| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print June 20, 2002
Am J Physiol Heart Circ Physiol, 10.1152/ajpheart.00347.2002
Submitted on April 29, 2002
Accepted on June 14, 2002
1 School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom
* To whom correspondence should be addressed. E-mail: bmsfpb{at}bms.leeds.ac.uk.
Kawai et al. (Am. J. Physiol 277: H603-H609, 1999) developed a technique to detubulate rat ventricular myocytes using formamide, and showed that detubulation results in a decrease in cell capacitance, calcium current density and calcium transient amplitude. We have investigated the mechanism of this detubulation and possible direct effects of formamide. Staining ventricular cells with di-8-ANEPPS showed that the t-tubules remain inside the cell after detubulation; trapping of FITC-labelled dextran within the t-tubules showed that detubulation occurs during formamide washout and that the t-tubules appear to reseal within the cell. Detubulation had no effect on the microtubule network, but resulted in loss of synchronous Ca2+ release on electrical stimulation. In contrast, formamide treatment of atrial cells did not significantly change cell capacitance, calcium current amplitude, action potential configuration, the calcium transient or the response of the calcium transient to isoprenaline. We conclude that formamide washout induces detubulation of single rat ventricular myocytes, leaving the t-tubules within the cell, but without direct effects on cell proteins that might alter cell function.
This article has been cited by other articles:
|
|
 |
|
  C. H. Orchard, M. Pasek, and F. Brette The role of mammalian cardiac t-tubules in excitation-contraction coupling: experimental and computational approaches Exp Physiol, May 1, 2009; 94(5): 509 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Abstracts Heart, February 1, 2009; 95(4): e1 - e1. [Full Text] [PDF]
|
|
|
|
 |
|
 F. Swift, J. A. K. Birkeland, N. Tovsrud, U. H. Enger, J. M. Aronsen, W. E. Louch, I. Sjaastad, and O. M. Sejersted Altered Na+/Ca2+-exchanger activity due to downregulation of Na+/K+-ATPase {alpha}2-isoform in heart failure Cardiovasc Res, April 1, 2008; 78(1): 71 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. P.M. M. Lauritzen, T. Ploug, H. Ai, M. Donsmark, C. Prats, and H. Galbo Denervation and High-Fat Diet Reduce Insulin Signaling in T-Tubules in Skeletal Muscle of Living Mice Diabetes, January 1, 2008; 57(1): 13 - 23. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Despa and D. M. Bers Functional analysis of Na+/K+-ATPase isoform distribution in rat ventricular myocytes Am J Physiol Cell Physiol, July 1, 2007; 293(1): C321 - C327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Brette and C. Orchard Resurgence of Cardiac T-Tubule Research Physiology, June 1, 2007; 22(3): 167 - 173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Berry, S. Despa, W. Fuller, D. M. Bers, and M. J. Shattock Differential distribution and regulation of mouse cardiac Na+/K+-ATPase {alpha}1 and {alpha}2 subunits in T-tubule and surface sarcolemmal membranes Cardiovasc Res, January 1, 2007; 73(1): 92 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Gorelik, L. Q. Yang, Y. Zhang, M. Lab, Y. Korchev, and S. E. Harding A novel Z-groove index characterizing myocardial surface structure Cardiovasc Res, December 1, 2006; 72(3): 422 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. Shiels and E. White Temporal and spatial properties of cellular Ca2+ flux in trout ventricular myocytes Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1756 - R1766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Fowler, J. R. Naz, M. D. Graham, G. Bru-Mercier, S. M. Harrison, and C. H. Orchard Decreased Ca2+ extrusion via Na+/Ca2+ exchange in epicardial left ventricular myocytes during compensated hypertrophy Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2431 - H2438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R Fowler, R. S Dobson, C. H Orchard, and S. M Harrison Functional consequences of detubulation of isolated rat ventricular myocytes Cardiovasc Res, June 1, 2004; 62(3): 529 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. G Perez, M. C Villa-Abrille, E. A Aiello, R. A Dulce, H. E Cingolani, and M. C Camilion de Hurtado A low dose of angiotensin II increases inotropism through activation of reverse Na+/Ca2+ exchange by endothelin release Cardiovasc Res, December 1, 2003; 60(3): 589 - 597. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
