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Am J Physiol Heart Circ Physiol (January 2, 2003). doi:10.1152/ajpheart.00323.2002
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Submitted on May 2, 2002
Accepted on December 16, 2002

Coronary flow-induced inotropism is modulated by binding of dextrans to the endothelial luminal surface

Carmen Gonzalez-Castillo1, Rafael Rubio1*, and Tania Zenteno-Savin2

1 Deptamento de Fisiologia y Farmacologia, Univ. Aut. de San Luis Potosi, San Luis Potosi, S.L.P, Mexico
2 Centro de Investigaciones Biologicas del Noroeste, Mexico

* To whom correspondence should be addressed. E-mail: rrubio{at}uaslp.mx.

In isolated perfused guinea pig heart, coronary flow causes a positive inotropic effect (+CFIE) that could be altered by dextrans (Dx) in the coronary perfusion solution. To test this possibility Dx of 20, 40, 70 and 500 kD were infused and found to modulate +CFIE, however, when Dx infusion was terminated the effect persisted i. e. was irreversible/non-washable suggesting that Dx may bind to luminal endothelial lectinic structures. This hypothesis was tested a) when Dx (with fluorescent traces; D*) bound to the vessel wall was hydrolyzed by dextranase infusion and washout of D* fragments completely reverted the +CFIE, and b) bound D* to be displaced by free Dx required concentrations 50-100 times that used during binding. In addition, dose-response curves for Dx on +CFIE show that the higher the dextran molecular weight the lesser the concentration required to have an effect. Because a large dextran molecule have a greater number polymeric glucose branches it can bind to a larger number of endothelial lectinic sites requiring a lower concentration to affect +CFIE. Our results suggest that luminal endothelial lectinic structures are part of the flow-sensing assembly.




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