, 2001.[Abstract/Free Full Text]
16. Ito K. DNA fragmentation of human infarcted myocardial cells demonstrated by the nick end labeling method and DNA agarose gel electrophoresis. Am J Pathol 146: 1325–1331, 1995.[Abstract]
17. Itoh G, Tamura J, Suzuki M, Suzuki Y, Ikeda H, Koike M, Nomura M, Jie T, and Ito K. DNA fragmentation of human infarcted myocardial cells demonstrated by the nick end labeling method and DNA agarose gel electrophoresis. Am J Pathol 146: 1325–1331, 1995.[Abstract]
18. Kajstura J, Cheng W, Reiss K, Clark WA, Sonnenblick EH, Krajewski S, Reed JC, Olivetti G, and Anversa P. Apoptotic and necrotic myocyte cell deaths are independent contributing variables of infarct size in rats. Lab Invest 74: 86–107, 1996.[Web of Science][Medline]
19. Kajstura J, Mansukhani M, Cheng W, Reiss K, Krajewski S, Reed JC, Quaini F, Sonnenblick EH, and Anversa P. Programmed cell death and expression of the protooncogene bcl-2 in myocytes during postnatal maturation of the heart. Exp Cell Res 219: 110–121, 1995.[CrossRef][Web of Science][Medline]
20. Kim MS, Cheong YP, So HS, Lee KM, Kim TY, Oh J, Chung YT, Son Y, Kim BR, and Park R. Protective effects of morphine in peroxynitrite-induced apoptosis of primary rat neonatal astrocytes: potential involvement of G protein and phosphatidylinositol 3-kinase (PI3-kinase). Biochem Pharmacol 61: 779–786, 2001.[CrossRef][Web of Science][Medline]
21. Kuzume K, Wolff RA, Amakawa K, Kuzume K, and Van Winkle DM. Sustained exogenous administration of Met(5)-enkephalin protects against infarction in vivo. Am J Physiol Heart Circ Physiol 285: H2463–H2470, 2003.[Abstract/Free Full Text]
22. Liu Y, Cigola E, Cheng W, Kajstura J, Olivetti G, Hintze TH, and Anversa P. Myocyte nuclear mitotic division and programmed myocyte cell death characterize the cardiac myopathy induced by rapid ventricular pacing in dogs. Lab Invest 73: 771–787, 1995.[Web of Science][Medline]
23. Mocanu MM, Bell RM, and Yellon DM. PI3-kinase and not p42/p44 appears to be implicated in the protection conferred by ischemic preconditioning. J Mol Cell Cardiol 34: 661–668, 2002.[CrossRef][Web of Science][Medline]
24. Murry CE, Jennings RB, and Reimer KA. Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium. Circulation 74: 1124–1136, 1986.[Abstract/Free Full Text]
25. Okubo S, Bernardo NL, Elliott GT, Hess ML, and Kukreja RC. Tyrosine kinase signaling in action potential shortening and expression of HSP72 in late preconditioning. Am J Physiol Heart Circ Physiol 279: H2269–H2276, 2000.[Abstract/Free Full Text]
26. Okubo S, Bernardo NL, Yoshida K, and Kukreja RC. Myocardial preconditioning: basic concepts and potential mechanisms. Mol Cell Biochem 196: 3–12, 1999.[CrossRef][Web of Science][Medline]
27. Okubo S, Wildner O, Shah MR, Chelliah JC, Hess ML, and Kukreja RC. Gene transfer of heat-shock protein 70 reduces infarct size in vivo after ischemia/reperfusion in the rabbit heart. Circulation 103: 877–881, 2001.[Abstract/Free Full Text]
28. Piot CA, Martini JF, Bui SK, and Wolfe CL. Ischemic preconditioning attenuates ischemia/reperfusion-induced activation of caspases and subsequent cleavage of poly(ADP-ribose) polymerase in rat hearts in vivo. Cardiovasc Res 44: 536–542, 1999.[Abstract/Free Full Text]
29. Piot CA, Padmanaban D, Ursell PC, Sievers RE, and Wolfe CL. Ischemic preconditioning decreases apoptosis in rat hearts in vivo. Circulation 96: 1598–1604, 1997.[Abstract/Free Full Text]
30. Schultz JEJ, Qian YZ, Gross GJ, and Kukreja RC. The ischemia-selective K_ATP channel antagonist, 5-hydroxydecanoate, blocks ischemic preconditioning in the rat heart. J Mol Cell Cardiol 29: 1055–1060, 1997.[CrossRef][Web of Science][Medline]
31. Schultz JEJ, Rose E, Yao Z, and Gross GJ. Evidence for involvement of opioid receptors in ischemic preconditioning in rat heart. Am J Physiol Heart Circ Physiol 268: H2157–H2161, 1995.[Abstract/Free Full Text]
32. Schultz JJ, Hsu A, and Gross GJ. Ischemic preconditioning and morphine-induced cardioprotection involve the -opioid receptor in the intact rat heart. J Mol Cell Cardiol 29: 2187–2195, 1997.[CrossRef][Web of Science][Medline]
33. Schultz JJ, Hsu AK, and Gross GJ. Ischemic preconditioning in the intact rat heart is mediated by ₁- but not µ- or -opioid receptors. Circulation 97: 1282–1289, 1998.[Abstract/Free Full Text]
34. Schulz R, Gres P, and Heusch G. Role of endogenous opioids in ischemic preconditioning but not in short-term hibernation in pigs. Am J Physiol Heart Circ Physiol 280: H2175–H2181, 2001.[Abstract/Free Full Text]
35. Sharov VG, Sabbah HN, Shimoyama H, Goussev AV, Lesch M, and Goldstein S. Evidence of cardiocyte apoptosis in myocardium of dogs with chronic heart failure. Am J Pathol 148: 141–149, 1996.[Abstract]
36. Staley K, Blaschke AJ, and Chun J. Apoptosis DNA fragmentation is detected by a semiquantitative ligation-mediated PCR of blunt DNA ends. Cell Death Differ 4: 66–75, 1997.[CrossRef][Web of Science][Medline]
37. Tong H, Chen W, Steenbergen C, and Murphy E. Ischemic preconditioning activates phosphatidylinositol-3-kinase upstream of protein kinase C. Circ Res 87: 309–315, 2000.[Abstract/Free Full Text]
38. Zhao ZQ, Nakamura M, Wang NP, Velez DA, Hewan-Lowe KO, Guyton RA, and Vinten-Johansen J. Dynamic progression of contractile and endothelial dysfunction and infarct extension in the late phase of reperfusion. J Surg Res 94: 133–144, 2000.[CrossRef][Web of Science][Medline]
39. Zhao ZQ, Velez DA, Wang NP, Hewan-Lowe KO, Nakamura M, Guyton RA, and Vinten-Johansen J. Progressively developed myocardial apoptotic cell death during late phase of reperfusion. Apoptosis 6: 279–290, 2001.[CrossRef][Web of Science][Medline]

