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Am J Physiol Heart Circ Physiol (August 28, 2003). doi:10.1152/ajpheart.00002.2003
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Submitted on January 9, 2003
Accepted on August 21, 2003

Stretch-Induced Calcium Sensitization of Rat Lymphatic Smooth Muscle

Yuichi Shirasawa1 and Joseph N. Benoit1*

1 Department of Pharmacology, Physiology and Therapeutics, University of North Dakota, Grand Forks, ND, USA

* To whom correspondence should be addressed. E-mail: j_benoit{at}mail.und.nodak.edu.

The relationships between smooth muscle calcium and isometric tension generation to spontaneous lymphatic pump activity and its modulation by stretch equivalent to 0 to ~6 cmH2O were investigated. Excised preparations of rat thoracic duct were mounted on a wire myograph and loaded with the calcium sensitive fluorochrome, indo-1. Calcium dependent fluorescence and isometric force were simultaneously recorded. The thoracic duct segments developed spontaneous rhythmic contractile activity. Each contraction was preceded by an increase in intracellular calcium. When the vessels were normalized and stabilized at a preload equal to 3 cmH2O, the peak generation in tension occurred 0.70 ± 0.11 sec after that of calcium. Incremental stretch enhanced frequency of the phasic activity and amplitude of isometric force generation but neither the basal calcium level nor the amplitude of the calcium transient. These findings suggest that stretch enhances lymphatic pump activity by increasing the pacemaker activity and the calcium sensitivity of the contractile apparatus.




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