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Am J Physiol Heart Circ Physiol 287: H2478-H2486, 2004. First published July 29, 2004; doi:10.1152/ajpheart.00217.2004
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Functional effects of enhancing or silencing adenosine A2b receptors in cardiac fibroblasts

Yinghong Chen,1 Sara Epperson,1 Lala Makhsudova,1 Bruce Ito,2 Jorge Suarez,1 Wolfgang Dillmann,1 and Francisco Villarreal1

1Department of Medicine, University of California, San Diego 92103-8412; and 2Metabasis Therapeutics Incorporated, San Diego, California 92121

Submitted 10 March 2004 ; accepted in final form 26 July 2004


   ABSTRACT
TOP
 ABSTRACT
MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 GRANTS
 DISCLOSURES
 REFERENCES
 
Cardiac fibroblasts (CF) express adenosine (ADO) receptors, and pharmacological evidence suggests the possible involvement of the A2 (A2a and A2b) receptor (A2aR and A2bR) subtypes in inhibiting cell functions involved in fibrosis. The main objective of this study was to define the contributions of A2a and/or A2b receptors in modulating ADO-induced decreases in CF functions. For this purpose, CF were either treated pharmacologically or had the A2aR or A2bR levels modified through the use of recombinant adenovirus or siRNA. The assessment of mRNA expression in adult rat CF yielded evidence for A1R, A2bR, A2aR, and A3R. Endogenously or exogenously enhanced ADO significantly inhibits CF proliferation, collagen, and protein synthesis. A2R and A2aR agonists, although capable of inhibiting CF protein and collagen synthesis, were unable to define the contributions derived from A2aR or A2bR. Overexpression of A2bR in CF yielded significant decreases in basal levels of collagen and protein synthesis and correlated with increases in cAMP levels. However, at higher doses of ADO receptor agonists, significant increases in protein and collagen synthesis were observed. CF with underexpression of A2bR yielded increases in protein and collagen synthesis. In contrast, A2aR underexpression did not modify ADO-induced decreases in CF protein or collagen synthesis. In conclusion, results derived from the molecular manipulation of receptor levels indicate that A2bR are critically involved in ADO-mediated inhibition of CF functions.

collagen deposition; extracellular matrix; remodeling

THE INCREASED INCIDENCE of congestive heart failure has stimulated therapeutic efforts to develop pharmacological strategies to prevent or treat the adverse processes of cardiac remodeling. Current therapies are mainly focused on blocking the actions of neurohormonal factors known to stimulate cardiac remodeling. However, the possibility of exploiting endogenously generated factors that are capable of inhibiting this process is only beginning to be assessed (25). An important component of the remodeling process observed with heart failure is the excess deposition of extracellular matrix (ECM) proteins (3). The main cell type responsible for the production of myocardial ECM proteins is the cardiac fibroblast (CF). Thus strategies intended to diminish the production of ECM proteins in the heart may specifically target surface receptors expressed by these cells.

The nucleoside adenosine (ADO) has been described as a retaliatory autocoid possessing homeostatic activities in the regulation of myocardial blood flow, catecholamine stimulation, and reduction of ischemic injury (30). Many of the effects that ADO exerts, such as the ability to suppress proinflammatory cytokine production and release, are consistent with the concept that ADO may inhibit the process of cardiac remodeling (22). There is indirect evidence that supports the hypothesis that enhanced endogenous ADO levels may attenuate the process of ventricular remodeling in heart failure. Loh et al. (24) observed that congestive heart failure patients that inherited a mutant AMP deaminase gene-1 experienced a significantly greater probability of survival without cardiac transplantation. The authors speculated that inheritance of this mutant allele might be advantageous to patients with congestive heart failure in that the reduction of AMP deaminage gene-1 activity leads to enhanced production of ADO in tissues. There is also evidence that enhanced ADO levels achieved by inhibition of cellular reuptake mechanisms may exert anti-fibrotic effects in models of postsurgery tissue adhesion (28).

