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1 Department of Physiology, University of Wisconsin-Madison, Madison, WI, USA
* To whom correspondence should be addressed. E-mail: campbell{at}physiology.wisc.edu.
SLControl is a computerized data acquisition and analysis system which was developed in our laboratory to help perform mechanical experiments using striated muscle preparations. It consists of a computer program (Windows 2000 or later) and a commercially available data acquisition board (16-bit resolution, DAP5216a, Microstar Laboratories, Bellevue, WA). Signals (from the user's existing equipment) representing force, fiber length (FL) and (if desired) sarcomere length (SL) are connected to the system through standard BNC cables and saved to data files for later analysis. Output signals from the board control FL and trigger additional equipment e.g. flash-lamps. Windows dialogs drive a number of different experimental protocols including slack tests and rate of tension recovery measurements. Precise measurements of muscle stiffness and force-velocity/power characteristics can also be accomplished using SL and tension control respectively. In these situations, the FL command signal is updated in real time (at rates
2.5 kHz) in response to changes in the measured SL or force signals. Data files can be exported as raw text or analyzed within SLControl using built-in tools for cursor analysis, digital filtering, curve-fitting etc. The software is available for free download at http://www.slcontrol.com.
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