The biomechanical environment to which cells are exposed is important to their normal growth, development, interaction and function. Accordingly there has been much interest in studying the role of biomechanical forces in cell biology and pathophysiology. This has led to the introduction and even commercialization of many experimental devices. Many of the early devices were limited by the heterogeneity of deformation of cells cultivated in different locations of the culture plate membranes and were also attached with complicated technical/electronical efforts resulting in a restriction of the reproducibility of these devices. The objective of this study was to design and build a simple device to allow the application of dose dependent homogeneous equibiaxial static stretch to cells cultured on flexible silicone membranes to investigate biological and biomedical questions. In addition cultured neonatal rat atrial cardiomyocytes were stretched with the proposed device with different strain gradients. For the first time with this study we could demonstrate that stretch up to 21% caused dose dependent changes in biological markers such as the calcineurin activity, MCIP1, Kv4.2 and KChIP2 gene expression and I_to current densities but not the protein/DNA ratio and ANP mRNA. With both markers mentioned last dose dependent stretch alterations could only be achieved with stretch up to 13%. The here presented simple and low-cost device might be applied to a wide range of experimental settings in different fields of research.