Four main types of ADO receptors are known to exist: A1R, A2aR, A2bR, and A3R (23, 32). Fibroblasts are known to express these receptors, including the low-affinity A2bR (5). However, the expression profile of ADO receptors in CF has not been determined. ADO has recently been reported to be able to inhibit various CF functions. Data derived from the use of various selective and nonselective ADO receptor agonists and antagonists indicated that the effects were potentially mediated via A2bR (6, 9, 10). However, despite recent advances in the development of new agonists and antagonists, the use of pharmacological agents still poses significant limitations in our ability to discriminate which type of ADO receptors are involved. This is particularly true given the disparity in affinity for ADO agents that exists between two closely related Gs coupled receptor subtypes (A2a and A2b) and the fact that both of them appear to signal through common intracellular pathways (i.e., cAMP). Accordingly, in this study, we utilize molecular approaches to over- or underexpress A2aR or A2bR to ascertain for ADO receptor subtype involvement in the modulation of CF functions.


   MATERIALS AND METHODS
TOP
 ABSTRACT
 MATERIALS AND METHODS
RESULTS
 DISCUSSION
 GRANTS
 DISCLOSURES
 REFERENCES
 
CF isolation and culture. Primary adult rat CF cultures were generated from ventricular tissues of 6- to 8-wk-old male Sprague-Dawley rats (250–275 g), as previously described (33). Briefly, the rats were euthanized and the hearts were removed. The rats were treated according to the principles of the NIH "Guide for the Care and Use of Laboratory Animals" protocols.

Ventricular tissue was isolated, minced, and digested with the use of a solution containing collagenase (100 U/ml) and pancreatin (0.6 mg/ml). Isolated CF were seeded into 10-cm dishes containing growth media DMEM (GIBCO BRL), 10% FBS, and 1% penicillin-streptomycin-fungizone in a humidified atmosphere of 7% CO2. All studies were performed with cells at passage 2, and characterized as previously published (33).

CF treatment. CFs were initially grown to the desired confluency in growth media and rendered quiescent by serum starving for 24 h. For experiments involving the over- or underexpression of A2bR, 70% confluent CFs were exposed to recombinant A2bR adenovirus or small-interference RNAs (siRNAs). CF starvation was performed until adenovirus-infected or siRNA-transfected CF reached 100% confluency. Before treatment culture, the media were replaced with DMEM + 2.5% FBS. To test the effects of increasing endogenous or exogenous levels of ADO we used an adenosine kinase inhibitor (AKI), iodotubercidin (Itu), or an ADO analog, 2-chloroadenosine (Cl-Ad), respectively. Itu at the doses used has previously been shown to increase ADO levels in cultured CF (8). Micromolar 5'-(N-ethylcarboxamido) adenosine (NECA) was used as an ADO A2R agonist. The agonists 4-[N-ethy-(5'-carbamoyladenos-2yl)-aminoethyl]-phenylpropinoic acid (CGS-21680) or 4-{3-[6-amino-9-(5-ethylcarbamoyl-3,4-dihydroxy-tetrahydro-furan-2-yl)-9H-purin-2-yl]-prop-2ynyl}-cyclohexylmethyl ester (ATL-193) were used to selectively stimulate A2aR. All cell culture experiments were performed at least three times each in triplicate.

Construction of recombinant A2bR adenoviruses. Human A2bR (HA2bR) cDNA was a gift from Dr. D. Marquardt (University of California, San Diego). To construct the HA2bR-expressing adenovirus shuttle vector, the HA2bR cDNA was subcloned into the pACCMV.PLPASR(–) vector with the use of established methods (18). The resulting plasmid was confirmed with the use of restriction enzyme digestion and DNA sequencing. The HA2bR-expressing plasmid was then cotransfected into 293 cells with PJM17 using PolyFect transfection reagent (Qiagen; Valencia, CA). Plaques were isolated, propagated in 293 cells, and purified. The contamination of wild-type virus was ruled out by RT-PCR (35). Viral titers of purified stocks were then determined. To monitor transfection efficiency, green fluorescent protein (GFP)-expressing HA2bR recombinant adenoviruses were made according to an established protocol (19). A multiplicity of infection (MOI) of 200:1 yielded an 80% infection rate in CF without cytotoxicity (Fig. 1A). The HA2bR adenovirus was validated by RT-PCR and Western blot analysis (Fig. 1B). A control adenovirus was generated by recombination of reverse-oriented HA2bR cloned into the shuttle vector with PJM17. As assessed through RT-PCR, the overexpression of HA2bR in CF did not yield evidence of alterations in native rat A2aR or A2bR expression levels (data not shown).



