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Articles in PresS, published online ahead of print February 28, 2002
Am J Physiol Heart Circ Physiol, 10.1152/ajpheart.00042.2002
Submitted on January 20, 2002
Accepted on February 26, 2002
1 Biochemistry, University of Alberta, Edmonton, Alberta, Canada
* To whom correspondence should be addressed. E-mail: lfliegel{at}gpu.srv.ualberta.ca.
We examined the hypothesis that Na+/H+ exchanger expression is regulated during fetal and neonatal development and differentiation. To examine transcriptional regulation of the NHE1 isoform of the Na+/H+ exchanger, transgenic mice were created that contained the mouse NHE1 promoter driving expression of green fluorescent protein. The level of NHE1 transcription varied between tissues and with the stage of embryonic development. The highest expression was in the heart and liver of 12 to 15-day old mice and this declined with age. To examine Na+/H+ exchanger protein levels, we immunoblotted mouse tissues from 18-day old embryos, neonates and adults. Protein levels increased after embryonic day 18 and peaked at 14 days of age in the heart, lung, liver, kidney and brain. The greatest rise in NHE1 protein expression occurred in the heart while the smallest increase was in the brain. The results suggest that Na+/H+ exchanger transcription and protein levels are controlled in a tissue-specific and time-dependent manner during development.
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