This article has been cited by other articles:

				A. J. Zatta, H. Kin, D. Yoshishige, R. Jiang, N. Wang, J. G. Reeves, J. Mykytenko, R. A. Guyton, Z.-Q. Zhao, J. L. Caffrey, et al. Evidence that cardioprotection by postconditioning involves preservation of myocardial opioid content and selective opioid receptor activation Am J Physiol Heart Circ Physiol, March 1, 2008; 294(3): H1444 - H1451. [Abstract] [Full Text] [PDF]

				L.-L. Yao, Y.-G. Wang, W.-J. Cai, T. Yao, and Y.-C. Zhu Survivin mediates the anti-apoptotic effect of {delta}-opioid receptor stimulation in cardiomyocytes J. Cell Sci., March 1, 2007; 120(5): 895 - 907. [Abstract] [Full Text] [PDF]

				M. A. Lessa and E. Tibirica Pharmacologic Evidence for the Involvement of Central and Peripheral Opioid Receptors in the Cardioprotective Effects of Fentanyl Anesth. Analg., October 1, 2006; 103(4): 815 - 821. [Abstract] [Full Text] [PDF]

				D.J. Kim, D.I. Kim, S.K. Lee, S.H. Suh, Y.J. Lee, J. Kim, T.S. Chung, and J.E. Lee Protective effect of agmatine on a reperfusion model after transient cerebral ischemia: Temporal evolution on perfusion MR imaging and histopathologic findings. AJNR Am. J. Neuroradiol., April 1, 2006; 27(4): 780 - 785. [Abstract] [Full Text] [PDF]

				J. Weil, O. Zolk, J. Griepentrog, U. Wenzel, W. H. Zimmermann, and T. Eschenhagen Alterations of the preproenkephalin system in cardiac hypertrophy and its role in atrioventricular conduction Cardiovasc Res, February 1, 2006; 69(2): 412 - 422. [Abstract] [Full Text] [PDF]

				D. Weihrauch, J. G. Krolikowski, M. Bienengraeber, J. R. Kersten, D. C. Warltier, and P. S. Pagel Morphine Enhances Isoflurane-Induced Postconditioning Against Myocardial Infarction: The Role of Phosphatidylinositol-3-Kinase and Opioid Receptors in Rabbits Anesth. Analg., October 1, 2005; 101(4): 942 - 949. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) All Versions of this Article: 287/4/H1786    most recent 01143.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (16) Citing Articles via Google Scholar Google Scholar Articles by Okubo, S. Articles by Takekoshi, N. Search for Related Content PubMed PubMed Citation Articles by Okubo, S. Articles by Takekoshi, N.