AJP - Heart AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol (August 26, 2005). doi:10.1152/ajpheart.00457.2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
290/1/H232    most recent
00457.2005v2
00457.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lindsey, M. L
Right arrow Articles by Spinale, F. G
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lindsey, M. L
Right arrow Articles by Spinale, F. G
Submitted on May 6, 2005
Accepted on August 19, 2005

Matrix Metalloproteinase-9 Gene Deletion Facilitates Angiogenesis Following Myocardial Infarction

Merry L Lindsey1*, G. Patricia Escobar1, Wawrzyniec L Dobrucki2, Danielle K Goshorn1, Shenikqua Bouges1, Joseph T Mingoia1, David M McClister, Jr.1, Haili Su2, Joseph Gannon3, Catherine MacGillivray3, Richard T Lee3, Albert J Sinusas2, and Francis G Spinale4

1 Cardiothoracic Surgery Research, Medical University of South Carolina, Charleston, SC, USA
2 Cardiovascular Medicine, Yale University School of Medicine, New Haven, CT, USA
3 Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA
4 Cardiothoracic Surgery Research, Medical University of South Carolina, Charleston, SC, USA; Ralph A. Johnson Veterans Administration Medical Center, Charleston, SC, USA

* To whom correspondence should be addressed. E-mail: lindseml{at}musc.edu.

Matrix metalloproteinases (MMPs) are postulated to be necessary for neovascularization during wound healing. MMP-9 deletion alters remodeling post-myocardial infarction (MI), but whether and to what degree MMP-9 affects neovascularization post-MI is unknown. Neovascularization was evaluated in wild-type (WT;n=63) and MMP-9-null mice (n=55) at 7-days post-MI. Despite similar infarct sizes, MMP-9 deletion improved left ventricular function as evaluated by hemodynamic analysis. Blood vessel quantity and quality was evaluated by three independent studies. First, vessel density was increased in the infarct of MMP-9-null mice compared with WT, as quantified by Griffonia (Bandeiraea) Simplicifolia Lectin-I (GSL-I) immunohistochemistry. Second, pre-existing vessels, stained in vivo with FITC-labeled GSL-I pre-MI, were present in the viable but not MI region. Third, a technetium-99m labeled peptide (NC100692), which selectively binds to activated {alpha}v{beta}3 integrin in angiogenic vessels, was injected into post-MI mice. Relative NC100692 activity in myocardial segments with diminished perfusion (0-40% non-ischemic) was higher in MMP-9-null than WT mice (383±162% vs. 250±118%, respectively;p=0.002). The unique finding of this study was that MMP-9 deletion stimulated, rather than impaired, neovascularization in remodeling myocardium. Thus, targeted strategies to inhibit MMP-9 early post-MI will likely not impair the angiogenic response.




This article has been cited by other articles:


Home page
 Circ. Res.Home page
P. Krishnamurthy, J. Rajasingh, E. Lambers, G. Qin, D. W. Losordo, and R. Kishore
IL-10 Inhibits Inflammation and Attenuates Left Ventricular Remodeling After Myocardial Infarction via Activation of STAT3 and Suppression of HuR
Circ. Res., January 30, 2009; 104(2): e9 - e18.
[Abstract] [Full Text] [PDF]

Home page
 JNMHome page
B. L. Franc, P. D. Acton, C. Mari, and B. H. Hasegawa
Small-Animal SPECT and SPECT/CT: Important Tools for Preclinical Investigation
J. Nucl. Med., October 1, 2008; 49(10): 1651 - 1663.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll Cardiol ImgHome page
A. J. Sinusas
Targeted imaging offers advantages over physiological imaging for evaluation of angiogenic therapy.
J. Am. Coll. Cardiol. Img., July 1, 2008; 1(4): 511 - 514.
[Full Text] [PDF]

Home page
 Physiol. Rev.Home page
F. G. Spinale
Myocardial Matrix Remodeling and the Matrix Metalloproteinases: Influence on Cardiac Form and Function
Physiol Rev, October 1, 2007; 87(4): 1285 - 1342.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. Cretu, J. M. Roth, M. Caunt, A. Akalu, D. Policarpio, S. Formenti, P. Gagne, L. Liebes, and P. C. Brooks
Disruption of Endothelial Cell Interactions with the Novel HU177 Cryptic Collagen Epitope Inhibits Angiogenesis
Clin. Cancer Res., May 15, 2007; 13(10): 3068 - 3078.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Goruppi, R. D. Patten, T. Force, and J. M. Kyriakis
Helix-Loop-Helix Protein p8, a Transcriptional Regulator Required for Cardiomyocyte Hypertrophy and Cardiac Fibroblast Matrix Metalloprotease Induction
Mol. Cell. Biol., February 1, 2007; 27(3): 993 - 1006.
[Abstract] [Full Text] [PDF]

Home page
 JNMHome page
T. Bach-Gansmo, R. Danielsson, A. Saracco, B. Wilczek, T. V. Bogsrud, A. Fangberget, A. Tangerud, and D. Tobin
Integrin Receptor Imaging of Breast Cancer: A Proof-of-Concept Study to Evaluate 99mTc-NC100692
J. Nucl. Med., September 1, 2006; 47(9): 1434 - 1439.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
P. Kanellakis, N. J. Slater, X.-J. Du, A. Bobik, and D. J. Curtis
Granulocyte colony-stimulating factor and stem cell factor improve endogenous repair after myocardial infarction
Cardiovasc Res, April 1, 2006; 70(1): 117 - 125.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.