| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Biochemistry, University of Iowa, Iowa City, IA, USA
2 Internal Medicine, University of Iowa, Iowa City, IA, USA
3 Entomology, University of California, Davis, CA, USA
4 Biochemistry, University of Iowa, Iowa City, IA, USA; Internal Medicine, University of Iowa, Iowa City, IA, USA
* To whom correspondence should be addressed. E-mail: xiang-fang{at}uiowa.edu.
We investigated the effects of soluble epoxide hydrolase (sEH) inhibition on epoxyeicosatrienoic acid (EET) metabolism in intact human blood vessels, including saphenous vein (HSV), coronary artery (HCA), and aorta (HA). When HSV segments were perfused with 2 µmol/L [3H]14,15-EET for 4 h, > 60% of radioactivity in the perfusion medium was converted to 14,15-dihydroxyeicosatrienoic acid (DHET). Similar results were obtained with endothelium-denuded vessels. 14,15-DHET was released from both the luminal and adventitial surfaces of the HSV. When HSV were incubated with [3H]14,15-EET under static (no flow) conditions, formation of 14,15-DHET was detected within 15 min and was inhibited by the selective sEH inhibitors N,N'-dicyclohexyl urea and N-cyclohexyl-N'-dodecanoic acid urea (CUDA). Similarly, CUDA inhibited the conversion of [3H]11,12-EET to 11,12-DHET by HSV. sEH inhibition enhanced the uptake of [3H]14,15-EET and facilitated the formation of 10,11-epoxy-16:2, a 
-oxidation product. HCA and HA converted [3H]14,15-EET to DHET, and this also was inhibited by CUDA. These findings in intact human blood vessels indicate that conversion to DHET is the predominant pathway for 11,12- and 14,15-EET metabolism and that sEH inhibition can modulate EET metabolism in the vascular tissue.
This article has been cited by other articles:
|
|
 |
|
  K. R. Chaudhary, S. N. Batchu, D. Das, M. R. Suresh, J. R. Falck, J. P. Graves, D. C. Zeldin, and J. M. Seubert Role of B-type natriuretic peptide in epoxyeicosatrienoic acid-mediated improved post-ischaemic recovery of heart contractile function Cardiovasc Res, July 15, 2009; 83(2): 362 - 370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Motoki, M. J. Merkel, W. H. Packwood, Z. Cao, L. Liu, J. Iliff, N. J. Alkayed, and D. M. Van Winkle Soluble epoxide hydrolase inhibition and gene deletion are protective against myocardial ischemia-reperfusion injury in vivo Am J Physiol Heart Circ Physiol, November 1, 2008; 295(5): H2128 - H2134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Corenblum, V. E. Wise, K. Georgi, B. D. Hammock, P. A. Doris, and M. Fornage Altered Soluble Epoxide Hydrolase Gene Expression and Function and Vascular Disease Risk in the Stroke-Prone Spontaneously Hypertensive Rat Hypertension, February 1, 2008; 51(2): 567 - 573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. T. Larsen, D. D. Gutterman, A. Sato, K. Toyama, W. B. Campbell, D. C. Zeldin, V. L. Manthati, J. R. Falck, and H. Miura Hydrogen Peroxide Inhibits Cytochrome P450 Epoxygenases: Interaction Between Two Endothelium-Derived Hyperpolarizing Factors Circ. Res., January 4, 2008; 102(1): 59 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Spector and A. W. Norris Action of epoxyeicosatrienoic acids on cellular function Am J Physiol Cell Physiol, March 1, 2007; 292(3): C996 - C1012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Fang, F. M. Faraci, T. L. Kaduce, S. Harmon, M. L. Modrick, S. Hu, S. A. Moore, J. R. Falck, N. L. Weintraub, and A. A. Spector 20-Hydroxyeicosatetraenoic acid is a potent dilator of mouse basilar artery: role of cyclooxygenase Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2301 - H2307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Seubert, C. J. Sinal, J. Graves, L. M. DeGraff, J. A. Bradbury, C. R. Lee, K. Goralski, M. A. Carey, A. Luria, J. W. Newman, et al. Role of Soluble Epoxide Hydrolase in Postischemic Recovery of Heart Contractile Function Circ. Res., August 18, 2006; 99(4): 442 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Lee, K. E. North, M. S. Bray, M. Fornage, J. M. Seubert, J. W. Newman, B. D. Hammock, D. J. Couper, G. Heiss, and D. C. Zeldin Genetic variation in soluble epoxide hydrolase (EPHX2) and risk of coronary heart disease: The Atherosclerosis Risk in Communities (ARIC) study Hum. Mol. Genet., May 15, 2006; 15(10): 1640 - 1649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. T. Larsen, H. Miura, O. A. Hatoum, W. B. Campbell, B. D. Hammock, D. C. Zeldin, J. R. Falck, and D. D. Gutterman Epoxyeicosatrienoic and dihydroxyeicosatrienoic acids dilate human coronary arterioles via BKCa channels: implications for soluble epoxide hydrolase inhibition Am J Physiol Heart Circ Physiol, February 1, 2006; 290(2): H491 - H499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Fang, S. Hu, B. Xu, G. D. Snyder, S. Harmon, J. Yao, Y. Liu, B. Sangras, J. R. Falck, N. L. Weintraub, et al. 14,15-Dihydroxyeicosatrienoic acid activates peroxisome proliferator-activated receptor-{alpha} Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H55 - H63. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Vriens, G. Owsianik, B. Fisslthaler, M. Suzuki, A. Janssens, T. Voets, C. Morisseau, B.D. Hammock, I. Fleming, R. Busse, et al. Modulation of the Ca2 Permeable Cation Channel TRPV4 by Cytochrome P450 Epoxygenases in Vascular Endothelium Circ. Res., October 28, 2005; 97(9): 908 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Jung, R. P. Brandes, I.-H. Kim, F. Schweda, R. Schmidt, B. D. Hammock, R. Busse, and I. Fleming Soluble Epoxide Hydrolase Is a Main Effector of Angiotensin II-Induced Hypertension Hypertension, April 1, 2005; 45(4): 759 - 765. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
