|
|
||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* To whom correspondence should be addressed. E-mail: mike_wolin{at}nymc.edu.
Since systems controlled by basal NAD(P)H oxidase activity appear to contribute to differences in responses of endothelium-removed bovine coronary (BCA) and pulmonary (BPA) arteries to hypoxia, we characterized the Nox-oxidases activities present in these vascular segments and how cytosolic NAD(P)H redox systems could be controlling oxidase activity. BPA generated approximately 60-80% more lucigenin (5 µM) chemiluminescence detectable superoxide than BCA. Apocynin (10 µM), a NAD(P)H oxidase inhibitor, and 6-aminonicotinamide (1 mM), a pentose phosphate inhibitor (PPP), both attenuated (approximately by 50-70 %) superoxide detected in BPA and BCA. There was no significant difference in the expression of Nox-2 or Nox-4 mRNA or protein detected by western blot analysis. NADPH and NADH increased superoxide in homogenates and isolated microsomal membrane fractions in a manner consistent with BPA and BCA having similar levels of oxidase activity. BPA had 4.2-fold higher levels of NADPH than BCA. The activity and protein levels of glucose-6-phosphate dehydrogenase (G6PD), the rate limiting PPP enzyme generating cytosolic NADPH, were 1.5-fold higher in BPA than BCA. Thus, BPA differ from BCA in that they have higher levels of G6PD activity, NADPH, and superoxide. Since both arteries have similar levels of Nox expression and activity, elevated levels of cytosolic NADPH may contribute to increased superoxide in BPA.
This article has been cited by other articles:
![]() |
Q. Gao, X. Zhao, M. Ahmad, and M. S. Wolin Mitochondrial-derived hydrogen peroxide inhibits relaxation of bovine coronary arterial smooth muscle to hypoxia through stimulation of ERK MAP kinase Am J Physiol Heart Circ Physiol, December 1, 2009; 297(6): H2262 - H2269. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Ahmad, X. Zhao, M. R. Kelly, S. Kandhi, O. Perez, N. G. Abraham, and M. S. Wolin Heme oxygenase-1 induction modulates hypoxic pulmonary vasoconstriction through upregulation of ecSOD Am J Physiol Heart Circ Physiol, October 1, 2009; 297(4): H1453 - H1461. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. J. McCrann, A. Eliades, M. Makitalo, K. Matsuno, and K. Ravid Differential expression of NADPH oxidases in megakaryocytes and their role in polyploidy Blood, August 6, 2009; 114(6): 1243 - 1249. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Serpillon, B. C. Floyd, R. S. Gupte, S. George, M. Kozicky, V. Neito, F. Recchia, W. Stanley, M. S. Wolin, and S. A. Gupte Superoxide production by NAD(P)H oxidase and mitochondria is increased in genetically obese and hyperglycemic rat heart and aorta before the development of cardiac dysfunction. The role of glucose-6-phosphate dehydrogenase-derived NADPH Am J Physiol Heart Circ Physiol, July 1, 2009; 297(1): H153 - H162. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. K Schwartz, J. C Lieske, L. W. Hunter, and V. M Miller Systemic injection of planktonic forms of mammalian-derived nanoparticles alters arterial response to injury in rabbits Am J Physiol Heart Circ Physiol, May 1, 2009; 296(5): H1434 - H1441. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. A. Gupte, P. M. Kaminski, S. George, L. Kouznestova, S. C. Olson, R. Mathew, T. H. Hintze, and M. S. Wolin Peroxide generation by p47phox-Src activation of Nox2 has a key role in protein kinase C-induced arterial smooth muscle contraction Am J Physiol Heart Circ Physiol, April 1, 2009; 296(4): H1048 - H1057. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. S. Wolin Reactive oxygen species and the control of vascular function Am J Physiol Heart Circ Physiol, March 1, 2009; 296(3): H539 - H549. [Abstract] [Full Text] [PDF] |
||||
![]() |
