Regulation of cytosolic calcium and myofilament calcium sensitivity vary considerably with postnatal age in cerebral arteries. Because these mechanisms also govern myogenic tone, the present study used graded stretch to examine the hypothesis that myogenic tone is less dependent on calcium influx and more dependent on myofilament calcium sensitization in term fetal compared to adult cerebral arteries. Term fetal and adult posterior communicating cerebral arteries exhibited similar myogenic responses with peak tensions averaging 24% and 26% of Kmax at optimum stretch ratios (working diameter / unstressed diameter) of 2.19 and 2.23, respectively. Graded stretch increased cytosolic [Ca⁺⁺] at stretch ratios >2.0 in adult arteries, but increased [Ca⁺⁺] only at stretch ratios >2.3 in fetal arteries. In permeabilized arteries, myogenic tone peaked at a stretch ratio of 2.1 in both fetal and adult arteries. The fetal %Kmax values at peak myogenic tone were not significantly different at either pCa 7.0 (23%) or pCa 5.5 (25%), but were significantly less at pCa 8.0 (8.4 ± 2.3%). Conversely, adult %Kmax values at peak myogenic tone were significantly less at both pCa 8.0 (10.4 ± 1.8%) and pCa 7.0 (16%) than at pCa 5.5 (27%). The maximal extents of stretch-induced increases in MLC phosphorylation in intact fetal (20%) and adult (17%) arteries were similar. The data demonstrate that the cerebrovascular myogenic response is highly conserved during postnatal maturation but is mediated differently in fetal and adult cerebral arteries.