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Am J Physiol Heart Circ Physiol (May 9, 2002). doi:10.1152/ajpheart.00096.2002
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Articles in PresS, published online ahead of print May 7, 2002
Am J Physiol Heart Circ Physiol, 10.1152/ajpheart.00096.2002
Submitted on February 6, 2002
Accepted on April 29, 2002

Initiation and propagation of ectopic waves: insights from an in vitro model of ischemia/reperfusion injury

Ara Arutunyan1, Luther M. Swift1, and Narine Sarvazyan1*

1 Physiology, Texas Tech University Health Sciences Center, Lubbock, TX, USA

* To whom correspondence should be addressed. E-mail: Narine.Sarvazyan{at}ttmc.ttuhsc.edu.

The objective of the present study was to directly visualize ectopic activity associated with ischemia/reperfusion and its progression to arrhythmia. To accomplish this goal, we employed a two-dimensional network of neonatal rat cardiomyocytes and a recently developed model of localized ischemia/reperfusion. Washout of the ischemia-like solution resulted in tachyarrhythmic episodes lasting 15-200s. These episodes were preceded by the appearance of multiple ectopic sources and propagation of ectopic activity along the border of the former ischemic zone. The ectopic sources exhibited a slow rise in diastolic calcium, which disappeared upon return to the original pacing pattern. Border zone propagation of ectopic activity was followed by its escape into the surrounding control network, generating arrhythmias. Together, these observations suggest that upon reperfusion a distinct layer, which consists of ectopically active, poorly coupled cells, is formed transiently over an injured area. Despite being neighbored by a conductive and excitable tissue, this transient functional layer is capable of sustaining autonomous waves and serving as a special conductive medium through which ectopic activity can propagate before spreading into the surrounding healthy tissue.




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