AJP - Heart AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol (August 21, 2003). doi:10.1152/ajpheart.00547.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/6/H2688    most recent
00547.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhong, Y.
Right arrow Articles by Matlib, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhong, Y.
Right arrow Articles by Matlib, M. A.
Submitted on June 12, 2003
Accepted on July 23, 2003

Gender Differences in Myosin Heavy Chain-{beta} and Phosphorylated Phospholamban in Diabetic Rat Hearts

Yan Zhong1, Peter J. Reiser2, and Mohammed A. Matlib1*

1 Department of Pharmacology and Cell Biophysics, University of Cincinnati, Cincinnati, OH, USA
2 Department of Oral Biology, Ohio State University, Columbus, OH, USA

* To whom correspondence should be addressed. E-mail: matlibma{at}email.uc.edu.

The objective of the study was to determine whether a gender difference exists either in myosin heavy chain (MHC) isoform or in sarcoplasmic reticulum (SR) protein levels in diabetic rat hearts. As is the case of normal rodent hearts, all four chambers of the control rat hearts had almost 100% MHC-{alpha}. MHC-{beta} expression in ventricles of male 6-week diabetic rats was significantly greater (78 ± 7 %) compared to that of female 6-week diabetic rats (50 ± 5 %). The cardiac SR Ca2+-ATPase (SERCA2a) protein level was decreased and the phospholamban (PLB) protein level was increased in the left ventricle of diabetic rats, but there was no difference between male and female diabetic rats. The phosphorylated PLB level was decreased more in male diabetic rats than in female diabetic rats. Insulin treatment completely normalized blood glucose level and cardiac SERCA2a and PLB protein levels, and the decrease in MHC-{beta} level in both male and female diabetic rats. Insulin treatment completely normalized serum insulin and almost completely normalized phosphorylation of PLB at serine-16 in male diabetic rats. Insulin treatment completely normalized serum insulin level in male diabetic rats, but only partially normalized in female diabetic rats. Although insulin treatment almost completely normalized phosphorylation of PLB at threonine-17 in female diabetic rats, the increase was significantly more than insulin-treated male diabetic rats. It is concluded that higher levels of MHC-b and dephosphorylated PLB may contribute to more contractile dysfunction in the male diabetic rat hearts than female diabetic rat hearts and that phosphorylation of PLB at threonine-17 more responsive to insulin treatment in female diabetic rat hearts.




This article has been cited by other articles:


Home page
 Cardiovasc ResHome page
M. Periasamy, P. Bhupathy, and G. J. Babu
Regulation of sarcoplasmic reticulum Ca2+ ATPase pump expression and its relevance to cardiac muscle physiology and pathology
Cardiovasc Res, January 15, 2008; 77(2): 265 - 273.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
N. Yaras, E. Tuncay, N. Purali, B. Sahinoglu, G. Vassort, and B. Turan
Sex-related effects on diabetes-induced alterations in calcium release in the rat heart
Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3584 - H3592.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. F. Ceylan-Isik, K. H. LaCour, and J. Ren
Sex difference in cardiomyocyte function in normal and metallothionein transgenic mice: the effect of diabetes mellitus
J Appl Physiol, May 1, 2006; 100(5): 1638 - 1646.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.