Understanding diastolic function mandates feasible and accurate methods to construct and compare the diastolic pressure (P)-volume (V) relationship (PVR). This study compared the relaxation-corrected single beat (RC-SB) to the multiple-beat (MB) (vena cava occlusion) method for constructing the diastolic PVR in 26 young normal (YN) or old hypertensive (OH) dogs before and after increases in afterload (phenylephrine) or acute volume expansion in the presence (n=14) or absence (n=12) of the pericardium. The PVR data were fit to P = e^()(V). Derived stiffness indices compared included , , and the end-diastolic volume (EDV) at P=10, 20, or 30 mmHg (EDV_x=Ln(P_x/)/) to account for covariance in and . In pericardium intact YN and OH dogs studied over varying afterloads, the MB and RC-SB PVR appeared identical. The (r=0.62) and (r=0.69) derived from the RC-SB vs MB PVR showed moderate correlation but poor agreement. In contrast, the EDV_10-30 derived from RC-SB vs MB PVR showed excellent correlation (r=0.97) and agreement. The uncorrected SB method underestimated stiffness. As expected, after acute volume expansion, the RC-SB PVR was shifted upward from the MB PVR (decreased EDV_10-30, p<0.05) in the pericardium intact but not pericardium absent dogs. The RC-SB method can substitute for MB technique in construction of PVR in absence of acute volume expansion. The concordance between these two methods was poorly reflected by comparing the derived &#945; and but apparent when using EDV_10-30 which provides information regarding the position of the PVR in a single number.