AJP - Heart AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol (January 24, 2002). doi:10.1152/ajpheart.00471.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/3/H821    most recent
00471.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by BOUDINA, S.
Right arrow Articles by DOS SANTOS, P.
Right arrow Search for Related Content
PubMed
Right arrow Articles by BOUDINA, S.
Right arrow Articles by DOS SANTOS, P.

Articles in PresS, published online ahead of print January 24, 2002
Am J Physiol Heart Circ Physiol, 10.1152/ajpheart.00471.2001
Submitted on May 31, 2001
Accepted on September 19, 2001

ALTERATION OF MITOCHONDRIAL FUNCTION IN A MODELE OF CHRONIC ISCHEMIA IN VIVO IN RAT HEART

SIHEM BOUDINA1, MURIEL N LACLAU1, LILIANE TARIOSSE1, DANNIELE DARET1, GERARD GOUVERNEUR1, SIMONE BONORON-ADELE1, VALDUR A SAKS2, and PIERRE DOS SANTOS1*

1 Atherosclerosis, INSERM U441, Pessac, France, France
2 FourrierLaboratory of Bioenergetics, Universite Joseph, Grenoble, France, France; Laboratory of Bioenergetics, National Institute of Chemical and Biological Physics, Tallinn, Estonia, Estonia

The aim of this study is to investigate mitochondrial alterations in an animal model of chronic myocardial ischemia in rats obtained by surgical constriction of left coronary artery. Resting coronary blood flow was measured using the fluorescent microsphere technique. Contractile function, defined by rate pressure product, and myocardial oxygen consumption were measured in a Langendorff preparation. The mitochondrial function was evaluated on permeabilized skinned fibers. Three weeks after surgery, ischemic hearts showed a significant decrease in coronary blood flow compared to sham. Hemodynamic measurements showed a significant systolic and diastolic dysfunction. Alterations in mitochondrial function in ischemic hearts were mainly characterized by a significant decrease in Vmax and apparent K1/2 for ADP, loss of the stimulatory effect of creatine and a stimulatory effect of exogenous cytochrome c. These functional alterations were supported by structural alterations characterized by mitochondrial clustering and swelling associated with membrane rupture. We conclude that the alterations in systolic function after chronic ischemia are supported by severe modifications of mitochondrial structure and function.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Birkedal, H. A. Shiels, and M. Vendelin
Three-dimensional mitochondrial arrangement in ventricular myocytes: from chaos to order
Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1148 - C1158.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
X. Fang, W. Tang, S. Sun, L. Huang, Y.-T. Chang, Z. Huang, and M. H. Weil
Cardiopulmonary resuscitation in a rat model of chronic myocardial ischemia
J Appl Physiol, October 1, 2006; 101(4): 1091 - 1096.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Vendelin, N. Beraud, K. Guerrero, T. Andrienko, A. V. Kuznetsov, J. Olivares, L. Kay, and V. A. Saks
Mitochondrial regular arrangement in muscle cells: a "crystal-like" pattern
Am J Physiol Cell Physiol, March 1, 2005; 288(3): C757 - C767.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.