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LETTERS TO THE EDITOR

Reply to "The antiapoptotic protein clusterin protects cardiomyocytes against ischemia-induced cell death"

Biomolecular Science Center, College of Medicine, University of Central Florida, Orlando, Florida

REPLY: We thank Krijnen and Niessen (1) for their interest in our study (3) and welcome the opportunity to respond to their comments. First, we do not claim that clusterin is the only major protein that acts as an antiapoptotic agent and protects against H₂O₂-induced apoptosis in H9c2 cells. We have shown that four released factors from embryonic stem cells, including clusterin, display antiapoptotic activity (3). In fact, we consider that TIMP-1 is the major antiapoptotic protein present in embryonic stem cells-conditioned medium. Second, we missed their article (2) reporting antiapoptotic properties of clusterin in the ischemic cell culture model of H9c2 cells (2); however, our cell culture model of H₂O₂-induced apoptosis is different. Finally, we suggest that clusterin may not be a major antiapoptotic protein in our cell culture model. However, future studies are needed to fully understand this protein.

FOOTNOTES

Address for reprint requests and other correspondence: D. K. Singla, Biomolecular Science Ctr., Univ. of Central Florida, Orlando, FL 32817 (e-mail: dsingla{at}mail.ucf.edu)

REFERENCES

1. Krijnen P, Niessen H. The antiapoptotic protein clusterin protects cardiomyocytes against ischemia-induced cell death. Am J Physiol Heart Circ Physiol doi:10.1152/ajpheart.00964.2007.
2. Krijnen PA, Cillessen SA, Manoe R, Muller A, Visser CA, Meijer CJ, Musters RJ, Hack CE, Aarden LA, Niessen HW. Clusterin: a protective mediator for ischemic cardiomyocytes? Am J Physiol Heart Circ Physiol 289: H2193–H2202, 2005.[Abstract/Free Full Text]
3. Singla DK, McDonald DE. Factors released from embryonic stem cells inhibit apoptosis of H9c2 cells. Am J Physiol Heart Circ Physiol 293: H1590–H1595, 2007.[Abstract/Free Full Text]

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