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  		Fig. 1. Representative images of adenovirus-mediated overexpression of human A2b receptor (HA2bR) in cardiac fibroblasts (CF). A: CF infected with HA2bR-green fluorescent protein (GFP) virus under phase-contrast and fluorescence microscopy. A multiplicity of infection (MOI) ratio of 200:1 yielded ~80% CF infection without discernable cytotoxicity. B: mRNA (RT-PCR; top) and protein (Western blot; middle) levels obtained from CF-infected HA2bR viruses for 24 and 72 h, respectively. A pair of specific HA2bR recombinant primer was used to produce a ~1.3-kb exogenous HA2bR fragment. Endogenous and exogenous A2bR protein was detected by an anti-A2bR antibody that can cross react with human and rat A2bR. The densitometric scan values of HA2bR normalized to {beta}-actin bands from all experiments (n = 3) were converted to a bar graph (bottom). CF had a constitutive level of A2bR (lane 1). Control virus, which is reverse-oriented HA2bR adenovirus, did not affect A2bR expression in CF. CF infected with HA2bR adenoviruses at a MOI ratio of 200:1 demonstrated twofold A2bR overexpression.

 
Preparation and transfection of siRNA. Three pairs of rat A2aR or A2bR siRNA oligonucleotide templates were designed uniquely against rat A2aR or A2bR. The siRNA sequences targeting rat A2aR corresponded to the coding regions from 795, 1566, and 1913, and rat A2bR corresponded to the coding regions from 233, 539, and 718 plus the next 19 nucleotides as potential siRNAs. Sequences were blasted against genome databases to eliminate any sequences with homology to other genes. Selected target mRNA sequences for A2aR are the following: siRNA 795: 5'-AATTATCATGGTTTACTACAAC-3'; siRNA 1566: 5'-AAGGGAAGAGAAACCCAATGG-3'; and siRNA 1913: 5'-AAGTGGCACTTGGCTATTTCT-3'. The selected target mRNA sequences for A2bR are the following: siRNA 233: 5'-AACUACUUUCUGGUGUCCCUG-3'; siRNA539: 5'-AACAGUAAAGACCGUGCCACC-3'; siRNA718: 5'-AAUCUUCAUGGUGGCCUGCAA-3'; and control siRNA: 5'-AACGGCGACUACUAUGUGCUU-3'.

siRNA duplexes were synthesized with the use of a Silencer siRNA construction kit (Ambion; Austin, TX) and labeled with Cy3 to monitor transfection efficiency. The silencing capacity of the siRNAs was verified by RT-PCR and Western blot analysis. Of the three siRNAs designed, siRNA 1913 and siRNA539 were the most effective in silencing A2aR and A2bR, respectively, in CF (Fig. 2B). For siRNA transfection into CF, cells were grown in a 12-well plate to 70% confluency. CFs were divided into three groups for each A2aR or A2bR siRNA: transfection reagent only, transfected with control siRNA, A2aR siRNA1913, or A2bR siRNA539. siRNAs were introduced into CF by GenePORTER transfection reagent (Gene Therapy System; San Diego, CA). A final concentration of 0.17 µmol/l siRNA yielded 70% transfection efficiency with minimum cytotoxicity (Fig. 2A).



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  		Fig. 2. Representative images of small interference RNA (siRNA)-mediated silencing of A2bR and A2aR in CF. A: CF transfected with siRNA under phase-contrast and fluorescence microscopy. Cy3-labeled siRNAs (A, bottom) were transfected into the cytoplasm of CF with 70% transfection efficiency. In all experiments (n = 3 in triplicate), the transfection efficiency of 70% was consistent. B: mRNA (RT-PCR) and protein (Western blot analysis) levels obtained from CF transfected with A2bR (top) or A2aR (bottom) siRNAs for 72 h. As derived from densitometric scans, bar graphs summarized mRNA and protein expression levels after siRNA transfection (n = 3). As can be observed, rat A2aR siRNA1913 (lane 5) was the most effective siRNA to silence A2aR. mRNA and protein levels were reduced to 13% and 30%, respectively. For rat A2bR siRNA539 (lane 4) was the most effective siRNA to silence A2bR. mRNA and protein levels were reduced to 58% and 35%, respectively. GAPDH and actin were used for normalizing mRNA and protein levels, respectively.

