AJP - Heart Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol (January 13, 2006). doi:10.1152/ajpheart.00486.2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
290/6/H2320    most recent
00486.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gambillara, V.
Right arrow Articles by Silacci, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gambillara, V.
Right arrow Articles by Silacci, P.
Submitted on May 11, 2005
Accepted on December 26, 2005

Plaque-prone hemodynamic impairs endothelial function in pig carotid arteries

Veronica Gambillara1*, Celine Chambaz1, Sylvain Roy1, Nikos Stergiopulos1, and Paolo Silacci1

1 Swiss Federal Institute of Technology, Laboratory of Hemodynamics and Cardiovascular Technology, Lausanne, Vaud, Switzerland

* To whom correspondence should be addressed. E-mail: veronica.gambillara{at}epfl.ch.

Hemodynamic forces play an active role in vascular pathologies, particularly in relation to the localization of atherosclerotic lesions. It has been established that low shear stress combined with cyclic reversal of flow direction (oscillatory shear stress) affects the endothelial cells and may lead to an initiation of plaque development. The aim of the study was to analyze the effect of hemodynamic conditions in arterial segments perfused in vitro, in the absence of other stimuli. Left common porcine carotid segments were mounted into an ex-vivo arterial support system and perfused 3 days under unidirectional high and low shear stress (6± 3 and 0.3± 0.1 dynes/cm2) or oscillatory shear stress (0.3± 3 dynes/cm2). Bradykinin (BK)-induced vasorelaxation was drastically decreased in arteries exposed to oscillatory shear stress as compared to unidirectional shear stress. Impaired nitric oxide (NO)-mediated vasodilation was correlated to changes in both eNOS gene expression and eNOS activation in response to BK. This study determined the flow-mediated effects on native tissue cells perfused with physiologically relevant flows and supports the hypothesis that oscillatory shear stress is a determinant factor in early stages of atherosclerosis. Indeed, oscillatory shear stress induces an endothelial dysfunction, whereas unidirectional shear stress preserves the function of endothelial cells. Endothelial dysfunction is directly mediated by a down-regulation of eNOS gene expression and activation; consequently, a decrease of NO production and/or bioavailability occurs.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. R. Lawrence and K. J. Gooch
Transmural pressure and axial loading interactively regulate arterial remodeling ex vivo
Am J Physiol Heart Circ Physiol, July 1, 2009; 297(1): H475 - H484.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J.-L. Balligand, O. Feron, and C. Dessy
eNOS Activation by Physical Forces: From Short-Term Regulation of Contraction to Chronic Remodeling of Cardiovascular Tissues
Physiol Rev, April 1, 2009; 89(2): 481 - 534.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Ai, M. Rouhanizadeh, J. C. Wu, W. Takabe, H. Yu, M. Alavi, R. Li, Y. Chu, J. Miller, D. D. Heistad, et al.
Shear stress influences spatial variations in vascular Mn-SOD expression: implication for LDL nitration
Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1576 - C1585.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. E. Pyke, V. Poitras, and M. E. Tschakovsky
Brachial artery flow-mediated dilation during handgrip exercise: evidence for endothelial transduction of the mean shear stimulus
Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2669 - H2679.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. C. Newcomer, C. L. Sauder, N. T. Kuipers, M. H. Laughlin, and C. A. Ray
Effects of posture on shear rates in human brachial and superficial femoral arteries
Am J Physiol Heart Circ Physiol, April 1, 2008; 294(4): H1833 - H1839.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. H. Laughlin, S. C. Newcomer, and S. B. Bender
Importance of hemodynamic forces as signals for exercise-induced changes in endothelial cell phenotype
J Appl Physiol, March 1, 2008; 104(3): 588 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
D. Won, S.-N. Zhu, M. Chen, A.-M. Teichert, J. E. Fish, C. C. Matouk, M. Bonert, M. Ojha, P. A. Marsden, and M. I. Cybulsky
Relative Reduction of Endothelial Nitric-Oxide Synthase Expression and Transcription in Atherosclerosis-Prone Regions of the Mouse Aorta and in an in Vitro Model of Disturbed Flow
Am. J. Pathol., November 1, 2007; 171(5): 1691 - 1704.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
Y. S. Chatzizisis, A. U. Coskun, M. Jonas, E. R. Edelman, C. L. Feldman, and P. H. Stone
Role of Endothelial Shear Stress in the Natural History of Coronary Atherosclerosis and Vascular Remodeling: Molecular, Cellular, and Vascular Behavior
J. Am. Coll. Cardiol., June 26, 2007; 49(25): 2379 - 2393.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.