AJP - Heart Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol (January 13, 2006). doi:10.1152/ajpheart.00412.2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
290/6/H2453    most recent
00412.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Traverse, J. H
Right arrow Articles by Bache, R. J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Traverse, J. H
Right arrow Articles by Bache, R. J
Submitted on April 25, 2005
Accepted on January 9, 2006

Measurement of Myocardial Free Radical Production During Exercise Using EPR Spectrscopy

Jay H Traverse1*, Yuri E Nesmelov2, Melanie Crampton3, Paul Lindstrom3, David D Thomas2, and Robert J Bache3

1 Medicine, University of Minnesota, Minneapolis, MN, USA; Biochemistry, University of Minnesota, Minneapolis, MN, USA
2 Cardiology, Minneapolis Heart Institute at Abbott Northwestern Hospital, Minneapolis, MN, USA
3 Medicine, University of Minnesota, Minneapolis, MN, USA

* To whom correspondence should be addressed. E-mail: trave004{at}umn.edu.

Exercise is associated with an increase in oxygen flux through the mitochondrial electron transport chain that has recently been demonstrated to increase the production of reactive oxygen species (ROS) in skeletal muscle. This study examined whether exercise also causes free radical production in the heart. We measured ROS production in 7 chronically instrumented dogs during rest and treadmill exercise (6.4 Km/H, 10°grade; heart rate 204 ± 3 beats/min) using EPR spectroscopy in conjunction with the spin trap {alpha}-phenyl - tert -butylnitrone (PBN) (0.14 mol/L) in blood collected from the aorta and coronary sinus (CS). To improve signal detection, the free radical adducts were deoxygenated over a nitrogen stream for 15 minutes and extracted with toluene. The hyperfine splitting constants of the radicals were {alpha}N = 13.7 G and {alpha}H = 1.0 G, consistent with an alkoxyl or carbon centered radical. Resting aortic and CS PBN adduct concentrations were 6.7 and 6.3 (A.U x 108 ) (p=NS). Both aortic and CS adduct concentrations increased during exercise, but there was no significant difference between the aortic and CS concentrations. Thus, in contrast to skeletal muscle, submaximal treadmill exercise did not result in detectable free radical production by the heart.




This article has been cited by other articles:


Home page
 CirculationHome page
S. Lavi, J. P. McConnell, C. S. Rihal, A. Prasad, V. Mathew, L. O. Lerman, and A. Lerman
Local Production of Lipoprotein-Associated Phospholipase A2 and Lysophosphatidylcholine in the Coronary Circulation: Association With Early Coronary Atherosclerosis and Endothelial Dysfunction in Humans
Circulation, May 29, 2007; 115(21): 2715 - 2721.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S.-i. Saitoh, C. Zhang, J. D. Tune, B. Potter, T. Kiyooka, P. A. Rogers, J. D. Knudson, G. M. Dick, A. Swafford, and W. M. Chilian
Hydrogen Peroxide: A Feed-Forward Dilator That Couples Myocardial Metabolism to Coronary Blood Flow
Arterioscler. Thromb. Vasc. Biol., December 1, 2006; 26(12): 2614 - 2621.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.