 
RT-PCR. Total RNA was extracted with the use of a RNeasy Kit (Qiagen), followed by DNase digestion. One microgram of total RNA was then reverse transcribed into cDNA using RT-PCR Kit (Invitrogen; Carlsbad, CA). Four microliters of cDNA were used for PCR. PCR was performed with an initial step of denaturation at 94°C 3 min, 35 cycles of 30 s at 94°C, 30 s at 56°C, and 30 s or 60 s at 72°C (1 kb PCR product/min), followed by a final extension at 72°C for 10 min. To confirm CF overexpression of HA2bR after recombinant virus infection, a HA2bR-specific forward primer (5'-ctccatcttcagccttctgg-3') and a reverse primer from the multiple cloning site of SR(–) vector (5'-tgcaggtcgactctagaggat-3') were used to produce a ~1.3-kb fragment (Fig. 1B). To detect the silencing of rat A2aR by siRNA, a pair of specific rat A2aR primers (forward: 5'-ctcacgcagttccatcttccatctt-3' reverse: 5'-tccatctgcttcagctgtct-3') was used to amplify rat A2aR, resulting in a 371 bp fragment (Fig. 2B, top). To detect silencing of rat A2bR by siRNA, a pair of specific rat A2bR primers (forward: 5'-cttctgcacggactttcaca-3, reverse: 5'-ggtggcacggtctttactgt-3') was used to amplify rat A2bR, resulting in a 243 bp fragment (Fig. 2B, bottom). To demonstrate the presence of adenosine receptor subtypes on CF, we used four pair of specific rat primers for A1R, A2aR, A2bR, and A3R. A1R (5'-tacattggcatcgaggtgct-3', 5'-aggtgtggaagtaggtctgt-3'), A2aR (5'-ctcacgcagagttccatctt-3', 5'tccatctgcttcagctgtct-3'), A2bR (same as above), and A3R (5'-atatggctattcctgggcct-3', 5'accagaaacagggacttagc-3') primer pairs yield fragments of 205, 371, 243, and 326 bp respectively.

Western blot analysis. Three days after HA2bR recombinant virus infection or siRNA transfection, CF lysates were prepared in lysis buffer. Protein samples were separated and transferred onto membranes. Membranes were exposed to 1 µg/ml of an anti-A2aR or anti-A2bR polyclonal antibody (Alpha Diagnostic; San Antonio, TX) and then a secondary horseradish peroxidase-labeled antibody. Protein bands were visualized with the use of ECL plus (Amersham) (Fig. 2B).

[3H] assay incorporation. [3H]thymidine, [3H]leucine, and [3H]proline incorporation assays were used as a means to measure CF proliferation, protein and collagen synthesis respectively. Subconfluent (70%) CFs were used for the CF proliferation assay. Confluent CFs were used for protein and collagen synthesis assays. CFs were pulsed with [3H]thymidine, leucine, and proline. Experiments were terminated according to Dubey's protocol (6, 10). Radioactivity was counted in a liquid scintillation counter. CF proliferation was also assessed through cell counts.

cAMP assays. CF cAMP levels were determined utilizing a direct enzyme immunoassay kit according to manufacturer’s (Sigma-Aldrich; St. Louis, MO) instructions. CF were treated for a period of 10 min and cAMP precipitated with 0.1 N HCl. To prevent the breakdown of cAMP, the cells were pretreated with the phosphodiesterase inhibitor rolipram at 10 µmol/l for 15 min. To assess the functional coupling of A1R and A3R to Gi, pertussis toxin pretreatment at 100 ng/ml was provided to the cells before agonist treatment.

Statistical analysis. Statistical analysis was performed with Student's t-test or ANOVA. The results were considered statistically significant at P < 0.05.


   RESULTS
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 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
DISCUSSION
 GRANTS
 DISCLOSURES
 REFERENCES
 
ADO receptors in CF. RT-PCR was used to detect ADO A1R, A2aR, A2bR, and A3R mRNAs in CF. As shown in Fig. 3, ADO A1R, A2aR, A2bR, and A3R mRNAs were identified in CF. The approximate size of the bands was 205, 371, 243, and 326 base pairs for A1R, A2aR, A2bR, and A3R, respectively.



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  		Fig. 3. RT-PCR analysis of adenosine (ADO) receptor subtype mRNAs in CF. RT-PCR identified mRNAs for A1R, A2aR, A2bR, and A3R in CF. The size of the bands from CF was 205, 371, 243, and 326 bp for A1R, A2aR, A2bR, and A3R, respectively. Brain tissue was used for positive control.

 
Proliferation. Results indicate that Cl-Ad or AKI treatment inhibited cell proliferation in a concentration-dependent manner, as assessed through [3H]thymidine incorporation (Fig. 4A). In all cases, cells continued to proliferate, but the rates of proliferation were reduced with increases in either endogenous or exogenous adenosine levels. To determine the putative ADO receptor subtype-mediated CF proliferation, studies were performed using NECA, CGS-21680, or ATL-193. As one of the most potent A2bR agonists, NECA inhibited cell proliferation in micromolar concentrations (Fig. 4B). Treatment of CF with A2aR-specific agonists CGS-21680 and ATL-193 also resulted in inhibition of cell proliferation (Fig. 4C). However, compared with 10–5 mol/l NECA, which decreased the amount of incorporated [3H]thymidine to 43.2 ± 1.2% of control values, the inhibition of cell proliferation induced by either 10–8 mol/l CGS-21680 or ATL-193 was not as pronounced as that induced by NECA (Fig. 4C). Similar trends were observed on cell counts with the use of CGS-21680 and ATL-193 (Fig. 4F). NECA induced a decrease in cell number to 71.6 ± 2.6% of control. This decrease was more prominent than those induced by either CGS-21680 or ATL-193 (P < 0.05).



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  		Fig. 4. Effect of ADO agonists on CF proliferation. CF proliferation was assessed through [3H]thymidine incorporation assays (A–C) and cell counts (D–F). Data are means ± SE from three separate experiments each conducted in triplicate. NECA, 5'-(N-ethylcarboxamido) adenosine; AKI, adenosine kinase inhibitor. CGS, 4-[N-ethy-(5'-carbamoyladenos-2yl)-aminoethyl]-phenyl-propinoic acid (CGS-21680); ATL, 4-{3-[6-amino-9-(5-ethylcarbamoyl-3,4-dihydroxy-tetrahydro-furan-2-yl)-9H-purin-2-yl]-prop-2ynyl}-cyclohexylmethyl ester (ATL-193). *P < 0.05 vs. control (CO) values.

 
Protein synthesis. Results indicate that Cl-Ad and AKI significantly and dose dependently inhibited leucine incorporation (Fig. 5A). NECA also induced a dose-dependent decrease in protein synthesis (Fig. 5B). At 10–5 mol/l NECA decreased the amount of incorporated [3H]leucine to 62.9 ± 3.4% (P < 0.05). Treatment with either 10–8 mol/l CGS-21680 or 10–8 mol/l ATL-193 inhibited [3H]leucine incorporation to 71.4 ± 5.1% and 76.9 ± 5.2%, respectively (Fig. 5C).



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  		Fig. 5. Effect of adenosinergic agents on CF total protein and collagen synthesis. Protein (A–C) synthesis was assessed through [3H]-leucine incorporation assays and collagen (D–F). through [3H]-proline incorporation assays. Data were normalized to values derived from control untreated cells. Data are means ± SE from three separate experiments each conducted in triplicate. *P < 0.05 vs. control values.

 
The results of the effects of A2bR overexpression on CF protein synthesis are summarized in Fig. 6, A and B. Under basal (untreated) conditions, CF infected with HA2bR adenoviruses had a significant decrease in protein synthesis (P < 0.05 vs. control). However, when 10–4 mol/l Cl-Ad or 10–5 mol/l NECA were added to HA2bR overexpressing CF, increases in protein synthesis were observed (P < 0.05 vs. control). Control virus infection of CF did not affect protein synthesis compared with uninfected CF in the presence or absence of Cl-Ad or NECA.



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  		Fig. 6. Effects of 2-chloroadenosine (Cl-Ad) and NECA on HA2bR-overexpressing CF protein (A and B) and collagen (C and D) synthesis. Results were normalized to control uninfected values at the same dosage of Cl-Ad (A and C) or NECA (B and D) treatment. Data are means ± SE. Gray and black represent CF infected with control (reverse-oriented HA2bR) and HA2bR adenoviruses, respectively. *P < 0.05, ***P < 0.001, control vs. HA2bR-infected cells.

 
Silencing of A2bR led to increases in protein synthesis in the absence or presence of treatment with 10–6 mol/l Cl-Ad (P < 0.05) or 10–4 mol/l NECA (P < 0.001) (Fig. 7A). In contrast, silencing of A2aR did not modify the capacity of 10–6 mol/l Cl-Ad (P < 0.05) or 10–4 mol/l NECA to inhibit CF protein synthesis (Fig. 8A).



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  		Fig. 7. Effects of Cl-Ad and NECA on protein (A) and collagen (B) synthesis in A2bR siRNA-transfected CF. Values are means ± SE. Gray and black bars represent CF transfected with control siRNA and siRNA539, respectively. Control was untreated (neither Cl-Ad nor NECA was present). Values were normalized to those derived from untreated con siRNA-transfected cells. *P < 0.01, **P < 0.001, control siRNA vs. siRNA539.

 


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  		Fig. 8. Effects of Cl-Ad and NECA on protein (A) and collagen (B) synthesis in A2aR siRNA-transfected CF. Values are means ± SE. Gray and black bars represent CF transfected with control siRNA (con siRNA) and siRNA1913, respectively. Control was untreated (neither Cl-Ad nor NECA was present). Values were normalized to those derived from untreated control siRNA-transfected cells.

 
Collagen synthesis. As shown in Fig. 5D, Cl-Ad and AKI induced the inhibition of collagen synthesis in a concentration-dependent manner. NECA also induced a concentration-dependent inhibition of collagen synthesis (Fig. 5E). At 10–5 mol/l NECA suppressed collagen synthesis to 71.7 ± 0.8% (P < 0.05). Similar inhibition of collagen synthesis was observed with the treatment of CF with either CGS-21680 or ATL-193 at 10–8 mol/l (Fig. 5F).

CF infected with HA2bR adenoviruses had a significant decrease in collagen synthesis in the absence of agonist treatment. A significant decrease in CF collagen synthesis was also observed in cells treated with 10–6 mol/l Cl-Ad (P < 0.001) (Fig. 6C). However, when Cl-Ad or NECA, at 10–5 or 10–4 mol/l, respectively, was added to HA2bR overexpressing CF, a significant increase in collagen synthesis was observed (P < 0.001) (Fig. 6, C and D). Control virus infection of CF did not affect collagen synthesis compared with uninfected CF in the presence or absence of Cl-Ad or NECA.

Silencing of A2bR led to an increase in CF collagen synthesis in the presence or absence of agonists (Fig. 7B). In contrast, silencing of A2aR did not modify the capacity of 10–6 mol/l Cl-Ad (P < 0.05) or 10–4 mol/l NECA to inhibit CF collagen synthesis (Fig. 8B).

cAMP levels. The assessment of cAMP levels in CF was done in untreated cells and cells treated with 10–5 mol/l Cl-Ad or 10–4 mol/l NECA (Fig. 9). Results indicate that treatment of uninfected CF significantly increases cAMP levels. CF infected with a reverse-oriented HA2bR virus showed comparable results to those of uninfected cells. cAMP levels were also determined in sense HA2bR-overexpressing CF. Results indicate that overexpressing HA2bR in untreated CF yields enhanced levels of cAMP production (~10-fold vs. control or reverse-oriented control). The treatment of HA2bR overexpressing CF with Cl-Ad or NECA led to further increases in cAMP levels.



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  		Fig. 9. Determination of cAMP levels in either uninfected CF, reverse (R)-oriented HA2bR-infected CF, or HA2bR-infected CF sense (S) groups. Cells were either untreated or treated with Cl-Ad or NECA. Data are means ± SD. Significant differences vs. control were observed with agonist treatment within each group. Increases in cAMP levels in HA2bR-infected (S) CF were significantly different vs. corresponding controls or treated in either uninfected or R-infected cells.

 
A1R and A3R are known to mainly couple to Gi signaling (17) ADO agonist-induced increases in CF cAMP levels in the presence of pertussis toxin was used as a means to detect functional A1R and A3R. The results indicate that pertussis toxin pretreatment of CF did not yield greater levels of cAMP, thus indicating that CF do not express functional A1R and A3R (data not shown).


