AJP - Heart Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 QUICK SEARCH:   [advanced]


     


Am J Physiol Heart Circ Physiol (December 15, 2006). doi:10.1152/ajpheart.00708.2006
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Videos
Right arrow All Versions of this Article:
292/4/H1876    most recent
00708.2006v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Osaka, M.
Right arrow Articles by Yoshida, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Osaka, M.
Right arrow Articles by Yoshida, M.
Submitted on July 4, 2006
Accepted on December 7, 2006

Real time imaging of mechanically injured-femoral artery in mouse revealed a biphasic pattern of leukocyte accumulation

Mizuko Osaka1, Sumihiko Hagita1, Mihiko Haraguchi2, Mayumi Kajimura3, Makoto Suematsu4, and Masayuki Yoshida1*

1 Life Science and Bioethics Research Center, Tokyo Med Dent Univ, Tokyo, NA, Japan
2 Tokyo, NA, Japan; Life Science and Bioethics Research Center, Tokyo Med Dent Univ, Tokyo, NA, Japan
3 Biochemistry, Keio University, Tokyo, Japan
4 Biochemistry, Keio University, United States

* To whom correspondence should be addressed. E-mail: masavasc{at}tmd.ac.jp.

Wire injury of an artery has been recognized as a standard model of vascular inflammation and atherosclerosis; however, the mechanism of leukocyte recruitment has not been studied in this model. In this study, we documented the recruitment of leukocytes to the murine femoral artery after a wire injury. A transluminal mechanical injury was generated by inserting a wire to the femoral artery of male C57BL/6J mice. Mice, anesthetized and ventilated after tracheotomy, were protected from hypothermia by a warming lamp. Body temperatures and blood pH did not significantly change during the experiment. The interaction between Rhodamine-6G labeled leukocytes and the injured femoral artery was monitored using an epifluorescent microscope. The captured images were then evaluated using a computer-assisted image analysis program. In the absence of injury, virtually no leukocyte adhesion was observed. In contrast, the number of adherent leukocytes increased at 4 hours and at 24 hours after injury. The number of leukocyte adhesion was reduced when we examined those 72 hours after injury. The rolling flux of leukocytes increased at 4 hours and remained high up to 7 days, whereas its velocity was faster at 72 hours. Further, we identified another peak of leukocyte adhesion at 7 days after injury. Injection of anti-P-selectin antibody significantly reduced leukocyte adhesion at the early and the later phase. In conclusion, we have established a novel experimental system to directly observe leukocyte recruitment to the injured femoral artery. Our system revealed a previously undetected, unique profile of leukocyte recruitment during vascular injury.




This article has been cited by other articles:


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
H. Wang, W. Zhang, R. Tang, R. P. Hebbel, M. A. Kowalska, C. Zhang, J. D. Marth, M. Fukuda, C. Zhu, and Y. Huo
Core2 1-6-N-Glucosaminyltransferase-I Deficiency Protects Injured Arteries From Neointima Formation in ApoE-Deficient Mice
Arterioscler Thromb Vasc Biol, July 1, 2009; 29(7): 1053 - 1059.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Pathol.Home page
G. Li, J. M. Sanders, M. H. Bevard, Z. Sun, J. W. Chumley, E. V. Galkina, K. Ley, and I. J. Sarembock
CD40 Ligand Promotes Mac-1 Expression, Leukocyte Recruitment, and Neointima Formation after Vascular Injury
Am. J. Pathol., April 1, 2008; 172(4): 1141 - 1152.
[Abstract] [Full Text] [PDF]


Home page
HypertensionHome page
S. Hagita, M. Osaka, K. Shimokado, and M. Yoshida
Oxidative Stress in Mononuclear Cells Plays a Dominant Role in Their Adhesion to Mouse Femoral Artery After Injury
Hypertension, March 1, 2008; 51(3): 797 - 802.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.