Q. Gao and M. S. Wolin Effects of hypoxia on relationships between cytosolic and mitochondrial NAD(P)H redox and superoxide generation in coronary arterial smooth muscle Am J Physiol Heart Circ Physiol, September 1, 2008; 295(3): H978 - H989. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Csiszar, N. Labinskyy, H. Jo, P. Ballabh, and Z. Ungvari Differential proinflammatory and prooxidant effects of bone morphogenetic protein-4 in coronary and pulmonary arterial endothelial cells Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H569 - H577. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Li, D. H. Kim, P. L. Tsenovoy, S. J. Peterson, R. Rezzani, L. F. Rodella, W. S. Aronow, S. Ikehara, and N. G. Abraham Treatment of Obese Diabetic Mice With a Heme Oxygenase Inducer Reduces Visceral and Subcutaneous Adiposity, Increases Adiponectin Levels, and Improves Insulin Sensitivity and Glucose Tolerance Diabetes, June 1, 2008; 57(6): 1526 - 1535. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. Huang, P. M. Kaminski, J. G. Edwards, A. Yeh, M. S. Wolin, W. H. Frishman, M. H. Gewitz, and R. Mathew Pyrrolidine dithiocarbamate restores endothelial cell membrane integrity and attenuates monocrotaline-induced pulmonary artery hypertension Am J Physiol Lung Cell Mol Physiol, June 1, 2008; 294(6): L1250 - L1259. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Medhora, Y. Chen, S. Gruenloh, D. Harland, S. Bodiga, J. Zielonka, D. Gebremedhin, Y. Gao, J. R. Falck, S. Anjaiah, et al. 20-HETE increases superoxide production and activates NAPDH oxidase in pulmonary artery endothelial cells Am J Physiol Lung Cell Mol Physiol, May 1, 2008; 294(5): L902 - L911. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. L. Moens, H. C. Champion, M. J. Claeys, B. Tavazzi, P. M. Kaminski, M. S. Wolin, D. J. Borgonjon, L. Van Nassauw, A. Haile, M. Zviman, et al. High-Dose Folic Acid Pretreatment Blunts Cardiac Dysfunction During Ischemia Coupled to Maintenance of High-Energy Phosphates and Reduces Postreperfusion Injury Circulation, April 8, 2008; 117(14): 1810 - 1819. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. J. Mingone, M. Ahmad, S. A. Gupte, J. L. Chow, and M. S. Wolin Heme oxygenase-1 induction depletes heme and attenuates pulmonary artery relaxation and guanylate cyclase activation by nitric oxide Am J Physiol Heart Circ Physiol, March 1, 2008; 294(3): H1244 - H1250. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. L. Archer, M. Gomberg-Maitland, M. L. Maitland, S. Rich, J. G. N. Garcia, and E. K. Weir Mitochondrial metabolism, redox signaling, and fusion: a mitochondria-ROS-HIF-1{alpha}-Kv1.5 O2-sensing pathway at the intersection of pulmonary hypertension and cancer Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H570 - H578. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. Hodyc, M. Snorek, T. Brtnicky, and J. Herget Respiratory: Superoxide dismutase mimetic tempol inhibits hypoxic pulmonary vasoconstriction in rats independently of nitric oxide production Exp Physiol, September 1, 2007; 92(5): 945 - 951. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Zhang, F. Zhang, R. Muh, F. Yi, K. Chalupsky, H. Cai, and P.-L. Li Autocrine/paracrine pattern of superoxide production through NAD(P)H oxidase in coronary arterial myocytes Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H483 - H495. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. T. Larsen and D. D. Gutterman Hypoxia, coronary dilation, and the pentose phosphate pathway Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2169 - H2171. [Full Text] [PDF] |
||||
![]() |
S. A. Gupte and M. S. Wolin Hypoxia promotes relaxation of bovine coronary arteries through lowering cytosolic NADPH Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2228 - H2238. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. S. Wolin, M. Ahmad, and S. A. Gupte Oxidant and redox signaling in vascular oxygen sensing mechanisms: basic concepts, current controversies, and potential importance of cytosolic NADPH Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L159 - L173. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Turkseven, A. Kruger, C. J. Mingone, P. Kaminski, M. Inaba, L. F. Rodella, S. Ikehara, M. S. Wolin, and N. G. Abraham Antioxidant mechanism of heme oxygenase-1 involves an increase in superoxide dismutase and catalase in experimental diabetes Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H701 - H707. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Kinugawa, J. Zhang, E. Messina, E. Walsh, H. Huang, P. M. Kaminski, M. S. Wolin, and T. H. Hintze gp91phox-containing NAD(P)H oxidase mediates attenuation of nitric oxide-dependent control of myocardial oxygen consumption by ANG II Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H862 - H867. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. S. Wilcox and D. Gutterman Focus on oxidative stress in the cardiovascular and renal systems Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H3 - H6. [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |