| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Bionengineering, University of California, San Diego, La Jolla, CA, USA
2 Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA
* To whom correspondence should be addressed. E-mail: pjohnson{at}bioeng.ucsd.edu.
In previous studies we have shown that intravenous infusion of Dextran 500 in the rat causes blunting of the velocity profile of red blood cells in venules at low shear rates. To determine whether this blunting is associated with the formation of red blood cell aggregates, we measured the length and width of particles in the venular flow stream at systemic hematocrits up to 20% using a high speed video camera and a new image analysis technique. Data were obtained at various shear rates under normal (non-aggregating) conditions as well as after infusion of Dextran 500. Under normal conditions, particle length (parallel to the vessel axis) was 6.5 ± 2.7 µm and width (perpendicular to the axis) was 6.1 ± 1.7 µµm in agreement published dimensions of individual red blood cells for this species. After Dextran 500 infusion, particle length and width increased significantly to 8.7 ± 5.1 µm and 10.4 ± 4.4 µm, respectively. Particle dimensions were greater in the central region of the flow stream for both normal and Dextran-treated blood and increased at low flow rates with Dextran-treated blood. This study provides direct confirmation of aggregate formation at low shear in venules with high molecular weight Dextran as well as an estimate of aggregate size and range.
This article has been cited by other articles:
|
|
 |
|
  D. K. Kaul, A. Koshkaryev, G. Artmann, G. Barshtein, and S. Yedgar Additive effect of red blood cell rigidity and adherence to endothelial cells in inducing vascular resistance Am J Physiol Heart Circ Physiol, October 1, 2008; 295(4): H1788 - H1793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kim, J. Zhen, A. S. Popel, M. Intaglietta, and P. C. Johnson Contributions of collision rate and collision efficiency to erythrocyte aggregation in postcapillary venules at low flow rates Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1947 - H1954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kim, A. S. Popel, M. Intaglietta, and P. C. Johnson Effect of erythrocyte aggregation at normal human levels on functional capillary density in rat spinotrapezius muscle Am J Physiol Heart Circ Physiol, March 1, 2006; 290(3): H941 - H947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kim, A. S. Popel, M. Intaglietta, and P. C. Johnson Aggregate formation of erythrocytes in postcapillary venules Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H584 - H590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Yalcin, M. Uyuklu, J. K. Armstrong, H. J. Meiselman, and O. K. Baskurt Graded alterations of RBC aggregation influence in vivo blood flow resistance Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2644 - H2650. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
