| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
in chronically hypoxic rat myocardium: involvement in cardioprotection
1 Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
2 Centre for Experimental Cardiovascular Research, Prague, Czech Republic
* To whom correspondence should be addressed. E-mail: kolar{at}biomed.cas.cz.
We examined the role of protein kinase C (PKC) in the cardioprotective mechanism induced by long-term adaptation to chronic intermittent hypoxia. Adult male Wistar rats were exposed to hypobaric hypoxia of 7000 m for 8 h/day, 5 days/week; the total number of exposures was 24-32. A control group was kept under normoxic conditions. The Western blot analysis of PKC isoforms 
and 
was performed in the cytosol and three particulate fractions of left ventricular myocardium. Infarct size was determined in open-chest animals subjected to 20-min coronary artery occlusion and 3-h reperfusion. PKC inhibitors chelerythrine (1 mg/kg or 5
mg/kg) or rottlerin (selective for PKC isoform; 0.3 mg/kg) were administered intravenously as a
single bolus 15 min before ischemia. Chronic hypoxia had no effect on the expression and distribution of PKC. The relative amount of PKC increased in the cytosol and nuclearcytoskeletal,
mitochondrial and microsomal fractions of chronically hypoxic myocardium by 100%, 212%, 237%, and 146%, respectively, compared to corresponding normoxic values.
Chronic hypoxia decreased the size of myocardial infarction (normalized to the area at risk) by
about one-third on the average (P<0.05). Both doses of chelerythrine tended to reduce infarction
in controls and only its high dose completely abolished the improvement of ischemic tolerance in
hypoxic hearts (P<0.05). Rottlerin attenuated the infarct size-limiting effect of chronic hypoxia
(P<0.05) and it had no effect in controls. These results suggest that chronic intermittent hypoxiainduced
cardioprotection in rats is partially mediated by PKC; the contribution of other isoforms remains to be determined.
This article has been cited by other articles:
|
|
 |
|
  E. Gurel, K. M. Smeele, O. Eerbeek, A. Koeman, C. Demirci, M. W. Hollmann, and C. J. Zuurbier Ischemic preconditioning affects hexokinase activity and HKII in different subcellular compartments throughout cardiac ischemia-reperfusion J Appl Physiol, June 1, 2009; 106(6): 1909 - 1916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. House, S. J. Melhorn, G. Newman, T. Doetschman, and J. E. J. Schultz The protein kinase C pathway mediates cardioprotection induced by cardiac-specific overexpression of fibroblast growth factor-2 Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H354 - H365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hlavackova, J. Neckar, J. Jezkova, P. Balkova, B. Stankova, O. Novakova, F. Kolar, and F. Novak Dietary Polyunsaturated Fatty Acids Alter Myocardial Protein Kinase C Expression and Affect Cardioprotection Induced by Chronic Hypoxia Experimental Biology and Medicine, June 1, 2007; 232(6): 823 - 832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. Yeung, G. M. Kravtsov, K. M. Ng, T. M. Wong, and M. L. Fung Chronic intermittent hypoxia alters Ca2+ handling in rat cardiomyocytes by augmented Na+/Ca2+ exchange and ryanodine receptor activities in ischemia-reperfusion Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2046 - C2056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Kolar, J. Jezkova, P. Balkova, J. Breh, J. Neckar, F. Novak, O. Novakova, H. Tomasova, M. Srbova, B. Ost'adal, et al. Role of oxidative stress in PKC-{delta} upregulation and cardioprotection induced by chronic intermittent hypoxia Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H224 - H230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Watanabe, H. Yaoita, K. Ogawa, M. Oikawa, K. Maehara, and Y. Maruyama Attenuated cardioprotection by ischemic preconditioning in coronary stenosed heart and its restoration by carvedilol Cardiovasc Res, August 1, 2006; 71(3): 537 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chen, X.-Y. Lu, J. Li, J.-D. Fu, Z.-N. Zhou, and H.-T. Yang Intermittent hypoxia protects cardiomyocytes against ischemia-reperfusion injury-induced alterations in Ca2+ homeostasis and contraction via the sarcoplasmic reticulum and Na+/Ca2+ exchange mechanisms Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1221 - C1229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. E. Breitwieser GIRK channels: hierarchy of control. Focus on "PKC-{delta} sensitizes Kir3.1/3.2 channels to changes in membrane phospholipid levels after M3 receptor activation in HEK-293 cells" Am J Physiol Cell Physiol, September 1, 2005; 289(3): C509 - C511. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