   DISCUSSION
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
GRANTS
 DISCLOSURES
 REFERENCES
 
Recent evidence indicates that ADO can act as an inhibitor of CF functions through its interaction with cell surface receptors. However, the role of ADO receptor subtypes, in particular A2bR, remains unclear. Through the use of molecular approaches (overexpressing or silencing A2aR and A2bR) we demonstrate important roles of A2bR in mediating CF functions. Furthermore, although CF express mRNA levels for all four types of ADO receptors, A1R and A3R do not appear functionally relevant as ascertained from the failure of these receptors to couple to Gi signaling.

The effects of ADO were explored in cultured CF by the exogenous addition of agents such as Cl-Ad or by endogenously enhancing its production through alterations in its metabolism. AKI treatment increases endogenous ADO levels by preventing the rephosphorylation of ADO into AMP (34). Results from the present study demonstrate that both exogenously (Cl-Ad) and endogenously (AKI) enhanced ADO levels can inhibit serum-induced CF proliferation in a dose-dependent manner. The magnitude of the decreases for Cl-Ad and AKI are comparable to those reported by Dubey et al. (6, 9, 10). A pharmacological approach was initially utilized to gain insight into the ADO receptor subtypes that may be involved in modulating CF functions. The ADO analog NECA remains one of the most potent A2bR agonists known (6, 9, 10, 12, 14, 15) with an EC50 of ~2 x 10–6 mol/l. In an attempt to ascertain the involvement of the A2bR we used NECA in the dose range of 10–6-10–4 mol/l. Treatment of CF with NECA decreased proliferation in a concentration-dependent manner. However, NECA can also activate other ADO receptor subtypes in this dose range. Thus the responses elicited by NECA at this dose range are suggestive of the involvement of A2bR but are not conclusive.

To further establish the relative contributions derived from A2aR and A2bR on cell proliferation we utilized agents known to preferentially act as selective A2aR agonists. CGS-21680 and ATL-193 in the nanomolar dose range in principle do not activate A2bR but are as potent as NECA in activating A2aR (1, 14). The agonist ATL-193 was selected because it has been reported to be 50 times more potent than CGS-21680 for A2aR binding (31). Both agents were used at concentrations of 10–8 mol/l. Results indicate that CGS-21680 and ATL-193-induced significant inhibitions (70–80% of control values) in CF thymidine incorporation suggesting a role of A2aR in CF proliferation. A similar trend was observed in cell count experiments. However, when these results are contrasted to those obtained with 10–5 mol/l concentrations of NECA (40% of control values), they suggest that A2bR may play a prominent role in inhibiting CF proliferation. These results differ from those reported by Dubey et al. (6, 10) that indicate that only micromoles per liter concentrations of CGS-21680 were capable of inhibiting CF proliferation. Altogether, our pharmacological results suggest that both A2aR and A2bR may participate in the modulation of CF proliferation.

Experiments performed to evaluate the effects of ADO on total protein synthesis also indicate that both exogenous and endogenous ADO is capable of inhibiting serum-stimulated CF leucine incorporation in a dose-dependent manner. Similar effects were observed with 10–5 mol/l NECA, which caused an inhibition of 62.9% of control values. Leucine incorporation experiments were also performed using the A2aR agonists CGS-21680 and ATL-193. We observed an inhibition (~75%) of control values with 10–8 mol/l CGS-21680 but no significant difference with ATL-193. Thus results derived from pharmacological treatment of the cells cannot elucidate the role of A2aR and A2bR in CF protein synthesis.

The incorporation of proline was used as a means to assess collagen synthesis. Significant dose-dependent decreases in proline incorporation were observed with Cl-Ad, AKI, and NECA. However, when the A2aR agonists were used, the magnitude of inhibition achieved was comparable to that generated by 10–6 mol/l NECA. These data suggest that A2R play important roles in regulating CF collagen synthesis.

