AJP - Heart Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol (June 3, 2005). doi:10.1152/ajpheart.00153.2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
289/5/H1976    most recent
00153.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smith, D. F
Right arrow Articles by Huo, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smith, D. F
Right arrow Articles by Huo, Y.
Submitted on February 15, 2005
Accepted on June 1, 2005

GRO Family Chemokines are Specialized for Monocyte Arrest from Flow

David F Smith1*, Elena Galkina2, Klaus Ley3, and Yuqing Huo2

1 Department of Molecular Physiology and Biophysics, University of Virginia, Charlottesville, VA, USA
2 Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA
3 Department of Molecular Physiology and Biophysics, University of Virginia, Charlottesville, VA, USA; Cardiovascular Research Center, Univeristy of Virginia, Charlottesville, VA, USA; Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA

* To whom correspondence should be addressed. E-mail: dfs4j{at}virginia.edu.

Chemokines participate in various processes of monocyte recruitment including monocyte arrest and migration. Our group and others have demonstrated that growth related oncogene (GRO)-{alpha} (CXCL1) could support monocyte arrest in models of inflammation. Here we employed a parallel plate flow chamber and transwell reconstitution assay to test whether GRO family chemokines were sufficient for Mono Mac 6, a human monocytic cell line, and isolated human monocyte recruitment. Our study shows that: (a) GRO-{alpha}, {beta} (CXCL2) and {gamma} (CXCL3) all act as arrest chemokines for monocyte adhesion on vascular cell adhesion molecule (VCAM)-1 under flow in the presence of P-selectin. (b) CXCR2 is the functional receptor for GRO family chemokines in monocyte arrest. However, CXCR2 is not an arrest chemokine receptor in general, since ENA-78 failed to arrest monocytes. (c) GRO-{alpha}, {beta} and {gamma} all fail to raise intracellular free calcium or mediate monocyte chemotaxis. (d) Signaling through G{alpha}i protein, phosphoinositide 3-kinase (PI3K), and actin polymerization, but not calcium mobilization or the mitogen-activated kinases p-38 and MEK, are necessary for GRO-{alpha}-mediated Mono Mac 6 cell arrest under flow. We conclude that the GRO family chemokines are specialized monocyte arrest chemokines. Their role in monocyte recruitment in inflammation can be inhibited by blocking CXCR2 function or downstream signaling events.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
A. Zarbock, H. Muller, Y. Kuwano, and K. Ley
PSGL-1-dependent myeloid leukocyte activation
J. Leukoc. Biol., November 1, 2009; 86(5): 1119 - 1124.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. R. Wilson, K. P. O'Dea, D. Zhang, A. D. Shearman, N. van Rooijen, and M. Takata
Role of Lung-marginated Monocytes in an In Vivo Mouse Model of Ventilator-induced Lung Injury
Am. J. Respir. Crit. Care Med., May 15, 2009; 179(10): 914 - 922.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
C. A. Gleissner, P. von Hundelshausen, and K. Ley
Platelet Chemokines in Vascular Disease
Arterioscler Thromb Vasc Biol, November 1, 2008; 28(11): 1920 - 1927.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. E. Cardona, M. Li, L. Liu, C. Savarin, and R. M. Ransohoff
Chemokines in and out of the central nervous system: much more than chemotaxis and inflammation
J. Leukoc. Biol., September 1, 2008; 84(3): 587 - 594.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
M. J. Zilliox, W. J. Moss, and D. E. Griffin
Gene Expression Changes in Peripheral Blood Mononuclear Cells during Measles Virus Infection
Clin. Vaccine Immunol., July 1, 2007; 14(7): 918 - 923.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Wolnicka-Glubisz, J. Damsker, S. Constant, S. Corn, E. De Fabo, and F. Noonan
Deficient inflammatory response to UV radiation in neonatal mice
J. Leukoc. Biol., June 1, 2007; 81(6): 1352 - 1361.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. Hristov, A. Zernecke, K. Bidzhekov, E. A. Liehn, E. Shagdarsuren, A. Ludwig, and C. Weber
Importance of CXC Chemokine Receptor 2 in the Homing of Human Peripheral Blood Endothelial Progenitor Cells to Sites of Arterial Injury
Circ. Res., March 2, 2007; 100(4): 590 - 597.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
D. F. Smith, T. L. Deem, A. C. Bruce, J. Reutershan, D. Wu, and K. Ley
Leukocyte phosphoinositide-3 kinase {gamma} is required for chemokine-induced, sustained adhesion under flow in vivo
J. Leukoc. Biol., December 1, 2006; 80(6): 1491 - 1499.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
P. S. Gargalovic, N. M. Gharavi, M. J. Clark, J. Pagnon, W.-P. Yang, A. He, A. Truong, T. Baruch-Oren, J. A. Berliner, T. G. Kirchgessner, et al.
The Unfolded Protein Response Is an Important Regulator of Inflammatory Genes in Endothelial Cells
Arterioscler Thromb Vasc Biol, November 1, 2006; 26(11): 2490 - 2496.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
H. L. Rittner, S. A. Mousa, D. Labuz, K. Beschmann, M. Schafer, C. Stein, and A. Brack
Selective local PMN recruitment by CXCL1 or CXCL2/3 injection does not cause inflammatory pain
J. Leukoc. Biol., May 1, 2006; 79(5): 1022 - 1032.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
D. Bernhard, A. Rossmann, B. Henderson, M. Kind, A. Seubert, and G. Wick
Increased Serum Cadmium and Strontium Levels in Young Smokers: Effects on Arterial Endothelial Cell Gene Transcription
Arterioscler Thromb Vasc Biol, April 1, 2006; 26(4): 833 - 838.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.