| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print June 20, 2002
Am J Physiol Heart Circ Physiol, 10.1152/ajpheart.00335.2002
Submitted on April 15, 2002
Accepted on June 17, 2002
1 Department of Pathology, Duke University Medical Center, Durham, NC, USA
2 Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA
3 Department of Pharmacology, Medical College of Wisconsin, Milwaukee, WI, USA
* To whom correspondence should be addressed. E-mail: cross017{at}mc.duke.edu.
To determine whether A3-adenosine receptor (A3AR) signaling modulates myocardial function, energetics and cardioprotection, hearts from wild-type and A3AR-overexpressor mice were subjected to 20 min ischemia/40 min reperfusion while 31P NMR spectra were acquired. Basal heart rate and developed pressure (LVDP) were lower in A3AR-overexpressor hearts than wild-type. Ischemic ATP depletion was delayed and post-ischemic recoveries of contractile function, ATP and PCr were greater in A3AR-hearts. To determine the role of depressed heart rate and confirm A3AR-specific signaling, hearts were paced at 480 bpm ± 60 nmol/L MRS-1220 (A3AR-specific inhibitor) then subjected to ischemia/reperfusion. LVDP was similar in paced-A3AR vs. paced wild-type hearts. Differences in ischemic ATP depletion and post-ischemic contractile and energetic dysfunction remained in paced A3AR-overexpressor hearts vs. paced wild-type but were abolished by MRS-1220. In summary, A3AR-overexpression decreased basal heart rate and contractility, preserved ischemic ATP and decreased post-ischemic dysfunction. Pacing abolished the decreased contractility but not the ATP preservation or cardioprotection. Therefore, A3AR-overexpression results in cardioprotection via a specific A3AR-effect, possibly involving preservation of ATP during ischemia.
This article has been cited by other articles:
|
|
 |
|
  R. R. Morrison, B. Teng, P. J. Oldenburg, L. C. Katwa, J. B. Schnermann, and S. J. Mustafa Effects of targeted deletion of A1 adenosine receptors on postischemic cardiac function and expression of adenosine receptor subtypes Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1875 - H1882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ruiz-Meana, D. Garcia-Dorado, E. Miro-Casas, A. Abellan, and J. Soler-Soler Mitochondrial Ca2+ uptake during simulated ischemia does not affect permeability transition pore opening upon simulated reperfusion Cardiovasc Res, September 1, 2006; 71(4): 715 - 724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Fabritz, P. Kirchhof, L. Fortmuller, J. A Auchampach, H. A Baba, G. Breithardt, J. Neumann, P. Boknik, and W. Schmitz Gene dose-dependent atrial arrhythmias, heart block, and brady-cardiomyopathy in mice overexpressing A3 adenosine receptors Cardiovasc Res, June 1, 2004; 62(3): 500 - 508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Headrick, B. Hack, and K. J. Ashton Acute adenosinergic cardioprotection in ischemic-reperfused hearts Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H1797 - H1818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen and R. J. Bache Adenosine: A Modulator of the Cardiac Response to Stress Circ. Res., October 17, 2003; 93(8): 691 - 693. [Full Text] [PDF]
|
|
|
|
|
|
J. N. Peart and G. J. Gross Adenosine and opioid receptor-mediated cardioprotection in the rat: evidence for cross-talk between receptors Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H81 - H89. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