As implied by the above discussion of results, the pharmacological characterization of A2bR-mediated cell functions is far from ideal. Selective A2bR agonists and antagonists are currently not readily available. A clearer understanding of the role that receptor subtypes may exercise in regulating cell functions can be gained by the use of molecular-based strategies. To elucidate how A2bR affects CF functions, we generated recombinant adenoviruses to overexpress A2bR. To control for any possible nonspecific effects of adenoviruses, control (reverse-oriented HA2bR) viruses were generated. The results indicate that infection of CF with control viruses did not affect leucine or proline synthesis compared with uninfected CF. The overexpression of A2bR in untreated CF yields significant decreases in basal collagen and protein synthesis. This inhibition likely represents the effects generated by the endogenous ADO because CFs produce significant amounts of ADO (20, 27). Indeed, the observation of enhanced cAMP levels in untreated, A2bR-overexpressing cells supports this concept. These results provide evidence for A2bR mediation of inhibitory actions on CF protein and collagen synthesis. Cl-Ad treatment at 10–6 mol/l decreased protein and collagen synthesis. However, when CF were treated with increasing doses of Cl-Ad or NECA, increases in collagen and protein synthesis were observed. These confounding responses could be due to abnormal receptor coupling (21) or secondary to the excessive production of cAMP (up to ~200-fold vs. controls). Alternatively, these responses may indicate that the regulation of protein and collagen synthesis by A2bR is inherently complex.

siRNA-based technology was chosen to silence rat A2aR or A2bR gene expression. We successfully transfected siRNA into CF yielding a substantial degree of A2bR mRNA and protein downregulation. CF with control siRNA transfection did not alter protein and collagen synthesis compared with CF with transfection reagent alone. The results indicate that the partial abolishment of A2bR expression yields the enhancement of basal CF protein and collagen synthesis. The enhancement of these CF functions was also present when cells were treated with 10–6 mol/l Cl-Ad or 10–4 mol/l NECA. The stimulatory effects were not observed in CF transfected with control siRNA. The fact that the partial absence of A2bR yields the stimulation of basal protein and collagen synthesis suggests that these receptors play key roles in the control of these functions. It also implies that an "imbalance" in ADO receptor subtype expression and activation (likely through endogenous ADO) alters basal cell functions. Altogether, given the absence of effects of A2aR silencing on ADO-mediated inhibition of CF protein or collagen synthesis, these results support the concept that CF protein and collagen synthesis are critically mediated with A2bR. It is worth noting that siRNA technology has proven to be minimally cytotoxic and highly specific for reducing the expression of targeted genes compared with antisense-based methodologies (29).

The signaling mechanisms responsible for A2bR-mediated inhibition of protein and/or collagen synthesis in CF are not fully known. A2bR are known to couple to Gs proteins and when activated can upregulate cAMP production (10). Increases in cAMP levels have been associated with the inhibition of cellular functions such as proliferation, DNA, protein and collagen synthesis (7, 8, 10, 11). Our results indicate that stimulation of CF with adenosine or NECA does indeed elevate cAMP levels within the cells, thus indicating the possibility of cAMP-mediated regulation of protein and/or collagen synthesis. However, as documented in other cell types, A2bR activation can also increase intracellular levels of calcium (2, 13, 16, 26) that can lead to activation of other signaling pathways. Thus the effects of the A2bR may be mediated by the simultaneous activation of different signal transduction pathways. There is also the possibility that A2bR activation may also inhibit protein and/or collagen synthesis through indirect actions such as the suppression of cytokine production (4). Thus much work remains to be done so as to understand how adenosine inhibits CF functions associated with fibrosis.

In conclusion, by utilizing molecular approaches, we provide evidence that A2bR may be critically involved in the inhibition of CF proliferation, protein, and collagen synthesis. The development of A2bR knockout animals should further clarify the role of A2bR in the modulation of these functions.


   GRANTS
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 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
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DISCLOSURES
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This study was supported by University of California Tobacco Related Disease Research Program Grant 3KT0709 and National Heart, Lung, and Blood Institute (NHLBI) Grants HL-03160 (to F. Villarreal) and R37HL-49434 (to W. Dillmann), as well as NHLBI Grant HL-07444 for training in Cardiovascular Physiology and Pharmacology at the University of California at San Diego.


   DISCLOSURES
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 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
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 DISCLOSURES
REFERENCES
 
The authors do not have any business, commercial, or other proprietary support, relationships, or interests that relate directly or indirectly to the subject of the study.


   ACKNOWLEDGMENTS
 
We thank Juan Alvergue for contributions to selected experiments.


   FOOTNOTES
 

Address for reprint requests and other correspondence: F. J. Villarreal, University of California at San Diego Medical Center, 200 W. Arbor Dr., San Diego, CA 92103-8412 (E-mail: fvillarr{at}ucsd.edu)

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.


   REFERENCES
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 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 GRANTS
 DISCLOSURES
 REFERENCES
 

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