| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Human Physiology, School of Medicine, University of California, Davis, CA, USA
* To whom correspondence should be addressed. E-mail: rhadamson{at}ucdavis.edu.
Thrombin is widely used to stimulate a variety of responses in cultured endothelial cell monolayers as a model of acute vascular endothelial reponse to inflammatory mediators. However, preliminary results indicated that rat mesenteric venules did not respond acutely to thrombin. We tested the hypothesis that rat venules would respond to thrombin 24 hr after prior injury by microperfusion. Vessel responsiveness was measured as hydraulic conductivity (Lp). When venules were exposed to rat thrombin (10 U/ml) within 2 hr of initial perfusion with vehicle control, there was no increase in Lp of any vessel from a mean baseline of 1.2 ± 0.2 x 10-7 cm/(s cmH2O). In contrast, when perfused with thrombin at 25-27 hours after initial perfusion, every venule responded to thrombin with a transient increase in Lp. The mean peak Lp on Day 2 in response to thrombin was 24 ± 4.2 x 10-7 cm/(s cmH2O). Our results suggest that prior endothelial injury modifies the endothelial cell phenotype and alters the response of endothelial cells to thrombin after 24 hours. Phenotypic plasticity of endothelial cells may play a key role in the regulation of permeability of some endothelial cells in culture and in intact venules, where localized leaky sites may form where there had been a previous inflammatory response.
This article has been cited by other articles:
|
|
 |
|
  W.-M. Heupel, A. Efthymiadis, N. Schlegel, T. Muller, Y. Baumer, W. Baumgartner, D. Drenckhahn, and J. Waschke Endothelial barrier stabilization by a cyclic tandem peptide targeting VE-cadherin transinteraction in vitro and in vivo J. Cell Sci., May 15, 2009; 122(10): 1616 - 1625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-H. Kim, F.-R. E. Curry, and S. I. Simon Dynamics of neutrophil extravasation and vascular permeability are uncoupled during aseptic cutaneous wounding Am J Physiol Cell Physiol, April 1, 2009; 296(4): C848 - C856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. P. van Nieuw Amerongen, R. J. P. Musters, E. C. Eringa, P. Sipkema, and V. W. M. van Hinsbergh Thrombin-induced endothelial barrier disruption in intact microvessels: role of RhoA/Rho kinase-myosin phosphatase axis Am J Physiol Cell Physiol, May 1, 2008; 294(5): C1234 - C1241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-R. E. Curry Permeability measurements in an individually perfused capillary: the 'squid axon' of the microcirculation Exp Physiol, April 1, 2008; 93(4): 444 - 446. [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Adamson, J. C. Ly, R. K. Sarai, J. F. Lenz, A. Altangerel, D. Drenckhahn, and F. E. Curry Epac/Rap1 pathway regulates microvascular hyperpermeability induced by PAF in rat mesentery Am J Physiol Heart Circ Physiol, March 1, 2008; 294(3): H1188 - H1196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Troyanovsky, D. F. Alvarez, J. A. King, and K. L. Schaphorst Thrombin enhances the barrier function of rat microvascular endothelium in a PAR-1-dependent manner Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L266 - L275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Waschke, D. Drenckhahn, R. H. Adamson, H. Barth, and F. E. Curry cAMP protects endothelial barrier functions by preventing Rac-1 inhibition Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2427 - H2433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. M. Chandrasekharan, L. Yang, A. Walters, P. Howe, and P. E. DiCorleto Role of CL-100, a Dual Specificity Phosphatase, in Thrombin-induced Endothelial Cell Activation J. Biol. Chem., November 5, 2004; 279(45): 46678 - 46685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Adamson, J. F. Lenz, X. Zhang, G. N. Adamson, S. Weinbaum, and F. E. Curry Oncotic pressures opposing filtration across non-fenestrated rat microvessels J. Physiol., June 15, 2004; 557(3): 889 - 907. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
