AJP - Heart Calcium Transients and Cell-Sarcomere
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Am J Physiol Heart Circ Physiol (June 20, 2002). doi:10.1152/ajpheart.00362.2002
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Articles in PresS, published online ahead of print June 20, 2002
Am J Physiol Heart Circ Physiol, 10.1152/ajpheart.00362.2002
Submitted on April 24, 2002
Accepted on June 14, 2002

An Implantable Bolus Infusion Pump For Use In Freely-Moving, Non-tethered Rats

Daniel P. Holschneider1*, Jean-Michel I. Maarek2, Juji Harimoto2, Jun Yang3, and Oscar U. Scremin4

1 Psychiatry and the Behavioral Sciences, University of Southern California, Keck School of Medicine, Los Angeles, CA, USA; Neurology, University of Southern California, Keck School of Medicine, Los Angeles, CA, USA; Research, Greater Los Angeles VA Healthcare System, Los Angeles, CA, USA
2 Biomedical Engineering, University of Southern California, School of Engineering, Los Angeles, CA, USA
3 Psychiatry and the Behavioral Sciences, University of Southern California, Keck School of Medicine, Los Angeles, CA, USA
4 Research, Greater Los Angeles VA Healthcare System, Los Angeles, CA, USA; Physiology, University of California at Los Angeles, School of Medicine, Los Angeles, CA, USA

* To whom correspondence should be addressed. E-mail: holschne{at}hsc.usc.edu.

One of the current constraints on functional neuroimaging in animals is that in order to avoid movement artifacts during data acquisition, subjects need to be immobilized, sedated or anesthetized. Such measures limit the behaviors that can be examined, and introduce the additional variables of stress or anesthetic agents that may confound meaningful interpretation. This study provides a description of the design and characteristics of a self-contained, implantable microbolus infusion pump (MIP) that allows triggering of a bolus injection at a distance in conscious, behaving rats not restrained or tethered. The MIP is externally triggered by a pulse of infrared light and allows in-vivo bolus drug delivery. We describe application of this technology to the intravenous bolus delivery of 14C-iodo-antipyrine in a freely moving animal, followed immediately by lethal injection, rapid removal of the brain and analysis of regional cerebral blood flow tissue radioactivity using autoradiography. The ability to investigate changes in brain activation in nonrestrained animals makes the MIP a powerful tool for evaluation of complex behaviors.






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